Immunohistochemical staining was performed on routine sections. The following monoclonal antibodies were used for immunostaining: AE1/AE3 (clone AE1/3, 1:50 dilution; Dako, Carpinteria, CA), CK7 (clone OV-TL 12/30, prediluted; Dako), CK5/6 (D5/16B4 clone, prediluted; Dako), CK20 (clone Ks20.8, prediluted; Dako), synaptophysin (clone DAK-SYNAP, prediluted; Dako), chromogranin (clone LK2H10+PHE5, 1:200 dilution; Thermo Scientific, Waltham, MA), CD56 (clone 123C3, 1:50 dilution; Dako), TTF1 (clone SPT24, prediluted; Leica Biosystems, Buffalo Grove, IL), INSM1 (clone A-8, 1:150; Santa Cruz Biotechnology, Santa Cruz, CA), GATA3 (clone L50-823, 1:100 dilution; Cell Marque, Rocklin, CA), p63 (clone DAK-p63, prediluted; Dako), p40 (clone BC-28, 1:50 dilution; Biocare, Concord, CA), and uroplakin II (clone BC-21, 1:100 dilution; Biocare). Briefly, 4-μm-thick sections were deparaffinized in xylene and hydrated in graded alcohol. Immunostaining was performed using a DAKO autostainer (Agilent, Santa Clara, CA). Slides were incubated with the primary antibody and then with a visualization reagent (secondary goat anti-mouse immunoglobulin and horseradish peroxidase linked to a dextran polymer backbone). The slides were then rinsed with distilled water, incubated with a 3,3-diaminobenzidine substrate-chromogen solution, and subjected to Mayer hematoxylin counterstaining.
P40 clone bc 28
Manufactured by Biocare Medical
Sourced in United Kingdom
The P40 (clone BC-28) is a monoclonal antibody used in immunohistochemistry applications. It targets the p40 protein, which is a p63 isoform. The antibody can be used to detect the presence of the p40 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Dako autostainer, by Agilent Technologies (1 mentions)
Ck7 clone ov tl 12 30, by Agilent Technologies (1 mentions)
Ck20 clone ks20, by Agilent Technologies (1 mentions)
Cd56 clone 123c3, by Agilent Technologies (1 mentions)
Insm1 clone a 8, by Santa Cruz Biotechnology (1 mentions)
Gata3 clone l50 823, by Cell Marque (1 mentions)
Bond max system, by Leica (1 mentions)
Ttf 1 clone 8g7g3 1, by Agilent Technologies (1 mentions)
Ck5 6 clone d5 16 b4, by Agilent Technologies (1 mentions)
3 protocols using p40 clone bc 28
1
Immunohistochemical Profiling of Tumor Samples
2
Immunohistochemical Profiling of Lung Tumors
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The tissue sections were immunostained with commercially available antibodies including thyroid transcription factor-1 (TTF-1) (clone 8G7G3/1, Dako), p40 (clone BC28, Biocare Medical) and CK5/6 (clone D5/16 B4, Dako) on a Leica BOND-MAX system (Leica Microsystems, Newcastle, UK). Tumors completely absent of TTF-1 staining were considered as TTF-1 negative. For p40 or CK5/6 staining, the moderate staining pattern was defined as 10 to 50% of tumor cells showing immunoreactivity, while the diffuse staining pattern was defined as more than 50% of tumor cells with immunoreactivity. Cases with staining intensity similar to internal controls represented by bronchial/bronchiolar basal cell layer were recognized as strong intensity.
3
Mucin and IHC Characterization of NSCLC
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Consecutive 4 micrometer thick TMA sections were stained with PASD, ABPAS (pH 2.5 for alcian blue) and mucicarmine at the Department of Genetics and Pathology, Lund, Sweden. For mucicarmine, small intestine was used as control tissue. For PASD and ABPAS, small intestine and liver was used as control tissue but only for each run, not on each slide. IHC stained sections for p40, p63, CK5, TTF-1 and napsin A were available from previous investigations (these were not consecutive to the sections for mucin stains, but there was no significant difference in morphological appearance of the tumors between the sections)6 (link). The antibody clones and concentrations were p40 clone BC28 1:50 (Biocare Medical, Concord, CA), p63 clone 4A4 ready-to-use, TTF-1 clone 8G7G3/1 ready-to-use (both Ventana Medical Systems, Tucson, AZ), CK5 clone XM26 1:25, TTF-1 clone SPT24 1:100, and napsin A clone IP64 1:20 (all three Leica Biosystems, Nussloch, Germany). Control tissue was used on each slide. As part of the present study, IHC staining for CK7 was also performed with clone SP52 ready-to-use (Ventana) with liver as control tissue.
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