The gastric contents were obtained from the stomach and centrifuged at 3,000 g for 20 min. The supernatant was assessed for pH (Toledo 320, Mettler, Switzerland).
Toledo 320
Manufactured by Mettler Toledo
Sourced in Switzerland
The Toledo 320 is a precision weighing instrument designed for laboratory use. It features a high-resolution digital display and a durable construction to provide accurate and reliable measurements. The core function of the Toledo 320 is to weigh samples and materials with precision.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using toledo 320
1
Gastric Content pH Determination
2
Effect of pH on Staphylococcus aureus Growth
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Normal TSB medium was adjusted to have a pH of 7.4. TSB culture mediums with different pH values (8, 8.5, 9, and 9.5) were prepared by adding a 1 M NaOH solution dropwise to normal TSB while monitoring the mixture with a pH meter (Mettler Toledo320, Zurich, Switzerland). The Staphylococcus aureus (ATCC25923) bacteria were inoculated in TSB medium with different pH values (7.4, 8, 8.5, 9, and 9.5) in 48-well plates at a density of 1 × 106 CFUs/mL. Then, the culture plates were incubated at 37 °C for 360 and 480 minutes. At each time point, the incubated bacterial suspensions were blended, plated onto TSA, incubated for 24 hours, and counted.
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