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Smarce1 baf57

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SMARCE1/BAF57 is a protein that is a component of the SWI/SNF chromatin remodeling complex. It plays a role in regulating gene expression by modifying chromatin structure. The core function of SMARCE1/BAF57 is to facilitate the remodeling of chromatin.

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Lab products found in correlation

Smarcb1 snf5, by Fortis Life Sciences (4 mentions) Arid1a, by Cell Signaling Technology (3 mentions) Arid1b, by Abcam (3 mentions) Smarca4 brg1, by Santa Cruz Biotechnology (3 mentions) Smarcc2 baf170, by Fortis Life Sciences (3 mentions) Smarcd1 baf60a, by Fortis Life Sciences (3 mentions) Actl6a baf53a, by Fortis Life Sciences (3 mentions) Actin, by Cell Signaling Technology (2 mentions) Smarcc1 baf155 antibody, by Santa Cruz Biotechnology (2 mentions) Arid1a antibody, by Merck Group (2 mentions) Arid1b antibody, by Santa Cruz Biotechnology (2 mentions) Protein g dynabead, by Thermo Fisher Scientific (2 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Actin hrp, by Santa Cruz Biotechnology (1 mentions) Eif4e, by BD (1 mentions) Prmt5, by Merck Group (1 mentions) Trip12, by Abcam (1 mentions) Cdc42, by BD (1 mentions)

4 protocols using smarce1 baf57

1

Immunoblotting of Chromatin Remodeling Proteins

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Immunoblotting was performed as previously described17 (link). Antibodies to the following proteins were used in the immunoprecipitation and immunoblots: SMARCC1/BAF155 (1:3000 dilution, Santa Cruz: sc9746); ARID1A (1:3000 dilution, Cell Signaling Technology: 12354 and home-made); ARID1B (1:1000 dilution, Abcam: ab54761); SMARCA4/BRG1 (1:500 dilution, Santa Cruz: sc17796 and home-made); SMARCC2/BAF170 (1:3000 dilution, Bethyl Laboratories: A301-039A); SMARCD1/BAF60A (1:3000 dilution, Bethyl Laboratories: A301-595A); SMARCE1/ BAF57 (1:3000 dilution, Bethyl Laboratories: A300-810A); SMARCB1/SNF5 (1:3000 dilution, Bethyl Laboratories: A301-087A); ACTL6A/BAF53A (1:3000 dilution, Bethyl Laboratories: A301-391A); ACTIN-HRP (1:3000 dilution, Santa Cruz: sc-47778).
Wolf B.K., Zhao Y., McCray A., Hawk W.H., Deary L.T., Sugiarto N.W., LaCroix I.S., Gerber S.A., Cheng C, & Wang X. (2022). Cooperation of chromatin remodeling SWI/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapes. Nature structural & molecular biology, 30(1), 10-21.
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2

Protein Expression Analysis in Prostate Cancer

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Procedures were previously described (Mounir et al., 2015 (link)) and the following antibodies were used at 1:1000 dilutions and incubated overnight at 4°C: ERG (Epitomics# 2805–1), PRMT5 (CST# 2252; SIGMA#P0493), GAPDH (Millipore#MAB374), H4 (CST#2592), AR (Santa Cruz#sc-7305), HA (Roche#11815016001), Symmetric Di-methyl arginine (SDMA, CST#13222), Mono-methyl arginine (MMA, CST#8711), TRIP12 (Abcam#ab86220), EIF4E (CST#9742), CDC42 (BD Transduction Laboratories#610929), HDAC1 (CST#2062), SMARCB1/SNF5 (Bethyl Laboratories# A301-087A), SMARCE1/BAF57 (Bethyl Laboratories# A300–810A).
Mounir Z., Korn J.M., Westerling T., Lin F., Kirby C.A., Schirle M., McAllister G., Hoffman G., Ramadan N., Hartung A., Feng Y., Kipp D.R., Quinn C., Fodor M., Baird J., Schoumacher M., Meyer R., Deeds J., Buchwalter G., Stams T., Keen N., Sellers W.R., Brown M, & Pagliarini R.A. (2016). ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. eLife, 5, e13964.
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3

