Hiv 1 viral load amplification kit

A viral load quantification was performed using real-time polymerase chain reaction (PCR) amplification technology, the Sample Purific CV HIV-1 extraction kit (Abbott) and the HIV-1 viral load amplification kit (Abbott, Chicago, IL, USA). The viral load results were expressed as copies/mL and log10.

The viral load was quantified by real-time PCR using the Sample Purific CV HIV-1 extraction kit (Abbott) and the HIV-1 viral load amplification kit (Abbott, Chicago, Illinois, USA). The units used were copies/mL converted by log₁₀. Both the CD4⁺ and CD8⁺ T cells and the HIV-1 viral load were quantified according to the standard set by the National Network for the Determination of CD4⁺ and CD8⁺ T cells and Viral Load of the Department of HIV/AIDS and Viral Hepatitis of the Ministry of Health.

CD4+ TL and CD8+ TLs were quantified by flow cytometry (BD FACSCaliburTM, Becton & Dickinson, Franklin Lakes, NJ, USA) with the FACSCountTM Reagents monitoring kit, following the protocol recommended by the manufacturer (Becton & Dickinson, Franklin Lakes, NJ, USA). The viral load was quantified by real-time PCR using the Sample Purific CV HIV-1 extraction kit (Abbott, Chicago, IL, USA) and the HIV-1 viral load amplification kit (Abbott, Chicago, IL, USA). The units used were copies/mL and log10. The determinations followed the standard established by the National Network for the Determination of CD4+ and CD8+ T cells and Viral Load of the Department of HIV/AIDS and Viral Hepatitis of the Brazilian Ministry of Health. A statistical evaluation of the plasma levels of CD4+ and CD8+ TL was performed on the median values of each group.