Anti mir 145 5p

The overexpression vector of circSCAF11 (pcDNA-circSCAF11), miR-145-5p mimic, small interfering RNA targeting circSCAF11 (si-circSCAF11), miR-145-5p inhibitor (anti-miR-145-5p) and control groups (pcDNA-NC, miR-NC mimic, si-NC and anti-miR-NC) were amplified by Ribobio Co., Ltd. (Guangzhou, China). PcDNA-circSCAF11 and pcDNA-NC were transfected into cells to determine the effects of circSCAF11 overexpression on ropivacaine-mediated glioma growth in vitro and in vivo. Si-circSCAF11 and anti-miR-145-5p were transfected into cells with their controls to illustrate that whether circSCAF11 regulated the glioma process by sponging miR-145-5p. T98G and LN229 cells were transfected with Lipofectamine 2000 (Thermo Fisher). The primer sequences were pcDNA-circSCAF11 5ʹ-CGGAATTCTAATACTTTCAGGAAACTCTTTATACAGTGAAACAGG-3ʹ (forward), and 5ʹ-CGGAATTCTAATACTTTCAGGAAACTCTTTATACAGTGAAACAGG-3ʹ (reverse); miR-145-5p mimic 5ʹ-GUCCAGUUUUCCCAGGAAUCCCU-3ʹ; miR-NC mimic 5ʹ-UUUGUACUACACAAAAGUACUG-3ʹ; miR-145-5p inhibitor 5ʹ-AGGGAUUCCUGGGAAAACUGGAC-3ʹ; anti-miR-NC 5ʹ-CAGUACUUUUGUGUAGUACAAA-3ʹ; si-circSCAF11 5ʹ-GCTGACAGATGCCCAATAT-3ʹ and si-NC 5ʹ-GCTAGACCCGTAACAGTAT-3ʹ.