Heracell vios 160i cr co2 incubator

Five PDAC cell lines (PANC-1, MIA-Paca 2, SW1990, BxPC-3 and CFPAC) were kept at our laboratory. The cells were maintained in an incubator (Heracell™ Vios 160i CR CO₂ Incubator, Thermo Fisher Scientific, Waltham, MA, USA) with a standard pathogen-free environment (humidified, 37 °C, 5% CO₂). PANC-1 cells, MIA-Paca 2 cells and SW1990 cells were cultured in Dulbecco’s minimal essential medium (DMEM). BxPC-3 cells and CFPAC cells were cultured in Rosewell Park Memorial Institute-1640 medium (RPMI-1640). All of the cell culture media contained 10% fetal calf serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Furthermore, antibiotics (penicillin and streptomycin) were also added into the media under the proper conditions (100 U/mL for penicillin; 100 µg/mL for streptomycin). When the degree of cell fusion reached approximately 90%, the cells were dissociated and harvested with the help of trypsin. Then, the cells were passaged properly using standard techniques.

Breast cancer cell lines (SK-BR-3 (HTB-30) and MCF7 (HTB-22)) and culture media were obtained from American Type Culture Collection (ATCC, USA). SK-BR-3 cells were cultivated in McCoy’s 5a Medium Modified (30–2007) supplemented with 10% fetal bovine serum (FBS, 164,210–500, Procell). MCF7 cells were cultured in Eagle’s Minimum Essential Medium (30–2003) added with 0.01 mg/ml human recombinant insulin (91077C, Sigma-Aldrich, USA) and 10% FBS. The incubation was carried out at 37°C with 5% CO₂ (Heracell Vios 160i CR CO2 incubator, 51,033,770, Thermo Scientific, USA).