Ciprofloxacin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Precirol® ATO 5 (Glyceryl distearate) was a generous gift sample from Gattefossé (Paramus, NJ, USA). Amicon® Ultra centrifugal filter devices with regenerated cellulose membrane (molecular weight cut off 100 kDa), Tween® 80, oleic acid, and Poloxamer 188 were acquired from Fischer Scientific (Hampton, NH, USA). Gellan gum was purchased from MP Biomedicals, LLC (Santa Ana, CA, USA). Other chemicals and glassware required for this research like HPLC grade solvents, scintillation vials, centrifuge tubes, HPLC vials were obtained from Fischer Scientific (Hampton, NH, USA). Whole eyes of male albino New Zealand rabbits were obtained from Pel-Freez Biologicals (Rogers, AR, USA).
Gellan gum
Manufactured by MP Biomedicals
Sourced in United States
Gellan gum is a high-molecular-weight, water-soluble polysaccharide produced by the bacterium Sphingomonas elodea. It is a linear anionic polymer composed of glucose, glucuronic acid, and rhamnose residues. Gellan gum is used as a thickening, stabilizing, and gelling agent in various applications, including food, pharmaceutical, and personal care products.
Automatically generated - may contain errors
Lab products found in correlation
Softworx software, by Cytiva (2 mentions)
Fm 1 43fx, by Thermo Fisher Scientific (2 mentions)
Analysis research acquisition software, by Olympus (2 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Hplc vials, by Thermo Fisher Scientific (1 mentions)
Poloxamer 188, by Thermo Fisher Scientific (1 mentions)
Hplc grade solvents, by Thermo Fisher Scientific (1 mentions)
Centrifuge tube, by Thermo Fisher Scientific (1 mentions)
Tween 80, by Thermo Fisher Scientific (1 mentions)
Oleic acid, by Thermo Fisher Scientific (1 mentions)
Scintillation vials, by Thermo Fisher Scientific (1 mentions)
Precirol ato 5, by Gattefosse (1 mentions)
Yeast extract, by Thermo Fisher Scientific (1 mentions)
3 protocols using gellan gum
1
Ciprofloxacin Ocular Drug Delivery
2
Gellan Gum Supported Microscopy Imaging
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For both the phase contrast and super-resolution microscopy cells were applied to gellan gum solidified nutrient media as described previously [30 (link)]. Briefly, cells from a liquid culture were spotted on nutrient media [10 g l−1 Tryptone (Oxoid: LP0042), 10 g l−1 NaCl (Oxoid: LP0005), 5 g l−1 Yeast extract (Oxoid: LP0021)] solidified with 8 g l−1 gellan gum (MP Biomedicals: 180106). The LB gellan gum (LBGG) nutrient media was supplemented with the fluorescent dye FM1-43FX (Life Technologies) to a final concentration of 5 μg ml−1 to visualize the cell membrane for super-resolution microscopy. Phase-contrast microscopy was performed using an Olympus IX71 inverted microscope fitted with an environmental chamber (Solent Scientific, Segensworth, UK) and AnalySIS Research acquisition software (Olympus Australia, Notting Hill, VIC, Australia). Super-resolution microscopy was performed using the Delta Vision OMX SR microscope using 3D-SIM mode. Live images were captured using a 1.42 numerical aperture 60× oil objective, standard filter set, a scientific CMOS 512×512 pixel 15-bit camera (PCO AG, Kelheim, Germany) and AquireSR software. Raw 3D-SIM images were obtained through section using a 125 nm Z-step size, which were then reconstructed using SoftWorX software (Applied Precision, GE Healthcare).
3
Microscopic Imaging of Bacterial Cells
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For both the phase contrast and super-resolution microscopy cells were applied to gellan gum solidified nutrient media as described previously (30) . Briefly, cells from a liquid culture were spotted on nutrient media (10 g L -1 Tryptone (Oxoid: LP0042), 10 g L -1 NaCl (Oxoid: LP0005), 5 g L -1 Yeast extract (Oxoid: LP0021)) solidified with 8 g L -1 gellan gum (MP Biomedicals: 180106). The LB gellan gum (LBGG) nutrient media was supplemented with the fluorescent dye FM1-43FX (Life Technologies) to a final concentration of 5 μg mL -1 to visualise the cell membrane for superresolution microscopy. Phase-contrast microscopy was performed using an Olympus IX71 inverted microscope fitted with an environmental chamber (Solent Scientific, Segensworth, UK) and AnalySIS Research acquisition software (Olympus Australia, Notting Hill, VIC, Australia). Superresolution microscopy was performed using the Delta Vision OMX SR microscope using 3D-SIM mode. Live images were captured using a 1.42 numerical aperture 60x oil objective, standard filter set, a scientific CMOS 512x512 pixel 15-bit camera (PCO AG, Kelheim, Germany) and AquireSR software. Raw 3D-SIM images were obtained through section using a 125-nm Z-step size, which were then reconstructed using SoftWorX software (Applied Precision, GE Healthcare).
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