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ChIP experiments were carried out according to standard protocols (Upstate Biotechnology) with a crosslinking step (1% formaldehyde at 37 °C), followed by a quenching step with 125 mm glycine. Chromatin was sheared using the Bioruptor sonicator (Diagenode). Sonicated chromatin (350 μg) was immunoprecipitated with antibodies against anti-C/EBPα antibody (Santa Cruz, sc-61 or Abcam, ab40764) or a nonspecific polyclonal antibody. Immune complexes were captured with protein A-agarose beads, and eluted in SDS buffer. Formaldehyde crosslinking was reversed, followed by DNA purification using phenol/chloroform/isoamyl alcohol (Sigma-Aldrich) and quantified by qRT-PCR using the following primers: distal Stamp2 promoter forward 5’-GTT CTT TTC TGG CCT ACA GAT AGT-3’, reverse 5’- GGC ATC TCA CTC CTT AA GAG ACT-3’; Proximal Stamp2 promoter forward 5’-GGC AGG AGA AAG ACA CCA CTA TT-3’, reverse 5’- TTT AAG CCA AAG AGC GGA GGA G-3’.

Cells were washed in PBS and protein was extracted by incubating cells in lysis buffer (20 mM HEPES [pH7.7], 0.3 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 1% Triton X-100, 0.1% SDS with 1X Protease inhibitor cocktail [Roche] and Phosphatase inhibitor cocktail [Roche]) for 1 h on ice. 50 μg of protein extract was resolved in an 8% polyacrylamide-SDS gel, blotted to a PVDF membrane and incubated with antisera against STAMP2 (Medprobe, 1:1000), GRP78 (Cell signaling, cs3183) (1:600) ATF4 (Cell signaling, cs1185) (1:1000), CHOP (Cell signaling, cs2895) (1:1000), C/EBPα (Santa Cruz, sc-61 or Abcam, ab40764) (1:500), aP2/FABP4 (Abcam, ab81605) (1:1000), or β-Actin (Cell signaling, cs47778) (1:10000) in 5% BSA in TBS-0.1% Tween. Western images were obtained with a Kodak imaging station 4000R and the band intensities were quantified using Carestream Imaging Software.