Investigating Chromatin Remodeling Complexes

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Whole cell extracts of isogenic HCT116 cell lines were used in Western blots for E-Cadherin (Cell Signaling Technology: 24E10), ARID1A (Cell Signaling Technology: 12354), ARID1B (ABCAM ab57461) and ACTIN (Cell Signaling Technology: 5125). Nuclear extracts for co-immunoprecipitation were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Scientific #78835). Nuclear extracts were diluted with RIPA buffer (Life Technologies 89900) to a final concentration of 1 mg/ml (with protease inhibitor cocktails, Roche). Each IP was incubated with SMARCC1/BAF155 antibody (Santa Cruz: sc9746), ARID1A antibody (Millipore PSG3), or ARID1B antibody (Santa Cruz 32762) overnight at 4°C. Protein G Dynabeads (Life Technologies 10009D) were added and incubated at 4°C for 3 h. Beads were then washed three times with RIPA buffer and resuspended in reducing SDS gel loading buffer. Antibodies to the following proteins were used in the immunoblots: ARID1A (Cell Signaling Technology: 12354); ARID1B (Abcam: ab54761); SMARCA4/BRG1 (Santa Cruz: sc17796); BRM (Cell Signaling Technology: 11966); SMARCC2/BAF170 (Bethyl Laboratories: A301-039A); SMARCD1/BAF60A (Bethyl Laboratories: A301-595A); SMARCE1/ BAF57 (Bethyl Laboratories: A300-810A); SMARCB1/SNF5 (Bethyl Laboratories: A301-087A); ACTL6A/BAF53A (Bethyl Laboratories: A301-391A); ACTIN (Cell Signaling Technology: 5125, 1:3,000).
Mathur R., Alver B.H., San Roman A.K., Wilson B.G., Wang X., Agoston A.T., Park P.J., Shivdasani R.A, & Roberts C.W. (2016). ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice. Nature genetics, 49(2), 296-302.
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4

Investigating Chromatin Remodeling Complexes

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Whole cell extracts of isogenic HCT116 cell lines were used in Western blots for E-Cadherin (Cell Signaling Technology: 24E10), ARID1A (Cell Signaling Technology: 12354), ARID1B (ABCAM ab57461) and ACTIN (Cell Signaling Technology: 5125). Nuclear extracts for co-immunoprecipitation were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Scientific #78835). Nuclear extracts were diluted with RIPA buffer (Life Technologies 89900) to a final concentration of 1 mg/ml (with protease inhibitor cocktails, Roche). Each IP was incubated with SMARCC1/BAF155 antibody (Santa Cruz: sc9746), ARID1A antibody (Millipore PSG3), or ARID1B antibody (Santa Cruz 32762) overnight at 4°C. Protein G Dynabeads (Life Technologies 10009D) were added and incubated at 4°C for 3 h. Beads were then washed three times with RIPA buffer and resuspended in reducing SDS gel loading buffer. Antibodies to the following proteins were used in the immunoblots: ARID1A (Cell Signaling Technology: 12354); ARID1B (Abcam: ab54761); SMARCA4/BRG1 (Santa Cruz: sc17796); BRM (Cell Signaling Technology: 11966); SMARCC2/BAF170 (Bethyl Laboratories: A301-039A); SMARCD1/BAF60A (Bethyl Laboratories: A301-595A); SMARCE1/ BAF57 (Bethyl Laboratories: A300-810A); SMARCB1/SNF5 (Bethyl Laboratories: A301-087A); ACTL6A/BAF53A (Bethyl Laboratories: A301-391A); ACTIN (Cell Signaling Technology: 5125, 1:3,000).
Mathur R., Alver B.H., San Roman A.K., Wilson B.G., Wang X., Agoston A.T., Park P.J., Shivdasani R.A, & Roberts C.W. (2016). ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice. Nature genetics, 49(2), 296-302.
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