Gb11130

Manufactured by Wuhan Servicebio Technology

Sourced in China

The GB11130 is a laboratory equipment designed for cryogenic storage. It is capable of storing samples at extremely low temperatures to preserve their integrity and stability.

Automatically generated - may contain errors

Lab products found in correlation

Gb11132, by Wuhan Servicebio Technology (2 mentions) Protease inhibitor cocktail, by Wuhan Servicebio Technology (1 mentions) Ab652, by Abcam (1 mentions) Ab22759, by Abcam (1 mentions) Alphaeasefc, by Bio-Techne (1 mentions) Western blotting detection system, by Bio-Techne (1 mentions) G0002, by Wuhan Servicebio Technology (1 mentions) Gb11022 3, by Wuhan Servicebio Technology (1 mentions) Gb13044, by Wuhan Servicebio Technology (1 mentions) Gb11179, by Wuhan Servicebio Technology (1 mentions) Diaminobenzidine k5007, by Agilent Technologies (1 mentions) Gb13028, by Wuhan Servicebio Technology (1 mentions) Ab133737, by Abcam (1 mentions) Ab221675, by Abcam (1 mentions) Af5131, by Affinity Biosciences (1 mentions) Gb111141, by Wuhan Servicebio Technology (1 mentions) Af7013, by Affinity Biosciences (1 mentions) Df7011, by Affinity Biosciences (1 mentions) N cadherin, by Affinity Biosciences (1 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)

7 protocols using gb11130

Histological and Immunohistochemical Analysis of Aortic Tissue

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formaldehyde-fixed paraffin sections were cut in cross section (5 μm) and then stained with hematoxylin and eosin (HE) for analysis of structural integrity, Verhoeff's Van Gieson (EVG) staining for corruption of elastin, and Mac-3 for infiltration of macrophages.
The severity of elastin degradation is semiquantified, as previously described (grade 1, no degradation; grade 2, mild degradation; grade 3, severe degradation; and grade 4, presence of aortic rupture) [12 (link)].
Briefly, the aortic sections were deparaffinized and hydrated in H₂O and then incubated with primary antibodies against Mac-3 (1 : 100 dilution, BD, 550292), MCP-1 (1 : 500 dilution, Servicebio, G11199), VCAM-1 (1 : 200 dilution, Servicebio, G13336), MMP-2 (1 : 6000 dilution, Servicebio, GB11130), and MMP-9 (1 : 1000 dilution, Servicebio, GB11132) at 4°C overnight, followed by incubation with the corresponding secondary antibody for 1 h at 37°C. Then, the sections were dehydrated and images were obtained by a Nikon microscope equipped with a CCD camera. Immunostaining data of each section was randomly selected and measured by 2 different investigators in a blinded manner using the image analysis software (Image-Pro Plus, EPIX Vision).

Du X., Zhang S., Xiang Q., Chen J., Tian F., Xu J., Li X., Tan Y, & Liu L. (2020). The Role of a Selective P2Y6 Receptor Antagonist, MRS2578, on the Formation of Angiotensin II-Induced Abdominal Aortic Aneurysms. BioMed Research International, 2020, 1983940.

+ Open protocol

+ Expand

Quantifying Protein Expression Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GLUT1, FASN, and matrix metalloproteinase-2 (MMP2) expression levels were measured using Western blot analysis. Samples were lysed with a lysis buffer containing a protease inhibitor cocktail (G2006, Servicebio), and the protein concentrations were determined using a protein assay kit (G2006, Servicebio). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed with 20 μg of protein in each sample using 4% to 15% Mini Protean TGX precast gels (Servicebio). The following primary antibodies were used: anti-GLUT1 (AB652, 1:1000, Abcam), anti-FASN (AB22759, 1:1000, Abcam), and anti-MMP2 (GB11130, 1:1000, Servicebio). The bands were detected using a Western blotting detection system (Epson, Japan) and band intensity was calculated via densitometry using AlphaEaseFC (Alpha Innotech, USA).

Zhao J., Zhang Z., Nie D., Ma H., Yuan G., Su S., Liu S., Liu S, & Tang G. (2019). PET Imaging of Hepatocellular Carcinomas: 18F-Fluoropropionic Acid as a Complementary Radiotracer for 18F-Fluorodeoxyglucose. Molecular Imaging, 18, 1536012118821032.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Liver Fibrosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before immunostaining, paraffin-embedded sections were dewaxed and rehydrated in an alcohol series. The sections were washed with phosphate-buffered saline after heat-induced antigen retrieval. Next, the sections were treated with 3% hydrogen peroxide for 15 m to eliminate endogenous peroxidase activity and then incubated with 3% bovine serum albumin for 30 m at 37°C to block non-specific binding sites. Subsequently, sections were incubated overnight at 4°C with diluted primary antibodies, including α-SMA (1:2,000; GB13044; Servicebio, Inc. Wuhan, China), collagen I (1:1,200; GB11022-3; Servicebio, Inc.), matrix metalloproteinase (MMP)-2 (1:1,200; GB11130; Servicebio, Inc.), tissue inhibitors of metalloproteinase-1 (TIMP-1) (1:1,000; 106164-T40; Sino Biological US, Chesterbrook, PA, USA), TGF-β1 (1:500; GB11179; Servicebio, Inc.), and incubated with a conjugated secondary antibody after washing with phosphate-buffered saline (G0002; Servicebio, Inc.). Diaminobenzidine kits (G1211; Servicebio, Inc.) were used to visualize antibody binding areas. For each mouse, we analyzed three liver slices and randomly observed the positive-stained areas in three different fields of vision on each slice. The ratio of positive-stained to total area was calculated by Image-Pro Plus 6.0.

