Immunohistochemistry was performed on all cases, either at the time of original diagnosis or for the purposes this study. Whole-slide sections of formalin-fixed, paraffin embedded tumor tissue were cut at five-micron thickness, deparaffinized, and subjected to antigen retrieval using 10 mM citrate buffer at 92°C for 30 minutes. Immunohistochemistry was performed on all cases using antibodies for S100 (Ventana Medical Systems, Tucson, AZ), either Smooth Muscle Actin (Ventana) or Calponin (Dako, Carpinteria, CA), SOX10 (BioCare Medical, Concord, CA), Desmin (Dako), and β-catenin (BD Biosciences, San Jose, CA). In addition, for a subset of cases (n=6), PAX3 (Bioss Inc., Wolburn, MA) immunohistochemistry was also performed. Depending on tissue availability, immunohistochemistry was also performed in most cases using antibodies for myogenin (Ventana) and Factor XIIIa (Cell Marque, Rocklin, CA). All immunohistochemical signals were visualized using the Ultra view polymer detection kit (Ventana Medical Systems, Inc. Tucson, AZ) on a Ventana BenchmarkXT autostainer (Ventana). Staining was performed according to manufacturer's instructions in the presence of appropriate controls. “Focal” immunoreactivity was regarded as immunostaining in <10% of tumor cells.
Sox10
Manufactured by Biocare Medical
SOX10 is a DNA-binding transcription factor that plays a role in the development and differentiation of neural crest cells. It is commonly used as a biomarker for the identification and characterization of melanocytic and schwannian cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Desmin, by Agilent Technologies (1 mentions)
Myogenin, by Roche (1 mentions)
Factor xiiia, by Cell Marque (1 mentions)
Ultra view polymer detection kit, by Roche (1 mentions)
Calponin, by Agilent Technologies (1 mentions)
Ventana benchmark xt, by Roche (1 mentions)
β catenin, by BD (1 mentions)
Ph 9 buffer, by PerkinElmer (1 mentions)
Hardset mounting media, by Vector Laboratories (1 mentions)
Dako autostainer plus, by Agilent Technologies (1 mentions)
Pd l1, by Cell Signaling Technology (1 mentions)
2 protocols using sox10
1
Immunohistochemical Profiling of Rare Tumors
2
Multiplex Immunofluorescence for Tumor Immune Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Multiplex immunofluorescence was performed on baseline FFPE specimens, as previously described.13 Briefly, 4µM FFPE sections were deparaffinized in xylene and subsequently run through graded ethanols. Antigen retrieval was performed using pH 9 buffer (Perkin Elmer) in a microwave, and then cooled on the bench in TBST before commencing staining using the DAKO Autostainer Plus. Slides were sequentially stained with the primary antibodies for PD-1 (Cell Marque, 1:500), FOXP3 (Abcam, 1:2000), SOX10 (Biocare Medical, 1:800), PD-L1 (Cell Signaling Technology, 1:2000), and CD8 (Sigma-Aldrich, 1:500). Antibodies were detected using the MACH3 HRP-Polymer detection kits, and visualized using the Opal TSA fluorophores (Perkin Elmer; 1:50). Following all stains, sections were counterstained with DAPI and slides were coverslipped using the VectaShield Hardset mounting media.
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