Esi compass 1.3 for hct esquire

The HPLC system (1100/1200 series, Agilent, Waldbronn, Germany) consisted of a binary pump (G1312A), an autosampler (G1329B), and a DAD-detector (G1316A). It was coupled to a HCT Ultra Ion Trap mass spectrometer (Bruker Daltonics, Bremen, Germany) with an electrospray ionization source (ESI). The anthocyanins and copigments were separated on a Luna C18(2) 3 μm column (150 × 2.0 mm, Phenomenex, Aschaffenburg, Germany) using water/acetonitrile/formic acid 95:3:2 v/v/v (eluent A) and water/acetonitrile/formic acid 48:50:2 v/v/v (eluent B) at a flow rate of 200 μL/min. Gradient elution was performed, starting with 6% eluent B and rising to 35% over 30 min. The level of eluent B was then set to 40% until minute 35 and then 90% until 45 min. This level was maintained for 5 min before being reduced to 30% until 55 min. Finally, the initial conditions (6% eluent B) were restored until 70 min. The ESI source was operated in positive mode (anthocyanins), negative mode (copigments) and alternating mode (+/− 3000 V), using nitrogen as the nebulizer gas (60 psi) and the drying gas (11 L/min, 330 °C). The sample extracts were dissolved in 2 mL eluent A. An aliquot of 5 μL was analyzed by the HPLC/DAD/ESI-MSⁿ method described above using the Bruker Hystar V.3.2, Bruker ESI-Compass 1.3 for HCT/Esquire, and Data Analysis Version 3.0 software packages (Bruker Daltonics, Bremen, Germany).

The HPLC system (1100/1200 series, Agilent, Waldbronn, Germany) includes a binary pump (G1312A), an autosampler (G1329B), and a DAD-detector (G1316A). It was coupled to an HCT Ultra Ion Trap mass spectrometer (Bruker Daltonics, Bremen, Germany) with an electrospray ionization source (ESI). The anthocyanins and copigments were separated on a Luna C18(2) 3 µ column (150 × 2.0 mm, Phenomenex (Torrance, CA, USA)) using water/acetonitrile/formic acid (95/3/2; v/v/v) (eluent A) and water/acetonitrile/formic acid (48/50/2; v/v/v) (eluent B) at a flow rate of 0.2 mL/min. Gradient elution was performed: 0 min 6% B, 30 min 35% B, 35 min 40% B, 45 min 90% B, 50 min 90% B, 55 min 30% B, 70 min 6% B. The ESI source was operated in alternating mode (+/−3000 V) (positive mode for anthocyanins and negative mode for copigments), using nitrogen as the nebulizer (50 psi) and drying gas (10 L/min, 365 °C). The sample extracts (around 2 mg) were dissolved in 2 mL of eluent A with a pH of 2.52 so that the flavylium cation form of the anthocyanins was stabilized. Aliquots of 5 µL of each sample were analyzed by the HPLC-PDA-ESI-MS/MS method described above according to Ostberg-Potthoff et al. [33 (link)] using the Bruker Hystar 3.2, Bruker ESICompass 1.3 for HCT/Esquire, and Data Analysis Version 3.0 software packages (Bruker Daltonics, Bremen, Germany).