Ez slide

Manufactured by Merck Group

Sourced in United States

EZ slides are a type of microscope slide designed for easy handling and viewing of samples. The slides feature a smooth, non-reflective surface and are made from high-quality materials to ensure durability and clarity. EZ slides are suitable for a variety of applications in research, diagnostics, and education.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (4 mentions) Axio observer z1, by Zeiss (3 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions) Goat anti rabbit secondary antibody, by Abcam (1 mentions) Alexa fluor 647, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Fluorescence microscope, by Olympus (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) H2dcfda, by Merck Group (1 mentions) Axio imager m1, by Zeiss (1 mentions) Infinite m1000, by Tecan (1 mentions) Axio observer z1 fluorescent microscope, by Zeiss (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Imaris, by Oxford Instruments (1 mentions) Axio imager m2, by Zeiss (1 mentions) Vectashield mounting medium with dapi h 1200, by Vector Laboratories (1 mentions) Dnmt1 antibody, by Abcam (1 mentions) Dnmt3a antibody, by Abcam (1 mentions)

Close spelling variants of this product

Millicell four-well glass EZ slides EZ chamber slides Millicell EZ 8-well chamber slides Millicell EZ 8-well slides EMD Millipore Millicell EZ Slides Millicell EZ SLIDES eight-well glass slides Millicell EZ 24‐well glass slides Millicell EZ chamber slides Millicel®EZ slides EZ-multiwell slides

18 protocols using ez slide

Immunofluorescence of Cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary cardiomyocytes were fixed with 4% paraformaldehyde (v/v) in EZ slides (Millipore, Billerica, MA) for 15 minutes, permeabilized with 0.3% Triton X-100 (v/v) for 15 minutes, and blocked with 5% bovine serum albumin for 30 minutes at room temperature. The cells were incubated with mouse anticardiac troponin T (cTnT) primary antibodies (1:100; cat. No. MA5-12960; Invitrogen) overnight at 4°C, followed by Alexa Fluor 488-conjugated secondary antibodies (1:100; cat. No. A32723; Invitrogen) at 37°C for 45 minutes. Finally, the cells were stained with DAPI (Solarbio) at room temperature for 5 minutes. Images were captured using an Olympus AX70 laser confocal microscope at excitation wavelengths of 495 nm or 358 nm and detected at 518 nm or 461 nm.

Wang Y., Li X., Xu X., Qu X, & Yang Y. (2022). Transient Receptor Potential Vanilloid Type 1 Protects Against Pressure Overload–Induced Cardiac Hypertrophy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes. Journal of Cardiovascular Pharmacology, 80(3), 430-441.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The adherent cells were trypsinized and plated on EZ slides (Millipore, Billerica, MA, USA) for immunofluorescence assays. The cells were labeled with monoclonal rat anti-CD90 antibody (Abcam, Cambridge, MA, USA), followed by donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (Thermo Scientific, Waltham, MA, USA). For c-Kit staining, cells were incubated with polyclonal rabbit anti-c-Kit (Abcam) antibody, followed by incubation with goat anti-rabbit secondary antibody conjugated with Alexa Fluor 647 (Abcam). After incubation with 4′,6-diamidino-2-phenylindole (Thermo Scientific) for 1 min, the cells were observed under a fluorescence microscope (Olympus, Tokyo, Japan). The percentages of c-Kit⁺ and CD90⁺ cells during each isolation were analyzed using Image J software.

Li W., Xu H, & Qian C. (2017). c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer. BioMed Research International, 2017, 7407168.

+ Open protocol

+ Expand

Quantifying Intracellular ROS Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell permeant reagent 2’,7’ – dichlorodihydrofluorescein diacetate (H₂DCFDA), a fluorogenic dye (Life Technologies) was utilized to study ROS generation in H9c2 and neonatal cardiac cells. Briefly, cells were plated onto a 96 black well plate and allowed to reach a confluency of ~80%, washed twice with PBS, and then treated with 10µM of H₂DCFDA in phenol red free media for 30 min at 37°C (n=4–9). Following incubation, the wells were again washed with PBS and the cells were treated with different concentrations of DIC and H₂O₂ in 5% FBS in phenol free media. ROS generation was determined immediately by measuring the formation of fluorescent DCF, using an Infinite M1000 PRO-Tecan, at an Ex-490nm and Em-520nm. Measurements were done every 10 minutes for 3 hours.
Fluorescence microscopy was also employed to detect the intensity of fluorescence in these cells in the presence of DIC and rotenone. Rotenone was used at a lower concentration (20 µM) than what was previously found to increase ROS production in another cell type [33 (link)]. Briefly, cells were plated in 8 well slides (EZ slides, Millipore) and incubated with H₂DCFDA for 30 min. Thereafter, the cells were treated with the compounds for 1.5 h and the fluorescence intensity was measured immediately with a Zeiss Axio Imager M1. Three or more independent experiments were performed for each group.

