Blood (2 × 20 mL) was obtained from the peripheral veins of patients before treatment with antibiotics was started. It is unknown whether patients had received antibiotics prior to hospitalization. Blood (10 mL) was added to each of two culture flasks containing resins for antibiotic neutralization. Blood cultures (BACTEC™ Plus Aerobic/F and BACTEC™ Plus Anaerobic/F culture vials; Becton-Dickinson, Franklin Lakes, NJ, USA) were obtained in accordance with current blood culture guidelines [29 (link)] and incubated in an automated blood culture system (BD BACTEC FX; Becton-Dickinson) for 5 days at 37 °C. Positive blood cultures were Gram-stained, streaked onto Columbia sheep blood agar, chocolate agar, MacConkey lactose agar, and Schaedler agar (Becton-Dickinson) for overnight incubation at 37 °C, and species identification was then carried out using matrix-assisted laser desorption ionization time-of-flight mass spectrometry on a VITEK®MS device (bioMérieux, Marcy l’Étoile, France).
Macconkey lactose agar
Manufactured by BD
Sourced in France
MacConkey lactose agar is a culture medium used for the isolation and differentiation of Gram-negative, lactose-fermenting bacteria, such as Escherichia coli, from clinical and environmental samples. It contains lactose as the fermentable carbohydrate, bile salts, and crystal violet, which inhibit the growth of Gram-positive bacteria.
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3 protocols using macconkey lactose agar
1
Blood Culture Isolation and Identification
2
Oral Infection Model of Citrobacter rodentium
3
Oral Infection Model of Citrobacter rodentium
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Citrobacter rodentium strain DBS120 (pCRP1::Tn5)(31 (link)) was grown in Lennox LB Broth overnight at 37°C. Mice were inoculated via oral gavage with 100 μl of bacteria (i.e., 1–3×106 CFU) in PBS, and deprived of food and water for 2 hrs after challenge. Fecal shedding of C. rodentium was determined every 3 days by plating feces from infected mice on MacConkey lactose agar (Becton Dickinson) supplemented with kanamycin (40 μg/ml). Pathogen levels were also determined is spleen homogenates plated in the same fashion as stool samples(7 (link)).
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