Hp1047a

Manufactured by Agilent Technologies

Sourced in United States

The HP1047A is a precision oscilloscope that provides high-quality waveform capture and analysis capabilities. It features a bandwidth of 100 MHz and can display signals with a sample rate of up to 1 GS/s. The HP1047A includes advanced triggering options and a range of measurement functions to support a variety of electronic testing and debugging applications.

Automatically generated - may contain errors

Lab products found in correlation

1260 infinity series system, by Agilent Technologies (2 mentions) Nad nadh quantitation kit, by Merck Group (1 mentions) Agilent 1100 series hplc system, by Agilent Technologies (1 mentions) Hp1100, by Agilent Technologies (1 mentions) 10 μm mixed b ls columns, by Wyatt Technology (1 mentions) Dawn eos malls detector, by Wyatt Technology (1 mentions) Nexera x2, by Shimadzu (1 mentions)

5 protocols using hp1047a

Monitoring Cell Growth and Metabolite Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell growth was monitored by measuring the OD₆₀₀ with an ultraviolet spectrophotometer (Beijing Puxi Universal Co. Ltd). The biomass concentration was calculated from OD₆₀₀ values using an experimentally determined correlation with 1 OD₆₀₀ unit being equal to 0.38 g/L cell dry weight (CDW) [42 (link)]. Glucose in the fermentation broth was determined utilizing a SBA sensor machine (Institute of Microbiology, Shangdong, China). To determine succinate, pyruvate, acetate and lactate concentrations, culture samples were centrifuged at 12,000g for 5 min and the aqueous supernatant used for HPLC analysis on an Agilent 1100 Series HPLC system equipped with a cation exchange column. (Aminex HPX87-H, Bio-Rad, Hercules, CA, USA), a UV absorbance detector (Agilent Technologies, G1315D) and a refractive index (RI) detector (Agilent Technologies, HP1047A). A mobile phase of 5 mM H₂SO₄ solution at a 0.4 mL/min flow rate was used. The column was operated at 65 °C. The intracellular NADH and NAD⁺ levels were determined by NAD/NADH Quantitation Kit (SIGMA) [47 (link)].

Meng J., Wang B., Liu D., Chen T., Wang Z, & Zhao X. (2016). High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli. Microbial Cell Factories, 15, 141.

+ Open protocol

+ Expand

Monitoring Bacterial Growth and Fermentation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bacterial growth was monitored by measuring the cell density at 600 nm (TU-1901, Persee, Beijing, China). Samples from the flask cultures were centrifuged at 13,000 rpm for 10 min, and the supernatant was stored at -20°C for future analysis. The sugar and fermentation products were determined using a high-performance liquid chromatograph (HP1100, Agilent Technologies, Palo Alto, USA) equipped with an ion exclusion Aminex HPX 87-H column (Bio-Rad, Richmond, USA), and 5 mM H₂SO₄ (0.4 ml/min) at 65°C was used as the mobile phase. The forms of D-xylose, L-arabinose and D-glucose were detected using a refractometer (Agilent, HP1047A), and acetoin was detected using an UV absorbance detector (Agilent, G1315D).

Zhang B., Li X.L., Fu J., Li N., Wang Z., Tang Y.J, & Chen T. (2016). Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis. PLoS ONE, 11(7), e0159298.

+ Open protocol

+ Expand

Characterization of Bottlebrush Polymers

Check if the same lab product or an alternative is used in the 5 most similar protocols

GPC was performed using an Agilent Technologies 1260 Infinity series system with two 5 μm mixed-D columns, a 5 μm guard column, a PL Gel 5 μm analytical Mixed-D column, and a RI detector (HP1047A); tetrahydrofuran (THF) was used as the eluent with a flow rate of 1.0 mL/min; polystyrene standards were used for the calibration.
MALS was performed in THF + 1 vol % triethylamine (TEA) using two Polymer Laboratories 10 μm mixed-B LS columns connected in series with a Wyatt Technologies DAWN EOS MALLS detector and RI detector at a flow rate of 1.0 mL/min. The dispersities (Đ) of each bottlebrush polymerization were measured by GPC, which was performed using dried THF as the eluent. MALS was used to determine the

M_{w}

of synthesized bottlebrushes, with an eluent of THF and 1% TEA and converted to

M_{n}

using the Đ obtained from THF GPC.

Clarke B.R, & Tew G.N. (2022). Bottlebrush Amphiphilic Polymer Co-Networks. Macromolecules, 55(12), 5131-5139.

+ Open protocol

+ Expand

Gel Permeation Chromatography Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

GPC was performed using an Agilent Technologies 1260 Infinity series system with two 5 μm mixed-D columns, a 5 μm guard column, a PL Gel 5 μm analytical Mixed-D column, and an RI detector (HP1047A); dried tetrahydrofuran (THF) was used as the eluent with a flow rate of 1.0 mL/min; polystyrene standards were used for the calibration. The dispersities (Ð) and molecular weights (M_w) of each macromonomer, cross-linker, and bottlebrush were measured using THF-GPC (Figures S1 and S4).

Nakada Y., Jiang Y., Nishijyo T., Itoh Y, & Lu C.D. (2001). Molecular Characterization and Regulation of the aguBA Operon, Responsible for Agmatine Utilization in Pseudomonas aeruginosa PAO1. Journal of Bacteriology, 183(22), 6517-6524.

+ Open protocol

+ Expand

Carbohydrate Analysis in Strawberry Fruits

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbohydrate extraction and determination were performed according to Roussos et al. [30] (link), using a Shimadzu Nexera X2 HPLC system equipped with an LC-30AD pump. The separation of the carbohydrates was achieved through a Hamilton HC-75 cation exchange column, calcium form (Ca 2+ ) (305 mm × 7.8 mm, 9 µm) (Hamilton, Bonaduz, Switzerland), equilibrated at 80 • C with water as mobile phase, running at a flow of 0.6 mL min -1 . Three soluble sugars were detected (HP1047A, refractive index detector, Agilent, Santa Clara, CA, USA) in the strawberry fruits, i.e., sucrose, glucose, and fructose. Total sugar concentration was estimated by summing the concentrations of the individual sugars detected by HPLC. Final concentrations were expressed as mg g -1 fresh weight.
The sweetness index (SI) of the fruit, an estimate of the total sweetness perception, was calculated based on the relative amount and sweetness properties of each carbohydrate [31] (link). Each carbohydrate contributes to sweetness perception as following: fructose is 2.3 and sucrose 1.35 times sweeter than glucose and hence the SI was calculated as 1.00 * (glucose concentration) + 1.35 * (sucrose concentration) + 2.3 * (fructose concentration) [31] (link).

Ntanos E., Kekelis P., Assimakopoulou A., Gasparatos D., Denaxa N., Tsafouros A., & Roussos P.A. (2021). Amelioration Effects against Salinity Stress in Strawberry by Bentonite–Zeolite Mixture, Glycine Betaine, and Bacillus amyloliquefaciens in Terms of Plant Growth, Nutrient Content, Soil Properties, Yield, and Fruit Quality Characteristics. Applied Sciences, 11(19), 8796-8796.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!