Ascorbic acid

Manufactured by Shimadzu

Sourced in India, Japan

Ascorbic acid is a chemical compound that is commonly used as a laboratory reagent. It is a white crystalline solid that is highly soluble in water and slightly soluble in organic solvents. Ascorbic acid is a reducing agent and is often used in analytical chemistry applications.

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Lab products found in correlation

C18 reverse phase column, by Phenomenex (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Acetone, by Chron Chemicals (1 mentions) 2 2 azinobis 3 ethyl benzothiazoline 6 sulfonic acid abts, by Sangon (1 mentions) β carotene, by Solarbio (1 mentions) Analytical grade ethanol, by Chron Chemicals (1 mentions) Acetic acid, by Chron Chemicals (1 mentions) Aluminum trichloride, by Chron Chemicals (1 mentions) Authentic ascorbic acid, by Solarbio (1 mentions) Quercetin, by Solarbio (1 mentions) Acetonitrile, by Tedia (1 mentions) Methyl alcohol, by Tedia (1 mentions)

2 protocols using ascorbic acid

HPLC Analysis of Ascorbic Acid

Cited 1 time

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All solvents used for analysis were of high performance liquid chromatography (HPLC) grade and those used for the extraction were reagent grade, procured from Merck life sciences Private Limited, Bengaluru, India. MilliQ plus (Millipore India Private Limited, Bengaluru, India) purified water was used for the experiments. The analytical standards of CAAS (CAS no: 5743-28-2 Sigma-Aldrich, Bangalore, India) and ascorbic acid (CAS no: 50-81-7) were procured from Sigma Aldrich, Bangalore, India. Food-grade calcium ascorbate 98% suitable for human supplementation (DSM Nutritional Products Limited, Heanor, UK) and the surface-modified liposomal particles of CAAS with fenugreek galactomannan (FC+) was received from Akay Natural Ingredients, Cochin, India along with a detailed certificate of analysis indicating food safety parameters. ascorbic acid content was estimated using a HPLC (Shimadzu SPD20AT) coupled with SPD-M20A prominence photodiode array detector (PDA) (Shimadzu India Private Limited, Mumbai, India) and a Phenomenex reverse phase C18 column (150 × 4.6 mm; 3 μm, 100 Å) following a previously reported validated procedure by Robitaille and Hoffer.^{28 (link)}

Joseph A., Kumar D., Balakrishnan A., Shanmughan P., Maliakel B, & IM K. (2021). Surface-engineered liposomal particles of calcium ascorbate with fenugreek galactomannan enhanced the oral bioavailability of ascorbic acid: a randomized, double-blinded, 3-sequence, crossover study. RSC Advances, 11(60), 38161-38171.

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Phytochemical Analysis of Plant Extracts

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Analytical-grade oxalic acid, potassium acetate, 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and FRAP working solution were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). Analytical-grade ethanol, acetone, petroleum, acetic acid, aluminum trichloride, and ferrous sulfate (FeSO₄·7H₂O) were purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). The standards of chlorophylls (a and b), carotenoids (neo-xanthin, violaxanthin, lutein, and β-carotene), authentic ascorbic acid, and quercetin were obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). HPLC-grade isopropanol, acetonitrile, and methyl alcohol were purchased from Tedia Company, Inc. (Fairfield, OH, USA). The spectrophotometer was purchased from Shanghai Mapada Instruments Co., Ltd. in China. High-performance liquid chromatography (HPLC) analysis of ascorbic acid and individual phenolic compounds was carried out using a Shimadzu LC-10AT system equipped with a UV detector (Shimadzu Scientific Instruments Inc., Kyoto, Japan). The mass spectrometer (Agilent 1100 SL, Santa Clara, CA, USA) was equipped with dual electrospray ionization (ESI) source. The freeze-dryer FDU-2110 was purchased from Tokyo Physicochemical Equipment Co., Ltd. (EYELA) in Tokyo, Japan.

Zhang Y., Huang W., Zhang C., Huang H., Yang S., Wang Y., Huang Z., Tang Y., Li X., Lian H., Li H., Zhang F, & Sun B. (2023). Variation in the Main Health-Promoting Compounds and Antioxidant Capacity of Three Leafy Vegetables in Southwest China. Molecules, 28(12), 4780.

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