Snu c4

All colorectal cancer cells (DLD-1, HT29, ANU-C2B, SNU-C4, SNU-C5, Colo320 DM)
were purchased from the Korean Cell Line Bank and maintained in RPMI-1640 Medium
(Invitrogen) containing 10% fetal bovine serum with 5% CO₂ in a
37℃ incubator. miR199a5p mimics and negative mimics were purchased from
Dharmacon. Cells were transfected with a miR-199a-5p mimic using the DharmaFECT
1 transfection reagent (Dharmacon) according to the manufacturer’s
protocol. The negative mimic was used for control purposes. After 72 h of
incubation, cells were harvested and used for extraction of total RNA or
protein.

A human HCC cell line, Hep3B, a human HB line, HepG2, and five human CRC cell lines, LS174T, HCT116, DLD‐1, SW480, and RKO were purchased from the American Type Culture Collection (Manassas, VA). Additionally, three human HCC cell lines, Alexander, HuH‐7, and HLE, and a human HB cell line, HuH‐6, were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan), and a CRC line, SNU‐C4, was obtained from the Korean Cell Line Bank (Seoul, Korea). All cells were cultured in appropriate media supplemented with 10% FBS (BioSera, Boussens, France) and antibiotic/antimycotic solution (Fujifilm Wako Pure Chemical, Osaka, Japan). SW480 cells were maintained without CO₂ supplementation at 37°C and others cells were maintained in 5% CO₂ at 37°C. For the inhibition of DNA methylation, cells were treated with 5‐azacytidine (Fujifilm Wako Pure Chemical) at a concentration of 1 μM for 48 h.