Immunohistochemical staining for CD4+ T cell, CD68+ macrophages as APCs and tubular Kim-1 and TLR4 was performed on 6-μm periodate lysine paraformaldehyde-fixed sections [21 (link)]. CD11c+ and F4/80+ cells as APCs (DCs, CD11c+ and pan-macrophages, F4/80+ cells) were identified in 4-ìm-thick formalin-fixed sections. The numbers of these cells were assessed in 10 fields per slide at ×400 magnification, and the results are expressed as cells per high-power field (c/hpf). The primary monoclonal antibodies used were rat monoclonal antibody GK1.5 for CD4+ T cells (Pharmingen, San Diego, CA, USA), for APCs with CD68+ (Serotec, Oxford, U.K.), F4/80 hybridoma culture supernatant (HB 198; American Type Culture Collection, Manassas, MD, USA) and mouse monoclonal antibody for CD11c+ (Abcam, Cambridge, U.K.), rabbit polyclonal antibody TLR4 (Novus Biologicals, Littleton, CO, USA) and rat monoclonal antibody Tim-1 (R & D Systems, Minneapolis, MN, USA).
Tim 1
Manufactured by R&D Systems
Sourced in United States
The Tim-1 is a laboratory equipment designed for use in biological and biochemical research. It functions as a precision temperature-controlled incubator, capable of maintaining consistent temperature conditions for samples or experiments.
Automatically generated - may contain errors
Lab products found in correlation
F4 80 hybridoma culture supernatant hb 198, by American Type Culture Collection (2 mentions)
Gk1.5 for cd4 t cells, by BD (2 mentions)
Tim 3, by R&D Systems (2 mentions)
Fibronectin, by Merck Group (1 mentions)
Ecl reagent, by PerkinElmer (1 mentions)
β actin, by Merck Group (1 mentions)
Amersham imager, by Cytiva (1 mentions)
E selectin, by R&D Systems (1 mentions)
Cd162, by BD (1 mentions)
Cx3cl, by R&D Systems (1 mentions)
Fibronectin, by R&D Systems (1 mentions)
Igg2b, by R&D Systems (1 mentions)
6 protocols using tim 1
1
Immunohistochemical Profiling of Immune Cells
2
Glycoproteomic Studies of TIM Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinantly expressed TIM-1 (AAC39862) and TIM-4 (Q96H15) for glycoproteomic studies were purchased from R&D Systems (9319-TM, 9407-TM), whereas recombinant extracellular protein for functional studies was made in house (see below). For structural characterization, TIM-1 was purchased from R&D systems (11157-TM) and TIM-4 was purchased from LifeSpan Biosciences (LS-G139224). TIM-3 (Q8TDQ0) was purchased from LifeSpan Biosciences (LS-G97947). CD43 (P16150) and TIM-1 recombinantly expressed in NS0 cells were purchased from R&D systems (9680-CD,1750-TM). C1-Inh (P05155) and fibronectin (P02751) isolated from human plasma were purchased from Sigma Aldrich (E0518, F1056). Bovine Fetuin-A (P12763) was purchased from Promega (V4961). GP1bɑ (P07359) was isolated as described previously8 . The plasmids for His-tagged pET28a-SmEnhancin and recombinant StcE protein were kindly provided by the Bertozzi laboratory.
3
Western Blot Analysis of Tim-1 and Tim-3
4
Recombinant Proteins and Antibodies for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant proteins and antibodies. Human glycosylated recombinant proteins: C1-INH, CX3CL, CD44, CD45, CD55, CD97, CD99, CD162, TIM1, TIM3, TIM4, fibronectin, E-selectin and human Integrin alpha M beta 2 were obtained from R&D systems Inc. (Minneapolis, MN 55413), while the human glycosylated recombinant proteins: CD34, CD43, CD93, and CD164 were obtained from Sino Biologicals (Beijing 100176 P. R. China). Antibodies used in this study were dye conjugated mouse anti human CD3, CD4, CD8, CD14, CD16, CD56, CD43, CD44, CD45 and CD162 from BD and Invitrogen). Rabbit anti-sialic acid antibody was purchased from Cloud Clone Corp, Houston, TX.
5
Efferocytic Receptor Modulation in Gingiva
6
Immunohistochemical Analysis of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemical staining for CD4+ T-cells, Gr-1+ cells as neutrophils, and tubular Kim-1 (kidney injury molecule-1) was performed on 6 μm periodate lysine paraformaldehyde-fixed sections [36 ]. Here, CD11c+ cells and F4/80+ cells were examined as antigen-presenting cells (APCs; DCs, CD11c+ and pan-macrophages, F4/80+ cells), and phospho c-Jun+ cells were identified in 4 μm-thick formalin-fixed sections. The numbers of these cells were assessed in 10 fields per slide at ×400 magnification, and the results are expressed as cells per high-power field (c/hpf). The primary monoclonal antibodies used were rat monoclonal antibody GK1.5 for CD4+ T-cells (Pharmingen, San Diego, CA, USA), F4/80 hybridoma culture supernatant (HB 198; American Type Culture Collection, Manassas, MD, USA), mouse monoclonal antibody for CD11c+ cells (Abcam, Cambridge, U.K.), RB6–8C5 for neutrophils (anti-Gr-1; DNAX, Palo Alto, CA), and rat monoclonal antibody Tim-1 (R&D Systems, Minneapolis, MN, USA). Isotype-matched irrelevant monoclonal antibodies were used as controls.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!