Lc 40ad

Following HPLC systems were used: Agilent HPLC 1200 (Santa Clara, CA, USA) and Shimadzu LC-40AD (Kioto, Japan). Chromatographic separation was performed on a Waters Symmetry C18 column, 5 µm, 250 mm × 4.6 mm. Chromatographic data were recorded and processed using Chem Station and LC solution software.

The HPLC samples were washed using refrigerated CentriVap concentrators purchased from Labconco, Kansas City, MO, USA. The Shimadzu, Kyoto, Japan, UPLC-MS/MS system used for the HPLC studies consisted of an autosampler (SIL-40AC), two solvent feed pumps with a gradient system (LC-40AD), a degasser (DGU- 30A5), a column oven (CTO-40AC), a UV detector (SPD-M20A), and a triple quadrupole mass spectrometer detector (model: LCMS-8045).
A model KJO-4282 Guard Cartridge System and a Lux Cellulose-2 (LC-2) chiral column with a cellulose tris(3-chloro-4-methylphenylcarbamate) stationary phase, as well as a Lux Cellulose-3 (LC-3) chiral column with a cellulose tris(4-methyl benzoate) from Phenomenex Co., Torrance, CA, USA, were used in the chiral chromatographic separations.
All incubations were performed in a dedicated incubator, model Incubators 1000 and Unimax 1010. The incubators were purchased from Heidolph, Schwabach, Germany with controlled temperature and rotation (250 RPM). Each piece of glass used was dried in an oven overnight before being cooled with a stream of nitrogen.