Adenosine 5 triphosphate

The all DNA and RNA sequences synthesized
and purified with HPLC by Integrated DNA Technologies (UK) are listed
in Table S1. Cas12a and related buffers
were purchased from Integrated DNA Technologies. Adenosine 5′-triphosphate
(ATP), CTP, GTP, UTP, and RNase inhibitor were purchased from NEW
ENGLAND BioLabs Inc. (Ipswich, UK). NHS-Biotin was purchased from
Sigma-Aldrich, Inc. (H1759–100 MG). Polystreptavidin R buffer
was purchased from BioTeZ Berlin-Buch GmbH. 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES), with APTES, and other chemicals were purchased from
Sigma-Aldrich, Inc.

All antibodies used are listed in Table S7. Anti-MASTL rabbit polyclonal and phospho-specific antibodies against Ser875 were generated as previously described (Burgess et al., 2010 (link); Vigneron et al., 2011 (link)). The following chemicals were used: RO3306 (Axon MedChem), okadaic acid sodium salt (A.G. Scientifix), nocodazole (Sigma-Aldrich), thymidine 2′-deoxycytidine hydrate (Santa Cruz Biotechnology), protease inhibitor cocktail (PIC) (Sigma-Aldrich), adenosine 5′-triphosphate (New England Biolabs), (S)-MG132 (Cayman Chemicals), iodoacetamide (Sigma-Aldrich) and TCEP-hydrochloride (ThermoFisher Scientific). PDP3 peptide (Reither et al., 2013 (link)) was synthesised using H-Rink Amide ChemMatrix resin at a loading concentration of 0.52 mmol/g. Automated and manual solid-phase peptide synthesis (SPPS), preparative and analytical high-performance liquid chromatography and analytical LC-MS was performed as described previously (Mitchell et al., 2015 (link)).