Human ge 4 44k v2 microarray

Manufactured by Agilent Technologies

The Human GE 4x44K v2 microarray is a product designed for gene expression analysis. It provides a platform to measure the expression levels of a large number of human genes simultaneously.

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Lab products found in correlation

Accupower, by Bioneer (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Low input quick amp labeling kit, by Agilent Technologies (1 mentions) Agilent scanner g2565ba, by Agilent Technologies (1 mentions) Feature extraction software v10, by Agilent Technologies (1 mentions) Genespring gx 10, by Agilent Technologies (1 mentions)

4 protocols using human ge 4 44k v2 microarray

Transcriptomic Profiling of miRNAs

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Total RNAs were extracted using Trizol reagent (Ambion, Carlsbab, CA, USA), and cDNA was synthesized by AccuPower reverse transcriptase premix (BIONEER, Daejeon, Korea). Three RNA samples were used for miRNA microarray analysis. The RNA samples were then labeled with Cyanine 3-CTP (Cy3) and Cyanine 5-CTP (Cy5) in an in vitro transcription reaction using a Low Input Quick Amp Labeling kit (Agilent Technologies, Santa Clara, CA, USA), in accordance with the manufacturer's protocol. Labeled cRNA was hybridized on Human GE 4×44K v2 microarray (ID G2519-026652, Agilent Technologies), followed by manual washing, according to the manufacturer's procedures. The array was scanned using the Agilent DNA MicroArray Scanner and probe signals were quantified using Agilent's Feature Extraction ver. 10.10.1.1 (Agilent Technologies). Normalized data were analyzed using Subio platform v1.16.4376 (Subio Inc., Kagoshima, Japan).

Go Y.Y., Park M.K., Kwon J.Y., Seo Y.R., Chae S.W, & Song J.J. (2015). Microarray Analysis of Gene Expression Alteration in Human Middle Ear Epithelial Cells Induced by Asian Sand Dust. Clinical and Experimental Otorhinolaryngology, 8(4), 345-353.

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One-color Microarray Gene Expression Protocol

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After addition of spike-in controls, 200 ng of RNA was amplified and labeled using one-color microarray based gene expression protocol v 6.0; labeled cRNAs were hybridized to Human GE 4×44K v2 Microarray (Agilent Technologies, Santa Clara, CA). Slides were scanned by the Agilent G2565BA scanner and images were processed by Feature Extraction software v.10 (Agilent Technologies). The raw data were imported into GeneSpring 12.1 software (Agilent Technologies) and a quantile normalization was applied.
Microarray data have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus and are accessible via: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=xxsxlqouuaasmzk&acc=GSE44099.

Monticone M., Taherian R., Stigliani S., Carra E., Monteghirfo S., Longo L., Daga A., Dono M., Zupo S., Giaretti W, & Castagnola P. (2014). NAC, Tiron and Trolox Impair Survival of Cell Cultures Containing Glioblastoma Tumorigenic Initiating Cells by Inhibition of Cell Cycle Progression. PLoS ONE, 9(2), e90085.

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Transcriptome Analysis of KIRC Tissues

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Expression levels of mRNAs in 6 KIRC tissues and 6 paired normal adjacent tissues were detected on a Human GE 4×44 K v2 Microarray (G2519F-026652, Agilent Technologies). The clinical information of 6 KIRC species for gene chip analysis was provided in Supplementary Table 1. Flow chart of the gene chip experimental design was shown in Supplementary Fig. 1. In order to reduce the error caused by individual differences in patients, we mixed total RNA in pairs to detect. RNA samples were of high quality and reliability (Supplementary Table 2) for gene chip analysis (Human GE 4×44 K v2 Microarray). Agilent Technologies, Inc. was commissioned to perform labeling, array hybridization and scanning according to the standard protocol.

He Y., Wang X., Lu W., Zhang D., Huang L., Luo Y., Xiong L., Li H., Zhang P., Li Q, & Liang S. (2022). PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis. Cell Death & Disease, 13(2), 118.

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Microarray Analysis of CITED2 Knockdown in MDA-MB-231 Cells

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cDNA expression between scramble and shCITED2 MDA-MB-231 cells was compared using Agilent Human GE 4×44K v2 microarray (G4845A). Log2 transformed signal intensities, without background subtraction were imported into GeneSpring GX 10 software (Agilent Technologies) and (quantile) normalized within the sample type. Differentially expressed genes in shCITED2-expressing cells relative to scramble cells were identified based on ≥ two-fold change in gene expression. Quality assessment of samples and microarray analysis were conducted at the Sidney Kimmel Cancer Center Microarray Core Facility at Johns Hopkins University School of Medicine, Baltimore, MD (supported by NIH grant P30 CA006973 entitled Regional Oncology Research Center). Microarray data are deposited in the Array Express database www.ebi.ac.uk/arrayexpress under accession number E-MTAB-4267.

Jayaraman S., Doucet M., Lau W.M, & Kominsky S.L. (2016). CITED2 Modulates Breast Cancer Metastatic Ability Through Effects on IKKα. Molecular cancer research : MCR, 14(8), 730-739.

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