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The CP-A cells are a cell line derived from a human esophageal epithelial cell. They serve as a standard model for studying esophageal biology and disease.

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Lab products found in correlation

Het 1a cell, by American Type Culture Collection (2 mentions) Cp c cells, by American Type Culture Collection (2 mentions) Hek293ft, by Thermo Fisher Scientific (1 mentions) Sk gt 4, by Thermo Fisher Scientific (1 mentions)

3 protocols using cp a cells

1

Esophageal Cancer Cell Lines Protocol

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NCI-SB-EsC1 (EsC1) and NCI-SB-EsC2 (EsC2) were established in our lab from two esophageal cancer patients following chemo-radiation and definitive surgery for Barrett’s associated adenocarcinoma. OE19 and OE-33 esophageal adenocarcinoma lines were purchased from Sigma. Het-1A cells from normal esophageal epithelium immortalized with SV-40, as well as CP-A cells from non-dysplastic Barrett’s metaplasia and CP-C cells from high-grade Barrett’s dysplasia immortalized with hTERT were purchased from ATCC and were cultured as instructed. Full details of culture and smoke exposure conditions are submitted as Supplementary Materials.
Xi S., Inchauste S., Guo H., Shan J., Xiao Z., Xu H., Miettenen M., Zhang M.R., Hong J.A., Raiji M.T., Altorki N.K., Casson A.G., Beer D.G., Robles A.I., Bowman E.D., Harris C.C., Steinberg S.M, & Schrump D.S. (2015). Cigarette Smoke Mediates Epigenetic Repression of miR-217 During Esophageal Adenocarcinogenesis. Oncogene, 34(44), 5548-5559.
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2

Cell Line Authentication and Mycoplasma Screening

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Cell lines were obtained as follows: Cos7 and CP-A cells (ATCC), HEK-293FT (Life Technologies), FLO-1 and SKGT4 (kindly provided by Dr. Navtej Buttar, Mayo Clinic), JH-EsoAd1 (kindly provided by Drs. James Eshleman and Anirban Maitra, Johns Hopkins University School of Medicine), OE19 and OE33 (Sigma). All cell lines were obtained in 2014 and tested for authenticity using STR genotyping, and were screened periodically for mycoplasma contamination.
Blum A.E., Venkitachalam S., Guo Y., Kieber-Emmons A.M., Ravi L., Chandar A.K., Iyer P.G., Canto M.I., Wang J.S., Shaheen N.J., Barnholtz-Sloan J.S., Markowitz S.D., Willis J.E., Shyr Y., Chak A., Varadan V, & Guda K. (2016). RNA Sequencing Identifies Transcriptionally-Viable Gene Fusions in Esophageal Adenocarcinomas. Cancer research, 76(19), 5628-5633.
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3

Esophageal Cancer Cell Lines Protocol

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NCI-SB-EsC1 (EsC1) and NCI-SB-EsC2 (EsC2) were established in our lab from two esophageal cancer patients following chemo-radiation and definitive surgery for Barrett’s associated adenocarcinoma. OE19 and OE-33 esophageal adenocarcinoma lines were purchased from Sigma. Het-1A cells from normal esophageal epithelium immortalized with SV-40, as well as CP-A cells from non-dysplastic Barrett’s metaplasia and CP-C cells from high-grade Barrett’s dysplasia immortalized with hTERT were purchased from ATCC and were cultured as instructed. Full details of culture and smoke exposure conditions are submitted as Supplementary Materials.
Xi S., Inchauste S., Guo H., Shan J., Xiao Z., Xu H., Miettenen M., Zhang M.R., Hong J.A., Raiji M.T., Altorki N.K., Casson A.G., Beer D.G., Robles A.I., Bowman E.D., Harris C.C., Steinberg S.M, & Schrump D.S. (2015). Cigarette Smoke Mediates Epigenetic Repression of miR-217 During Esophageal Adenocarcinogenesis. Oncogene, 34(44), 5548-5559.
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