Ghosh R., Goswami S.K., Feitoza L.F., Hammock B, & Gomes A.V. (2016). Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts. International journal of cardiology, 223, 923-935.

+ Open protocol

+ Expand

Immunostaining Assay for IL-13 Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

These assays were performed as described previously [9 (link)].Twenty thousand cells were plated in 8 well glass chambered EZ slides (Millipore, Billerica, MA, USA) overnight at 37 °C in a CO₂ incubator and treated with IL-13 at 20 ng/ml for 30 min, 120 min, 240 min and overnight. IL-2 was used as a negative control. Antibodies and staining protocol was similar to cells and tissue sections as indicated in the staining protocols. The extent of immunostaining was ascertained by evaluating the staining intensity and counting number of positive cells in a set of 200 total cells or number of positive fields in biopsy specimens to calculate % positive cells or fields after viewing the samples at 200X magnification.

Bhardwaj R., Suzuki A., Leland P., Joshi B.H, & Puri R.K. (2018). Identification of a novel role of IL-13Rα2 in human Glioblastoma multiforme: interleukin-13 mediates signal transduction through AP-1 pathway. Journal of Translational Medicine, 16, 369.

+ Open protocol

+ Expand

Visualizing G3BP1 Stress Granule Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

WT eIF2α and mutant eIF2α S51A MEFs were seeded onto 8-well chamber slides (Millipore, EZ Slides) and infected with VVΔE3L at MOI = 1. At 8 hpi, cells were fixed with 4% Formaldehyde for 1 hr at room temperature (RT), followed by two washes with PBS, and permeabilization with cold methanol for 5 min. Cells were rinsed twice in PBS before incubation with Blocking buffer (5 mg/mL BSA and 0.04% Tween 20 in PBS) for 30 min at RT. Cells were overnight at 4°C incubated in primary anti-G3BP1 (BD Biosciences, #611126), prepared in Blocking buffer at 3 ug/mL. After three washes in PBS, cells were incubated in anti-mouse secondary (Life Sciences, AlexaFluor 588) at 1:200 in Blocking buffer for 1hr at RT. This was followed by three additional washes in PBS and incubation with DAPI at 1:1000 in PBS for 1min. Finally, cells were washed five times in PBS before mounting (Vectashield). G3BP1 and DAPI expression was visualized using the Nikon DS-Qi1 inverted fluorescent microscope.

Pham A.M., Santa Maria F.G., Lahiri T., Friedman E., Marié I.J, & Levy D.E. (2016). PKR Transduces MDA5-Dependent Signals for Type I IFN Induction. PLoS Pathogens, 12(3), e1005489.

+ Open protocol

+ Expand

Autophagy Monitoring by GFP-LC3 Puncta

Check if the same lab product or an alternative is used in the 5 most similar protocols

Autophagy levels were evaluated by monitoring cellular localization of GFP-tagged LC3 (36 (link)). 2×10⁵ COS7 or MCF7 cells were seeded in each chamber of four-well culture slides (Millipore EZ slides) and cultured overnight in DMEM with 10% FBS until ~80% confluency. Lipofectamine 2000 was used for transfection as above, with cells in each chamber being co-transfected with 4 μg of total plasmids comprising 1.6 μg GFP-LC3; and either 1.2 μg WT BECN1 and 1.2 μg WT or mutant ATG14 expression plasmids for COS7 cells; or 1.6 μg GFP-LC3 and 1.2 μg WT ATG14 and 1.2 μg WT or mutant BECN1 expression plasmids for MCF7 cells. After transfection, the cells were cultured in either rich media (DMEM, 10% FBS, 2X amino acid mixture, GIBCO) or starvation media (Earle’s balanced salt solution, GIBCO) for 4 hours. Then the cells were fixed with 4% paraformaldehyde in PBS. GFP-LC3-positive puncta were observed using a Zeiss AxioObserver Z1 fluorescent microscope and quantified by counting a minimum of 50 cells per condition in three independent repeats using the Imaris program (Bitplane). The significance of alterations in autophagy levels was determined by a two-tailed, heteroscedastic student’s t-test, wherein p ≤ 0.05 is considered significant.

Mei Y., Su M., Sanishvili R., Chakravarthy S., Colbert C.L, & Sinha S.C. (2016). Identification of BECN1 and ATG14 coiled-coil interface residues important for starvation-induced autophagy. Biochemistry, 55(30), 4239-4253.

