Log In Sign up
The largest database of trusted experimental protocols

Syringe pump

Manufactured by New Era Pump Systems
Visit Supplier
Sourced in United States

The Syringe Pump is a laboratory instrument designed to accurately dispense and control the flow of liquids. It utilizes a stepper motor to precisely drive a syringe plunger, enabling the user to set specific flow rates and dispensing volumes. The Syringe Pump is a versatile tool commonly used in various research and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Micromanipulator, by World Precision Instruments (4 mentions) Chloroform, by Scharlab (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Lyoquest 85, by Telstar (1 mentions) Ix51 inverted microscope, by Olympus (1 mentions) Jsm 6390la, by JEOL (1 mentions) Sylgard 184 elastomer kit, by Dow (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions) Zen 2, by Zeiss (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) Prism 6, by GraphPad (1 mentions) Prism 8, by GraphPad (1 mentions) Tm3030plus, by Hitachi (1 mentions)

Close spelling variants of this product

Syringe Pump NE-300 NE-300 Just InfusionTM Syringe Pump NE-1000 Programmable Single Syringe Pump NE-1000 syringe pump Programmable syringe pump NE-4000 Programmable 2 Channel Syringe Pump Syringe Pump Pro software Multi-phaser NE-1000 syringe pump

32 protocols using syringe pump

1

Measuring Hollow Fiber Membrane Burst Pressure

Check if the same lab product or an alternative is used in the 5 most similar protocols
To test burst pressure, a syringe pump (New Era Pump Systems, Farmingdale, NY) with a 30-mL syringe was connected via Tygon® tubing to one end of the HFM, while the other end was connected to a pressure gauge (SSI Technologies, Janesville, WI). The pump was programmed to pump DI water at a rate of 1 mL/min until failure. The maximum pressure attained before failure was defined as the burst pressure.
Mercado-Pagán Á.E., Kang Y., Findlay M.W, & Yang Y. (2015). Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes. Materials science & engineering. C, Materials for biological applications, 49, 541-548.
+ Open protocol
+ Expand
2

Magnetic Microsphere Fabrication Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microspheres were produced via an oil/water (o/w) emulsion method with solvent evaporation. [19] (link) The aqueous phase consisted of a solution of polyvinylalcohol (PVA, Sigma-Aldrich) in MiliQ water (4% w/v) and the organic phase was the polymer dissolved in chloroform (Scharlau) (3% w/v). Ferrite nanoparticles (MNPs, EMG1300 Ferrotec Ferrofluids, nominal particle diameter: 10 nm) were added to the organic phase to confer magnetic properties on the resulting microspheres, MNPs were added at 5% w/w with respect to the polymer weight. 20 mL of the polymer solution was added using a syringe pump (New Era Pump Systems Inc, USA) with a flow rate of 1mL/min in 200 mL of aqueous phase under 150 rpm agitation (IKA Works Inc, Germany).
Fifteen minutes after the polymer incorporation 150 mL of miliQ water was added, after which the emulsion was stirred for 48 hours. The microspheres were then washed four times with MiliQ water by decanting the suspension, the water was removed and the microspheres were frozen gradually to -80ºC for subsequent lyophilization for 48 hours in a LyoQuest 85 (TELSTAR, Spain).
Clara-Trujillo S., Marín-Payá J.C., Cordón L., Sempere A., Gallego Ferrer G, & Gómez Ribelles J.L. (2019). Biomimetic microspheres for 3D mesenchymal stem cell culture and characterization. Colloids and surfaces. B, Biointerfaces, 177.
+ Open protocol
+ Expand
3

Saliva-based VP Isolation and Enumeration

Check if the same lab product or an alternative is used in the 5 most similar protocols
Saliva samples were hydrodynamically driven through the VP selection chip using a syringe pump (New Era Pump Systems Inc., Farmingdale, NY USA) and a 1-ml tuberculin syringe fitted with a capillary connector (Inner-Lok union capillary connectors; Polymicro Technologies) and barbed socket Luer Lock fittings (3/32″ ID, McMaster-Carr). Saliva samples were centrifuged at 1000g for 5 min to pellet buccal cells. For all saliva samples analyzed, none were diluted and no chip failure was noticed. Samples were infused into the device at varying volumetric flow rates (20 to 100 μl/min). Following sample introduction, the VP selection chip was rinsed with PBS at 50 μl/min. All buffer solutions were filtered through a 0.45-μm polypropylene filter (Thermo Fisher Scientific) before use. VPs were photoreleased in ~20 to 35 μl of PBS and enumerated using RT-qPCR.
Gamage S.S., Pahattuge T.N., Wijerathne H., Childers K., Vaidyanathan S., Athapattu U.S., Zhang L., Zhao Z., Hupert M.L., Muller R.M., Muller-Cohn J., Dickerson J., Dufek D., Geisbrecht B.V., Pathak H., Pessetto Z., Gan G.N., Choi J., Park S., Godwin A.K., Witek M.A, & Soper S.A. (2022). Microfluidic affinity selection of active SARS-CoV-2 virus particles. Science Advances, 8(39), eabn9665.
+ Open protocol
+ Expand
4

