One step rt pcr kit

The total RNA was extracted from cells using an RNA Extraction Kit (Accurate Biotechnology, Danyang, China). Total RNA was reverse-transcribed to cDNA using a one-step RT-PCR kit (Accurate Biotechnology, China). RT-qPCR was performed using SYBR Green (Accurate Biotechnology, China) in an RT-PCR machine (Agilent Technologies, Santa Clara, CA, USA). Values were normalized to GAPDH mRNA levels. The gene primer sequences used for qRT-PCR are listed in Table 1.

Total RNA was isolated from liver samples using One-step RT-PCR Kit (Accurate Biology, Hunan, China), according to the manufacturer’s protocol. Total RNA concentration was determined using NanoDrop One spectrophotometer (Thermo Fisher Scientific, United States). Then, 1 μg of total RNA was reverse-transcribed into cDNA using oligo-dT primers. qRT-PCR was performed to analyze gene expression using SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biology, Hunan, China). Transcriptional levels of the target genes were normalized against that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Primers (Table 1) for target genes were designed using Oligo 7.0. Primer synthesis and qRT-PCR amplification reaction conditions were consistent with those reported in our previous study (Li et al., 2022 (link)).