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Transfection reagent 1

Manufactured by Horizon Discovery
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Sourced in United States

Transfection reagent 1 is a laboratory product designed for the delivery of nucleic acids, such as DNA or RNA, into cells. It facilitates the uptake of these molecules by the target cells, enabling researchers to study gene expression, gene function, and other cellular processes.

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Lab products found in correlation

Sirna duplexes, by Horizon Discovery (1 mentions) Sirna duplex, by Horizon Discovery (1 mentions) Opti mem medium, by Thermo Fisher Scientific (1 mentions) Mcdb131, by Thermo Fisher Scientific (1 mentions) Ccg 1423, by Santa Cruz Biotechnology (1 mentions) Live dead staining kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using transfection reagent 1

1

Knockdown of LKB1 using siRNA

Cited 1 time
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We followed shRNA methods described in our previous report.46 (link) For siRNA, a pool of four siRNA duplexes targeting human or mouse LKB1 and a nontargeting siRNA pool were purchased from Dharmacon, Inc. (Lafayette, CO, USA). Cells were grown in a six-well plate to 40% confluence and incubated with a mixture of a 60 nmol siRNA duplex and 5 μl of transfection reagent 1 (Dharmacon, Inc.). After 4 h, FBS was added to a final concentration of 10% (v/v). Additional transfection was performed at 48-h intervals later for some MEFs.
Werle K., Chen J., Xu H.G., Zhao R.X., He Q., Lu C., Cui R., Liang J., Li Y.L, & Xu Z.X. (2014). Liver kinase B1 regulates the centrosome via PLK1. Cell Death & Disease, 5(4), e1157-.
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2

Hsp40/DnaJB1 Knockdown in A549 Cells

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A549 cells were transfected with 100 nM control non-targeting siRNA pool (Mission siRNA Universal negative control 1, Sigma) or SMART pool siRNA against Hsp40/DnaJB1 (Dharmacon, CO, USA) using Transfection reagent 1 (Dharmacon). The siRNA transfection mix was made in Opti-MEM medium (Life Technologies). Serum containing DMEM medium was added to the cells 6 h p.t. Cells were infected 24 h post-siRNA transfection.
Batra J., Tripathi S., Kumar A., Katz J.M., Cox N.J., Lal R.B., Sambhara S, & Lal S.K. (2016). Human Heat shock protein 40 (Hsp40/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins. Scientific Reports, 6, 19063.
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3

Silencing MKL1, SRF, and Pfn1 in HmVEC Cells

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HmVEC-1, a widely used human dermal microvascular EC line (source: ATCC; Manassas, VA; CRL-3243 – referred to as HmVEC hereafter), were cultured in MCDB-131 (Life Technologies; Carlsbad, CA) growth medium [10% (v/v) fetal bovine serum, 100U/mL Penicillin, 100μg/mL Streptomycin, 10ng/mL EGF, 1μg/mL Hydrocortisone, 10mM L-Glutamine]. For silencing of genes, MKL1 siRNA (Santa Cruz, Dallas, TX, USA, sc-43944), SRF siRNA (Santa Cruz, sc-36563), or Pfn1 siRNA (GE Dharmacon, Lafayette, CO, M-012003-01-0005), all at 100 nM, were transfected using Transfection Reagent 1 (GE Dharmacon) following the manufacturer’s instructions. HmVEC were treated with CCG-1423 (Santa Cruz) for 24 hours under serum-starved conditions prior to extraction. Cell viability was assessed by a live-dead staining kit (Molecular Probes) according to the manufacturer’s instruction.
Gau D., Veon W., Capasso T.L., Bottcher R., Shroff S., Roman B.L, & Roy P. (2017). Pharmacological Intervention of MKL/SRF signaling by CCG-1423 impedes Endothelial Cell Migration and Angiogenesis. Angiogenesis, 20(4), 663-672.
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