Rabbit polyclonal anti gapdh 2118

Manufactured by Cell Signaling Technology

Rabbit polyclonal anti-GAPDH (2118) is a laboratory reagent used to detect the presence of the GAPDH protein in biological samples. It is a primary antibody produced in rabbits that specifically binds to the GAPDH protein.

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Lab products found in correlation

Mouse monoclonal anti lamp1 h4a3, by Santa Cruz Biotechnology (2 mentions) Peroxidase conjugated secondary antibodies for western blotting, by Merck Group (1 mentions) Dpy30, by Fortis Life Sciences (1 mentions) Rbbp5, by Fortis Life Sciences (1 mentions) Ash2l, by Fortis Life Sciences (1 mentions) Anti gfp 6556, by Abcam (1 mentions)

2 protocols using rabbit polyclonal anti gapdh 2118

Antibodies for Protein Analysis

Cited 2 times

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Rabbit polyclonal DPY30, RBBP5, ASH2L and BIG1 antibodies were from Bethyl Laboratories. Rabbit polyclonal anti-GFP (6556) and mouse monoclonal anti-M6PR (ab2733) were from Abcam. Mouse monoclonal anti-Snx1 was obtained from BD Transduction Laboratories. Mouse monoclonal anti-LC3 was obtained from MBL (clone 4E12, catalogue number M152-3). Rabbit polyclonal anti-spastin 86-340 and CD8 antibodies were produced as previously described (Seaman, 2004 (link); Connell et al., 2009 (link)). Mouse monoclonal anti-LAMP1 (H4A3) was obtained from Santa Cruz Biotechnology. Rabbit polyclonal anti-GAPDH (2118) was from Cell Signalling Technology. Alexa Fluor® 488 and 568 labelled secondary antibodies for immunofluorescence were obtained from Molecular Probes. Peroxidase conjugated secondary antibodies for western blotting were obtained from Sigma.

Newton T., Allison R., Edgar J.R., Lumb J.H., Rodger C.E., Manna P.T., Rizo T., Kohl Z., Nygren A.O., Arning L., Schüle R., Depienne C., Goldberg L., Frahm C., Stevanin G., Durr A., Schöls L., Winner B., Beetz C, & Reid E. (2018). Mechanistic basis of an epistatic interaction reducing age at onset in hereditary spastic paraplegia. Brain, 141(5), 1286-1299.

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Spastin Antibody Protocol

Cited 1 time

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Rabbit polyclonal anti-spastin (86–340) (raised against a glutathione S-transferase fusion protein that incorporated residues 86–340 of M1-spastin) antibodies were produced as previously described (Connell et al., 2009 (link)). Mouse monoclonal α-tubulin (DM1A) and peroxidase-conjugated secondary antibodies for Western blotting were obtained from Sigma-Aldrich; mouse monoclonal anti-myc (4A6) from EMD Millipore; mouse monoclonal anti-SNX1 (611482) from BD transduction laboratories; rabbit polyclonal anti-GFP (ab6556) and mouse monoclonal anti-M6PR (ab2733) from Abcam; mouse monoclonal anti-LAMP1 (H4A3) from Santa Cruz Biotechnology, Inc.; rabbit polyclonal anti-GAPDH (2118) from Cell Signaling Technology; and Alexa Fluor 488– and 568–labeled secondary antibodies for immunofluorescence from Molecular Probes.

Allison R., Edgar J.R, & Reid E. (2019). Spastin MIT Domain Disease-Associated Mutations Disrupt Lysosomal Function. Frontiers in Neuroscience, 13, 1179.

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