E1010

Manufactured by Applygen

Sourced in China

The E1010 is a compact laboratory centrifuge designed for general-purpose applications. It is capable of separating samples at high speeds to facilitate various experimental and analytical procedures.

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Lab products found in correlation

A019 2, by Nanjing Jiancheng (1 mentions) P1250, by Applygen (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Infinite 50, by Tecan (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Pcpt camp, by Merck Group (1 mentions) E1005, by Applygen (1 mentions) E1003, by Applygen (1 mentions) Recombinant human pdgf bb, by Thermo Fisher Scientific (1 mentions) A019 2 1, by Nanjing Jiancheng (1 mentions)

Spelling variants of this product

Glucose oxidase kits E1010 (2 citations)

11 protocols using e1010

Glycolysis Evaluation by Glucose and Lactate

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Glycolysis levels were evaluated by measuring glucose consumption and lactate production. The procedures were included as following. PGK1-overexpressing or PGK1-knockdown 786-O cells were seeded into 6-well plates at a same density of 1×10⁶ cells in 2.5 mL complete culture medium per well to culture for 24 h, then cell medium was collected to measure glucose consumption and lactate production level. At the same time, cells were collected to count to normalize the cell numbers between the experimental 786-O^-PGK1 (786-O^-shPGK1) and the control 786-O^-NC (786-O^-shNC) cells, which aimed to exclude the disturbance caused by cell growth difference on the relative level of glucose consumption and lactate production.
The concentrations of glucose consumption and lactate production were measured using a glucose assay kit (E1010, Applygen) and a lactate detection kit (A019, Nanjing Jiancheng Biotech) respectively, which were normalized by the cell numbers as described previously [26 (link), 27 (link)].

He Y., Wang X., Lu W., Zhang D., Huang L., Luo Y., Xiong L., Li H., Zhang P., Li Q, & Liang S. (2022). PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis. Cell Death & Disease, 13(2), 118.

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Glucose Starvation and Cell Metabolism

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Cells were seeded into 96‐well plates at a density of 3000/well. After being cultured for 24 h, cells were washed with PBS and incubated with a serum‐free medium overnight. Then, cells were washed with PBS and starved for glucose by pre‐incubating with 100 μL DMEM medium (without glucose) containing 2%BSA for 1 h. DMEM medium with 6 mM glucose containing 10% FBS was added to each well for 18 h. The supernatant was collected and performed MTT assay on cells in 96‐well plates for correction. The glucose concentration of the supernatant was quantified by a glucose consumption assay kit (E1010, Applygen Technologies, China) and data were determined using a microplate reader (MD, SpectraMax 190) at 550 nm.

Liu H., Lei W., Li Z., Wang X, & Zhou L. (2023). NR3C2 inhibits the proliferation of colorectal cancer via regulating glucose metabolism and phosphorylating AMPK. Journal of Cellular and Molecular Medicine, 27(8), 1069-1082.

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Vitamin K2 Modulates Glucose Metabolism

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Cells were seeded at a density of approximately 1×10⁶ cells per well, and treated with Vitamin K2 for the indicated time. The glucose consumption and lactate production were respectively assessed using the glucose and lactate content detecting kits (E1010, Applygen) (A019-2, Nanjing Jiancheng) according to the manufacturer’s protocols, and measured by microplate readers.

Duan F., Mei C., Yang L., Zheng J., Lu H., Xia Y., Hsu S., Liang H, & Hong L. (2020). Vitamin K2 promotes PI3K/AKT/HIF-1α-mediated glycolysis that leads to AMPK-dependent autophagic cell death in bladder cancer cells. Scientific Reports, 10, 7714.

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Glucose Quantification in PC12 Cells

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The concentration of glucose was detected using the glucose oxidase method (Li et al., 2015 (link)). The assay kit (E1010; Applygen Technologies, Beijing, China) was purchased from Applygen Technologies (Beijing, China). After treatment, the PC12 cells were collected, and the concentration of total protein was extracted using a total protein extraction kit (P1250; Applygen, Beijing, China) and detected using a BCA protein assay kit (P0012; Beyotime, Beijing, China). Then, according to the instructions, the absorbance was measured at 570 nm with an automatic microplate reader and calculated against a glucose standard curve.

Zhang Y., Huang N., Lu H., Huang J., Jin H., Shi J, & Jin F. (2020). Icariin protects against sodium azide—induced neurotoxicity by activating the PI3K/Akt/GSK-3β signaling pathway. PeerJ, 8, e8955.

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Glucose concentration measurement protocol

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The culture medium glucose concentration was measured by Glucose Oxidase Method with a commercial assay kit (Applygen Technologies, Cat# E1010). Samples were the −20 °C stocks of supernatant collected during each imaging experiment.

Liu Q., Sheng N., Zhang Z., He C., Zhao Y., Sun H., Chen J., Yang X, & Tang C. (2024). Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast. Heliyon, 10(5), e26558.