Electrospinning of PVA-Fish Oil Mats

Check if the same lab product or an alternative is used in the 5 most similar protocols
The electrospinning solutions were added to a syringe and placed in a syringe pump (New Era Pump Systems, Inc., USA). A 16 G needle (Proto Advantage, Canada) was used. The syringe pump delivered the PVA-emulsion blends with a flow rate of 0.02 ml/min. Using a high voltage power supply (Gamma High Voltage Research, USA), an electric field of 20 kV was applied between the spinneret of the syringe and a 5 × 5 cm collector plate made of stainless steel with alumina foil wrapped around it. The distance between the syringe tip and the collector plate was 10 cm. The electrospinning process was conducted at room temperature. From the 18 electrospinning solutions shown in Table 1, electrospun mats were obtained with a fish oil content ranging from 12.0 to 38.4% (w/w). Fibers with a final oil content of 12.2% (w/w) containing 5% (w/w) fish oil-in-water emulsions stabilized with WPI were produced in large quantity for peroxide value and secondary oxidation products determinations. For that purpose, fibers were produced in batches of 5 h. Fibers with 12.2% (w/w) oil containing 5% (w/w) fish oil-in-water emulsions stabilized with WPI were also produced for peroxide value determination from: i) a solution containing 13.5% (w/w) PVA, and ii) a solution containing 10.5% (w/w) PVA and 100 ppm of ethylenediaminetetraacetic acid (EDTA) in the parent emulsion.
Moreno P.J.G., Stephansen K., van der Kruijs J., Guadix A., Guadix E.M., Chronakis I.S, & Jacobsen C. (2016). Encapsulation of fish oil in nanofibers by emulsion electrospinning: Physical characterization and oxidative stability. Journal of Food Engineering., 183.
+ Open protocol
+ Expand
5

3D Bioprinting of Microfibrous Scaffolds

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the printing of a construct, a commercial 3D bioprinter (Cellink Inkredible, Gothenburg, Sweden) was used in along with a customized extrusion system using 27G blunt syringe needles (OD: 410 mm; ID: 210 mm). The needles were connected to a syringe pump (New Era Pump Systems Inc., Suffolk County, NY) for the injection of the ink through PVC tubing (ColePalmer). All the junctions were sealed via epoxy glue. The customized extrusion system was attached to the commercial printer printhead using a custom-designed L-shaped holder made out of poly(methyl methacrylate) (PMMA) sheets. For the deposition of the ink into desired structures, a MATLAB was used to automatically generate the G-code for the bioprinter. Specifically, microfibrous scaffolds were printed through the deposition of one single continuous microfiber shaped in 3D for each scaffold.
Mehrotra S., de Melo B.A., Hirano M., Keung W., Li R.A., Mandal B.B, & Shin S.R. (2020). Nonmulberry Silk Based Ink for Fabricating Mechanically Robust Cardiac Patches and Endothelialized Myocardium‐on‐a‐Chip Application. Advanced functional materials, 30(12), 1907436.
+ Open protocol
+ Expand
6

Electrochemical Flow-Cell Analyte Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Working electrodes were positioned in a custom electrochemical flow-cell using a micromanipulator (World Precision Instruments, Sarasota, FL). A syringe pump (New Era Pump Systems, Wantagh, NY) supplied a continuous buffer stream of 0.5 mL min−1 (1.0 mL min−1 for data in Figure 3) across the working and reference electrodes. Two-second bolus injections of analyte to the microelectrode surface were accomplished with a six-port HPLC valve mounted on a two-position actuator controlled by a digital pneumatic solenoid valve (Valco Instruments, Houston, TX). The entire apparatus was enclosed in a custom-built grounded Faraday cage.
Meunier C.J., Roberts J.G., McCarty G.S, & Sombers L.A. (2017). The Background Signal as an In Situ Predictor of Dopamine Oxidation Potential: Improving Interpretation of Fast-Scan Cyclic Voltammetry Data. ACS chemical neuroscience, 8(2), 411-419.
+ Open protocol
+ Expand
7

