Rabbit anti pan cadherin

Manufactured by Merck Group

Sourced in Macao

Rabbit anti-pan-Cadherin is a laboratory reagent used to detect the presence of cadherin proteins in biological samples. Cadherins are a family of calcium-dependent cell-cell adhesion molecules that play a crucial role in maintaining tissue integrity and cell-cell interactions. This antibody recognizes a common epitope shared among different cadherin subtypes, allowing for the detection of multiple cadherin proteins simultaneously.

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Lab products found in correlation

Gtx128354, by GeneTex (2 mentions) Sc 71557, by Santa Cruz Biotechnology (2 mentions) Sc 6556, by Santa Cruz Biotechnology (2 mentions) Gfp 1010, by Aves Labs (2 mentions) Lsm 710, by Zeiss (2 mentions) Goat anti hnf4a, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Mouse anti n cadherin clone 32, by BD (1 mentions) Mouse anti active β catenin clone 8e7, by Merck Group (1 mentions) Mouse anti lamin a c, by BD (1 mentions) Alexa fluor 546 rabbit anti mouse igg, by Thermo Fisher Scientific (1 mentions) Dapi dihydrochloride, by Thermo Fisher Scientific (1 mentions) Mouse anti α tubulin clone dm1a, by Merck Group (1 mentions) Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions) Anti e cadherin clone 36, by BD (1 mentions) Dapt n n 3 5 difluorophenacetyl l alanyl s phenyl glycine t butyl ester, by Merck Group (1 mentions) Ab96415, by Abcam (1 mentions) Ab76997, by Abcam (1 mentions) Ab181598, by Abcam (1 mentions)

3 protocols using rabbit anti pan cadherin

Whole-mount in situ hybridization and immunofluorescence

Cited 2 times

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Whole-mount in situ hybridization was performed as previously described using the sox9b probe (30 (link)). IF on whole-mount animals or cryosections was performed as previously described (58 (link)) (59 (link)). Antibodies used include rabbit anti-Prox1a (1:100, GTX128354; GeneTex), goat anti-Hnf4a (1:50, sc6556; Santa Cruz), mouse anti-Alcama (1:20, zn-8; DSHB), rabbit anti-pan-Cadherin (1:5,000; Sigma), mouse anti-Mdr1 (1:300, sc-71557; Santa Cruz), mouse anti-Anxa4 (1:100, ab71286, aka 2F11; Abcam) (8 (link)), chicken anti-GFP (1:300, GFP1010; Aves Labs), goat anti-mCherry (1:500, LS-C204207; LSBio), rabbit anti-Sox9b (1:100; gift from Mizuki Azuma at the University of Kansas), rabbit anti-Cytokeratin 19 (CK19) (1:100; ET1601-6; HUABIO), and rabbit anti-SOX9 (1:100; ET1611-56; HUABIO).

Zhao C., Matalonga J., Lancman J.J., Liu L., Xiao C., Kumar S., Gates K.P., He J., Graves A., Huisken J., Azuma M., Lu Z., Chen C., Ding B.S, & Dong P.D. (2022). Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling. Proceedings of the National Academy of Sciences of the United States of America, 119(50), e2201097119.

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Cadherin and Beta-Catenin Assay

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The following primary antibodies were used: rabbit anti-Pan-cadherin (C3678, Sigma-Aldrich, St. Louis, MO) that recognizes the conserved C-terminal domain of classic cadherins, mouse anti-N-cadherin (clone 32) and anti-E-cadherin (clone 36), both from BD Biosciences (Franklin Lakes, New Jersey, USA), rabbit anti-β-catenin (Invitrogen-Molecular Probes, Carlsbad, CA), mouse anti-active-β-catenin (clone 8E7, Millipore, Billerica, MA, USA), mouse anti-lamin A∖C (BD Biosciences), and mouse anti-α-tubulin (clone DM1a, Sigma-Aldrich). Secondary antibodies were Alexa Fluor™ 488 goat anti-rabbit IgG, Alexa Fluor™ 546 rabbit anti-mouse IgG (Invitrogen, Life Technologies, Brazil, São Paulo, SP, Brazil), and peroxidase-conjugated goat anti-rabbit and rabbit anti-mouse (Promega, Madison, WI). DAPI dihydrochloride (Invitrogen) was used for nuclear staining. The γ-secretase activity inhibitor Dapt (N-N[-(3,5-Difluorophenacetyl-l-alanyl)]-S-phenylglycine-t-butyl-ester) was from Merck Biosciences (Darmstadt, Germany). Nuclear and cytoplasmic fractions were extracted using NE-PER® Nuclear and Cytoplasmic Extraction Reagents (Pierce Biotechnology, Rockford, IL).

Bonfim D.C., Dias R.B., Fortuna-Costa A., Chicaybam L., Lopes D.V., Dutra H.S., Borojevic R., Bonamino M., Mermelstein C, & Rossi M.I. (2016). PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells. Stem Cells International, 2016, 3865315.

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Multimarker Fluorescent Imaging of Liver

Cited 1 time

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Whole-mount fluorescent in situ hybridization was performed as previously described using the fgf10 probe(16 (link)). Whole mount IF staining was performed as previously described (16 (link)). Antibodies used include rabbit anti-Prox1a (1:100, GTX128354, GeneTex), goat anti-Hnf4a (1:50, sc6556, Santa Cruz), mouse anti-Alcama (1:20, zn-8, DSHB) and mouse anti-Islet1/2 (1:20, 39.4D5, DSHB), rabbit anti-pan-Cadherin (1:5000; Sigma), mouse anti-Anxa4 (1:100, ab71286, aka 2F11, Abcam)(15 (link)), rabbit anti-Bhmt (1:200, ab96415, Abcam), mouse anti-Mdr1(1:200, sc-71557, Santa Cruz), chicken anti-GFP (1:300, GFP1010, Aves Labs), goat anti-mCherry (1:500, LS-C204207, LSBio), rabbit anti-Cytokeratin7 (1:200, ab181598, Abcam), mouse anti-Sox9 (1:200, ab76997, Abcam). Imaging was performed on a Zeiss LSM710 running Zen 2010 (Black) and images were processed with Adobe Photoshop.

Zhao C., Lancman J.J., Yang Y., Gates K.P., Cao D., Barske L., Matalonga J., Pan X., He J., Graves A., Huisken J., Chen C, & Dong P.D. (2021). Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome. Hepatology (Baltimore, Md.), 75(3), 567-583.

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