Anti strap 18277 1 ap

The following primary antibodies were used: The mouse antiSIRT7 (sc-365344), rabbit anti-SIRT7 (sc135055), anti-p53 (FL) (sc-6243), anti-p53 (DO-1) (sc-126), anti-p21 (sc-271532), anti-actin (sc-8432) and anti-tubulin (sc-73242) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-STRAP (18277-1-AP) was purchased from Proteintech (Wuhan, China), the anti-GAPDH (#5174S) was purchased from Cell Signaling Technology (Danvers, MA, US), the anti-FLAG (F3165) was purchased from Sigma–Aldrich (St. Louis, MO, US), the anti-HA (#26183) was purchased from Pierce (Rockford, Illinois, US), the anti-pan-acetyllysine (#9441L) was purchased from Cell Signaling Technology (Danvers, MA, US).pcDNA3.1-Flag/HA-STRAP/SIRT7 was obtained by subcloning STRAP/SIRT7 sequence into pcDNA3.1-Flag/HA vector. The mutant constructs were generated by PCR amplification of STRAP/SIRT7 with specific primers. STRAP full-length (FL), STRAP (ΔC1, ΔC3, ΔC4, ΔC5, ΔC6, WD3, WD4, WD6, WD7, CT) were subcloned into the pGEX4T-3 vector.
STRAP siRNA 1#: gggugcaacacugaauaag; siRNA 2#: uuacgcauauaugacuuga (Genepharma, Suzhou, China).

Trypan blue stain was obtained from Life Technologies Corporation (Grand Island, NY, USA). Primary antibodies used for Western blotting included the following: polyclonal rabbit anti-STRAP (18277-1-AP) from Proteintech (Rosemont, IL, USA), monoclonal rabbit anti-PGDFRβ (#3169) and monoclonal rabbit anti-vinculin (E1E9V, #13901) from Cell Signaling (Danvers, MA, USA), and monoclonal mouse anti-FLAG (F3165) and monoclonal mouse anti-β-actin (A1978) from Sigma Aldrich. Antibodies were used according to manufacturers’ suggestions.