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Heracell vios 160i cr co2 incubator

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Heracell™ Vios 160i CR CO2 incubator is a laboratory equipment designed for cell culture applications. It provides a controlled environment for the incubation of cell lines, tissues, or other biological samples. The incubator maintains precise temperature, humidity, and CO2 levels to support the optimal growth and proliferation of cell cultures.

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3 protocols using heracell vios 160i cr co2 incubator

1

Ovarian Cancer Organoid Establishment

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Fresh obtained clinical tissues were washed three times with an organoid washing buffer (LSTO00100201; Shanghai LiSheng Biotech, China) to rinse the mucus and debris. Then, the tissues were mechanically sheared using and Ovarian Cancer Tissue Sampling Kit (LSTO00100101, Shanghai LiSheng Biotech, China). Next, 3- to 6-mm tissue debris was seeded in 6-well culture plates (#3516, Costar) and cultured with 6-mL OC organoid medium (LSTO001004, Shanghai LiSheng Biotech, China) in a Heracell™ Vios 160i CR CO2 incubator (51033770, ThermoFisher) at 37℃ under a humidified atmosphere with 5% CO2. Approximately 50% medium was changed every 5 days, to provide sufficient nutrients for the organoids. After the tumor pieces developed into organoids, they were passaged every 15 days according to the manufacturer’s instructions. If the pieces were circular and presented the characteristics of the parental tumor within 7 days, the culture was regarded as successful.
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2

Culturing Pancreatic Cancer Cell Lines

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Five PDAC cell lines (PANC-1, MIA-Paca 2, SW1990, BxPC-3 and CFPAC) were kept at our laboratory. The cells were maintained in an incubator (Heracell™ Vios 160i CR CO2 Incubator, Thermo Fisher Scientific, Waltham, MA, USA) with a standard pathogen-free environment (humidified, 37 °C, 5% CO2). PANC-1 cells, MIA-Paca 2 cells and SW1990 cells were cultured in Dulbecco’s minimal essential medium (DMEM). BxPC-3 cells and CFPAC cells were cultured in Rosewell Park Memorial Institute-1640 medium (RPMI-1640). All of the cell culture media contained 10% fetal calf serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Furthermore, antibiotics (penicillin and streptomycin) were also added into the media under the proper conditions (100 U/mL for penicillin; 100 µg/mL for streptomycin). When the degree of cell fusion reached approximately 90%, the cells were dissociated and harvested with the help of trypsin. Then, the cells were passaged properly using standard techniques.
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3

Breast Cancer Cell Line Culture

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Breast cancer cell lines (SK-BR-3 (HTB-30) and MCF7 (HTB-22)) and culture media were obtained from American Type Culture Collection (ATCC, USA). SK-BR-3 cells were cultivated in McCoy’s 5a Medium Modified (30–2007) supplemented with 10% fetal bovine serum (FBS, 164,210–500, Procell). MCF7 cells were cultured in Eagle’s Minimum Essential Medium (30–2003) added with 0.01 mg/ml human recombinant insulin (91077C, Sigma-Aldrich, USA) and 10% FBS. The incubation was carried out at 37°C with 5% CO2 (Heracell Vios 160i CR CO2 incubator, 51,033,770, Thermo Scientific, USA).
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