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Lactate and Glucose Metabolism Assay

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HSC3 and SCC15 cells were seeded in 6-well plates at 2.5 × 10⁵ cells per well and a well without cells as the control. After the cells were attached, they were switched to a complete medium. The medium was collected at 24 h of incubation to determine lactate production and glucose consumption. The respective concentrations of lactate production and glucose consumption were measured with commercial kits (a lactate assay kit [A019] from Nanjing Jiancheng Biotech, and a glucose assay kit [E1010] from Applygen, China) according to the manufacturer's instructions.

Cai H., Li J., Zhang Y., Liao Y., Zhu Y., Wang C, & Hou J. (2019). LDHA Promotes Oral Squamous Cell Carcinoma Progression Through Facilitating Glycolysis and Epithelial–Mesenchymal Transition. Frontiers in Oncology, 9, 1446.

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Insulin Resistance in FA-Induced Hepatic Cells

Cited 1 time

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The insulin resistance of high FA-induced hepatic cells was as described previously [27 (link)]. Hepa1-6 cells were seeded for 24 h. The cell culture medium was then exchanged with an FA-conditioned medium (25 mM glucose, and 0.5 mM palmitic acid and oleic acid) containing/not containing ginsenosides for 36 h. The cells were then treated with 1 nM insulin in serum-free low-glucose DMEM (5 mM glucose) for 12 h. The cell culture supernatants were collected for measurement (E1010, Applygen, Beijing, China) using a microplate reader (Infinite 50, Tecan, Mannedorf, Switzerland).

Yang S., Liu T., Hu C., Li W., Meng Y., Li H., Song C., He C., Zhou Y, & Fan Y. (2022). Ginsenoside Compound K Protects against Obesity through Pharmacological Targeting of Glucocorticoid Receptor to Activate Lipophagy and Lipid Metabolism. Pharmaceutics, 14(6), 1192.

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Gluconeogenesis Assay in Primary Hepatocytes

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After primary hepatocytes adhered to the plates, the culture medium was replaced with serum-free medium (M199 supplemented with 1% BSA and 1% PS) and cells were starved overnight. Glucose-producing solution, i.e., phenol red-free, glucose-free DMEM, containing substrates needed for gluconeogenesis (20 mmol/L lactate, 2 mmol/L pyruvate, 2 mmol/L L-glutamine, and 15 mmol/L HEPES) was prepared. Then, primary hepatocytes were incubated at 37°C for 6 h with a glucose-producing solution with, or without 0.1 mmol/L pCPT-cAMP (Sigma Aldrich, St. Louis, MO, United States) and 1 μmol/L dexamethasone (Sigma Aldrich). Two-hundred microliter of the culture medium were then collected, and the glucose concentration was measured using a colorimetric assay kit (glucose oxidase method; E1010; Applygen, Beijing, China).

Ying L., Zhang M., Ma X., Si Y., Li X., Su J., Yin J, & Bao Y. (2021). Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure. Frontiers in Cell and Developmental Biology, 9, 672032.

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Metabolic Profiling of DLK1-Deficient Mice

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The same batch of DLK1^−/− and wild-type mice were fasted for 12 h under free-drinking conditions. The next day, blood samples were collected from the posterior orbital venous plexus in 1.5 mL anticoagulant tubes containing potassium citrate and then centrifuged for 20 min at 3000 rpm at 4 °C. The supernatant was collected in a new 1.5 mL sterilized centrifuge tube for subsequent determination of plasma biochemical parameters. Plasma TG, CHOL, Glu, HDL and LDL levels were detected by Enzymic assay kit for total triglyceride, cholesterol, glucose, HDL, LDL in liquid samples (E1003, E1005, E1010, E1018 and E1017, Applygen Technologies, Beijing, China), which were used as described in the instructions. Then, the absorbance of the samples was detected using a multi-function microplate reader (Biotech, San Francisco, CA, USA).

Lu X., Fang X., Mi J., Liu Y., Liu R., Li G., Li Y, & Yang R. (2023). Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice. International Journal of Molecular Sciences, 25(1), 132.

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Quantifying Glucose and Lactate Levels

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The cells were cultivated in a six-well plate, and the cell culture supernatant transfected with si-PDGFRβ or stimulated with recombinant human PDGFBB (Peprotech, #101704, US) was collected at 24 h, 48 h, and 72 h. After that, the cell debris was removed by 1000 rpm × 10 min, and then according to the manufacturer's instructions, the follow-up studies were carried out using a glucose oxidase kit (E1010, Applygen Technologies, Beijing) or lactate assay kit (A019-2-1, NanJing Jian cheng, China). Finally, the glucose concentration was detected at 550 nm, and the lactate D r a f t
concentration was detected at 530 nm by a microplate reader.

Tang H.Y., Guo J.Q., Sang B.T., Cheng J.N, & Wu X.M. (2022). PDGFRβ modulates aerobic glycolysis in osteosarcoma HOS cells via the PI3K/AKT/mTOR/c-Myc pathway. Biochemistry and cell biology = Biochimie et biologie cellulaire, 100(1).

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