Fabrication of PNIPAM Nanofibers

Check if the same lab product or an alternative is used in the 5 most similar protocols
A vertical electrospinning set-up was utilized to fabricate PNIPAM nanofibers. Voltage was supplied by a DC power supply (Omega High Voltage Research, FL), and flow rate was controlled using a syringe pump (New Era Pump Systems, NY). All samples were collected on a grounded collector plate, wrapped in aluminum foil, and vacuum desiccated overnight before further use. A fractional factorial design of experiment (DOE) with three factors and two levels was constructed to identify electrospinning process parameters influencing PNIPAM fiber diameter. The factors and levels examined were: collecting distance (10 and 20cm), applied voltage (10 and 25 kV), and syringe pump flow rate (0.1 and 1.0 mL/hr). The PNIPAM solution was loaded into a 5 mL glass syringe, fitted with a 22-gauge (0.41mm ID) needle.
Young R.E., Graf J., Miserocchi I., Van Horn R.M., Gordon M.B., Anderson C.R, & Sefcik L.S. (2019). Optimizing the alignment of thermoresponsive poly(N-isopropyl acrylamide) electrospun nanofibers for tissue engineering applications: A factorial design of experiments approach. PLoS ONE, 14(7), e0219254.
+ Open protocol
+ Expand
8

Collagen Microsphere Fabrication and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Type I collagen (5 mg/ml) isolated from bovine Achilles tendon was used to fabricate the collagen microspheres. Either the collagen solution alone or collagen solution mixed with poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG) (0.01 mM and 0.1 mM) was transferred into a syringe with a needle (25 G1, BD Biosciences, Franklin Lakes, NJ). The collagen solution was injected directly into the cell culture medium on a cell culture plate at the rate of either 0.1 ml/min or 0.4 ml/min using a syringe pump (New Era Pump Systems, Inc., Farmingdale, NY). The cell culture plate was then transferred to an incubator (37°C, 5% CO2). Images of the microspheres at various time points (4 h, day 3, day 6, and day 10) after fabrication were taken using a microscope (Olympus IX51 Inverted Microscope, Center Valley, PA), and the diameters of the microspheres were measured and quantified.
Berndt M., Li Y., Seyedhassantehrani N, & Yao L. (2016). Fabrication and characterization of microspheres encapsulating astrocytes for neural regeneration. ACS biomaterials science & engineering, 3(7), 1313-1321.
+ Open protocol
+ Expand
9

Electrochemical Analysis with Flow Cell

Check if the same lab product or an alternative is used in the 5 most similar protocols
All flow cell experiments were conducted in a grounded Faraday cage. The electrochemical system was comprised of a CFM working electrode backfilled with 0.5 M potassium acetate and a chlorided Ag/AgCl wire as a reference electrode. Potentials were applied to the working electrode using a ChemClamp potentiostat and headstage (Dagan, Minneapolis, MN). A syringe pump (New Era Pump Systems, Farmingdale, NY) was used to push aCSF through a six-port loop injector (Valco Instruments, Houston, TX) at a flow rate of 2 mL/min. The CFM tip was submerged in solution at the flow cell output. Data were collected and analyzed using TarHeel (Department of Chemistry, University of North Carolina at Chapel Hill) and Knowmad (Knowmad Technologies, LLC, Tuscon, AZ) software. All data were collected at a frequency of 10 Hz and low pass filtered at 3 kHz.
Stucky C, & Johnson M.A. (2022). Improved Serotonin Measurement with Fast-Scan Cyclic Voltammetry: Mitigating Fouling by SSRIs. Journal of the Electrochemical Society, 169(4), 045501.
+ Open protocol
+ Expand
10

In Vitro Electrochemical Glucose Oxidase Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
All in vitro data were collected at room temperature in a flow-injection apparatus using commercially available HDCV software (University of North Carolina at Chapel Hill, Department of Chemistry, Electronics Facility). A micromanipulator (World Precision Instruments Inc., Sarasota, FL) was used to position the GOx EME in a custom built electrochemical cell with a continuous flow of PBS (1 mL/min) supplied by a syringe pump (New Era Pump Systems, Inc., Wantagh, NY). Two-sec bolus injections of analyte were introduced to the GOx EME surface with a 6-port HPLC valve mounted on a two-position air actuator controlled by a digital pneumonic solenoid valve (Valco Instruments Co., Inc., Houston, TX). Triangular voltammetric waveforms were applied at 400 V/s and at a frequency of 10 Hz.
Smith S.K., Lee C.A., Dausch M.E., Horman B.M., Patisaul H.B., McCarty G.S, & Sombers L.A. (2017). Simultaneous Voltammetric Measurements of Glucose and Dopamine Demonstrate the Coupling of Glucose Availability with Increased Metabolic Demand in the Rat Striatum. ACS chemical neuroscience, 8(2), 272-280.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!