Liu F., Sun C., Chen Y., Du F., Yang Y, & Wu G. (2021). Indole-3-propionic Acid-aggravated CCl4-induced Liver Fibrosis via the TGF-β1/Smads Signaling Pathway. Journal of Clinical and Translational Hepatology, 9(6), 917-930.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Cervical Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

USL specimens were obtained from tissues extracted by hysterectomy. Samples (approximately 5 mm³ in size) were taken from the dorsal part of the cervix, 1 cm from the beginning of the cervix (at the left side), and verified by histological analysis.
Tissue samples were fixed in 10% neutral buffered formalin, embedded in paraffin, and treated with citric acid buffer (pH 6.0) in a microwave for antigen retrieval. Sections were then incubated in 3% hydrogen peroxide solution at room temperature in the dark for 25 min to inhibit endogenous peroxidases, then blocked in 3% bovine serum albumin at room temperature for 30 min. Reactions with primary antibodies against CD44 (dilution 1:100; GB13065), MMP-2 (dilution 1:500; GB11130), MMP-9 (dilution 1:500; GB11132-2), and TGF-β (dilution 1:100; GB13028) (all from Servicebio, Wuhan, China) were performed overnight at 4°C. After incubation with horseradish peroxidase-labeled secondary antibodies (Servicebio) at room temperature for 50 min, immune reactivity was detected with diaminobenzidine (K5007, DAKO, Wuhan, China); color development was observed under a microscope. Nuclei were counterstained with Harris hematoxylin. The percentages of positively stained areas were quantified using ImageJ software (version 1.8.0, National Institutes of Health, Bethesda, MD, USA).

Ying W., Hu Y, & Zhu H. (2022). Expression of CD44, Transforming Growth Factor-β, and Matrix Metalloproteinases in Women With Pelvic Organ Prolapse. Frontiers in Surgery, 9, 902871.

+ Open protocol

+ Expand

Comprehensive Immunohistochemical Profiling of Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Due to IHC and Wes-ProteinSimple, the following primary antibodies were used: primary rabbit polyclonal antibodies to Ki67 (GB111141, Servicebio), to CEACAM5 (DF6152, affinity), VEGFA (AF5131, affinity), VEGFC (DF7011, Affinity), Histone H3 (AF0863, affinity), ATP1A1 (AF6083, affinity), collagen type I (Proteintech, 14695-1-AP), MMP2 (GB11130, Servicebio), MMP9 (AF0220, Affinity), vimentin (AF7013, Affinity), TGF-β1 (AF7013, Affinity), E-cadherin (DF7157, Affinity), N-cadherin (AF5239, Affinity), rabbit monoclonal antibodies to TTF-1 (ab133737, abcam), to CD105 (ab221675, abcam) and mouse monoclonal antibodies to β-acting (sc47778, santa), to p63 (GB14129, Servicebio). Moreover, primary antibodies were used in combination with HRP-conjugated secondary antibodies (Jackson).

Ge Z., Wu S., Qi Z, & Ding S. (2022). Compared with High-intensity Interval Exercise, Moderate Intensity Constant Load Exercise is more effective in curbing the Growth and Metastasis of Lung Cancer. Journal of Cancer, 13(5), 1468-1479.

+ Open protocol

+ Expand

Protein Expression Analysis Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The assays were performed as described previously [37 (link)]. Antibody recognizing KDELR2 (1:1000, DF4047) was obtained from Affinity Biosciences, China. Antibodies for KIF20A (1:1000, A15377) and β-Actin (1:10,000, AC004) were obtained from ABclonal. Antibodies for MMP2 (1:1000, GB11130) and MMP9 (1:1000, GB11132) were obtained from Servicebio, Wuhan, China.

Meng X., Li W., Yuan H., Dong W., Xiao W, & Zhang X. (2022). KDELR2-KIF20A axis facilitates bladder cancer growth and metastasis by enhancing Golgi-mediated secretion. Biological Procedures Online, 24, 12.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Tissue Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed and paraffin-embedded tissues were cut into serial sections with a thickness of 4 µm. Sections were stained with hematoxylin and eosin (HE) for histological examination.
The paraffin sections were deparaffinized and rehydrated. Then, the sections were placed in citrate buffer (pH 6.0) for 10 min and washed with phosphate-buffered saline. After blocking with 3% BSA solution for 30min at room temperature, the sections were incubated with CHI3L antibody 1 (1:200, ab77528, Abcam), MMP-2 antibody (1:400, GB11130, Servicebio, Wuhan, China) and VEGF-A antibody (1:300, GB14165, Servicebio, Wuhan, China) overnight at 4 °C. The sections were washed with PBS and then incubated with the secondary antibodies for 1h at room temperature. The sections were then stained with 3, 3′-diaminobenzidine tetrahydrochloride for 10 min and counterstained with hematoxylin, dehydrated and mounted. Light yellow or brownish-yellow particles in the cytoplasm or

Zhang L., Wang Z., Li S., Liu X., Xu C, & Li L. (2023). The Potential Roles of CHI3L1 in Failed Autologous Arteriovenous Fistula in End-Stage Renal Disease. The Tohoku journal of experimental medicine, 259(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!