+ Open protocol

+ Expand

Quantifying Autophagy in MCF7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular autophagy levels were evaluated by monitoring cellular localization of GFP-tagged LC3 (63 (link)). 5.0 × 10⁵ MCF7 cells were seeded in each chamber of a 4-well culture slide (Millipore EZ slides) and cultured overnight in DMEM with 10% FBS until ~80% confluency. The cells were co-transfected with a total of 4 µg plasmids comprising 1.6 µg GFP-LC3 and 2.4 µg BECN1 WT or mutant expression plasmids, using lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. After transfection, the cells were cultured in either rich (DMEM, 10% FBS, 2X amino acid mixture) or starvation (EBSS) media respectively for 4 hours. Cells were then fixed with 2% paraformaldehyde in PBS. GFP-LC3-positive puncta were observed under a fluorescent microscope (Zeiss AxioObserver Z1) and quantified by counting a minimum of 100 cells for duplicate samples per condition using Imaris (Bitplane AG, Zurich, Switzerland) in three independent repeats. The significance of alterations in autophagy levels was determined by a two-tailed, heteroscedastic student’s t-test, wherein p ≤ 0.05 is considered significant.

Mei Y., Ramanathan A., Glover K., Stanley C., Sanishvili R., Chakravarthy S., Yang Z., Colbert C.L, & Sinha S. (2016). Conformational Flexibility Enables Function of a BECN1 Region Essential for Starvation-Mediated Autophagy. Biochemistry, 55(13), 1945-1958.

+ Open protocol

+ Expand

Visualizing Mitochondrial DNA in ARPE-19 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

ARPE-19 cells were seeded on EZ Slides (PEZGS0416, Millipore) at 5x10⁴ cells/well in antibiotic-free medium and allowed to settle overnight. The following day, cells were transfected with 1µg/mL of FAM-tagged mtDNA using HP X-tremeGene agent at a ratio of 1:1 according to the manufacturer’s protocol. 24 hours later, cells were carefully washed with PBS before fixation in 4% PFA for 5 minutes. Afterwards, cells were washed again with PBS and mounted using Vectashield mounting medium with DAPI (H-1200) (Vector Laboratories, Burlingame, CA). Slides were visualized on Zeiss Axio Imager M2.

Dib B., Lin H., Maidana D.E., Tian B., Miller J.B., Bouzika P., Miller J.W, & Vavvas D.G. (2015). Mitochondrial DNA has a pro-inflammatory role in AMD. Biochimica et biophysica acta, 1853(11 Pt A), 2897-2906.

+ Open protocol

+ Expand

RPTECs-based in vitro DN Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPTECs were cultured on the EZ slides (Merck Millipore). Treatment of Ang-II, AGE and HG was given to the cells and subsequent transfection with miR29b mimics and miR29b inhibitors was carried out. The cells were fixed using 4% paraformaldehyde. RPTECs based in vitro DN model was incubated with primary DNMT3A antibody (1:1000, Abcam), DNMT3B antibody (4 μg/ml, Sigma), DNMT1 antibody (1 μg/ml, Abcam) for overnight at 4 ºC. After overnight incubation with primary antibody, the slides were washed with PBS (3 times). The slides were then incubated with secondary antibody goat anti-rabbit IgG-FITC for 2 h. After complete incubation, slides were again washed with PBS (3 times) and counter stained with DAPI for cell nuclei. Slides were then observed under the microscope and images were quantified using Image J software.

Gondaliya P., Dasare A., Srivastava A, & Kalia K. (2018). miR29b regulates aberrant methylation in In-Vitro diabetic nephropathy model of renal proximal tubular cells. PLoS ONE, 13(11), e0208044.

+ Open protocol

+ Expand

Fluorescence Imaging of SNAP-EAP45 Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa or HeLa-TSG101-GFP cells were seeded into 8-well slides (EZ slides, Merck). At 24-hour post seeding, the cells were transfected with 100 ng of either SNAP-FL, SNAP-∆G, or SNAP-∆G∆H-EAP45 expressor. This was followed by fixation with paraformaldehyde and permeabilisation with Triton X-100 and staining with SNAP-Surface AlexaFluor647 (NEB) at 4 μM concentration for SNAP-EAP45 according to the manufacturer's instructions and DAPI for nuclei on the following day. A primary antibody to RAB7 (Abcam) or anti-Tubulin (Sigma) was also added on some occasions followed by staining with the secondary antibody conjugated with either AlexaFluor488 or AlexaFluor647 (Thermofisher) before imaging using Leica SP5 confocal fluorescence microscope. The fluorescence intensity was calculated within the dark zone during cytokinesis for the SNAP channel. The normalised fluorescence intensity was obtained by subtracting the fluorescence intensity from the averaged non-transfected (NT) cells for each experiment. Negative values were obtained for those where the fluorescence intensities are smaller than the averaged background from NT. To compensate these, a constant value was used to transform all the data points before statistical analyses were carried out.

Meng B., Vallejo Ramirez P.P., Scherer K.M., Bruggeman E., Kenyon J.C., Kaminski C.F, & Lever A.M. (2021). EAP45 association with budding HIV-1: Kinetics and domain requirements. Traffic (Copenhagen, Denmark), 22(12).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!