Protein extracts were resolved by SDS-PAGE and indicated primary antibodies were used. ABCB1 antibody (SC-8313, rabbit-polyclonal, 1:500 dilution) was purchased from Santa Cruz Biotechnology. AR-441 antibody (SC-7305, mouse-monoclonal antibody, 1:1000 dilution) was purchased from Santa Cruz Biotechnology. Tubulin (T5168, mouse monoclonal antibody, 1:6000 dilution) was purchased from Sigma-Aldrich. GAPDH (MAB374, mouse monoclonal antibody, 1:10000) was purchased from EMD Millipore. Tubulin or GAPDH was used to monitor the amounts of samples applied. Proteins were visualized with a chemiluminescence detection system (Cat#: WBLUR0500) purchased from Millipore.
Ar 441 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The AR-441 antibody is a laboratory reagent produced by Santa Cruz Biotechnology. The antibody specifically recognizes the androgen receptor (AR) protein, which plays a crucial role in the regulation of gene expression related to male sexual development and function. The AR-441 antibody can be used in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and distribution of the androgen receptor in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Abcb1 antibody, by Santa Cruz Biotechnology (2 mentions)
Gapdh, by Merck Group (1 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Polylysine, by Merck Group (1 mentions)
Anti α tubulin antibody, by Merck Group (1 mentions)
Gapdh antibody d16h11, by Cell Signaling Technology (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
5 protocols using ar 441 antibody
1
Western Blot Protein Analysis
2
Subcellular Localization of Androgen Receptor Variants
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COS-7 cells were transfected with AR-FL, AR-Q784*, or AR-K823*. NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific Cat. # 78833) were used to extract nuclear and cytoplasmic proteins following manufacturer's protocol. Immunoblot analysis was then performed as described above. For immunofluorescence staining, cells were fixed with methanol and then incubated with AR-441 antibody (Santa Cruz), followed by incubation with fluorescence labeled secondary antibodies (Alexa Fluor 488, Molecular Probes). Micrographs were taken under EVOS auto fluorescence microscope (ThermoFisher) at the same microscope settings.
3
Western Blot Analysis of Protein Targets
4
Prostate Cancer Cell Line Culturing and Transfection
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The PCa cell lines LNCaP, 22Rv1, and LAPC-4 were cultured in RPMI 1640 (Media Tech/Cellgro) supplemented with 10% fetal bovine serum (FBS) and 2 mM l -glutamine. LNCaP and LAPC-4 cells were cultured on dishes or plates coated with PBS containing 0.01% poly-lysine (Sigma-Aldrich, St. Louis, MO, USA) to facilitate cell growth. All cell line cultures were maintained at 37°C in a humidified 5% CO2 incubator.
One day prior to transfection, cells were seeded at 60% confluence in RPMI 1640 containing 10% FBS. The SSOs were delivered at a final concentration of 5–20 nM using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations. Cells were harvested 48 h after SSO transfection for western blotting and qRT-PCR.
A list of all SSO sequences screened in this work is available upon request.
The primary antibodies used in this work were as follows: AR antibody (441) from Santa Cruz Biotechnology, GAPDH antibody (D16H11) from Cell Signaling Technology (Boston, MA, USA), and anti-α-tubulin antibody from Sigma-Aldrich (St. Louis, MO, USA). Secondary antibodies against rabbit (NA934) and mouse (NA931) primary antibodies were purchased from GE Healthcare.
One day prior to transfection, cells were seeded at 60% confluence in RPMI 1640 containing 10% FBS. The SSOs were delivered at a final concentration of 5–20 nM using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations. Cells were harvested 48 h after SSO transfection for western blotting and qRT-PCR.
A list of all SSO sequences screened in this work is available upon request.
The primary antibodies used in this work were as follows: AR antibody (441) from Santa Cruz Biotechnology, GAPDH antibody (D16H11) from Cell Signaling Technology (Boston, MA, USA), and anti-α-tubulin antibody from Sigma-Aldrich (St. Louis, MO, USA). Secondary antibodies against rabbit (NA934) and mouse (NA931) primary antibodies were purchased from GE Healthcare.
5
Analyzing Androgen Receptor Interactions in LNCaP Cells
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