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Ab6081

Manufactured by Abcam
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Sourced in Germany

Ab6081 is a rabbit monoclonal antibody that recognizes the human CD3 epsilon subunit. CD3 is a protein complex that is involved in T cell activation and signal transduction.

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Lab products found in correlation

Ab168381, by Abcam (1 mentions) Sc 1657, by Santa Cruz Biotechnology (1 mentions) Irdye 800cw donkey anti rabbit, by LI COR (1 mentions) Ab51109, by Abcam (1 mentions) Irdye 680rd donkey anti mouse, by LI COR (1 mentions)

2 protocols using ab6081

1

Quantifying 14-3-3 Isoform Phosphorylation

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Primary antibodies included rabbit polyclonal antibody against pan 14‐3‐3 isoforms (Abcam #ab6081, Cambridge, MA), mouse monoclonal antibody against pan 14‐3‐3 isoforms (Santa Cruz #sc‐1657, Dallas, TX), mouse monoclonal antibody against 14‐3‐3θ (Abcam #ab10439), rabbit polyclonal antibody against phospho‐S58 (pS58) 14‐3‐3 (Abcam #ab51109), rabbit polyclonal antibody against phospho‐S232 (pS232) 14‐3‐3θ (Abcam #ab63369), sheep polyclonal antibody against phospho‐S185 (pS185) 14‐3‐3 (ENZO Life Science #SA‐479, Farmingdale, NY), and rabbit monoclonal antibody against phospho‐S129 αsyn (Abcam #ab168381). Horseradish peroxidase‐conjugated secondary antibodies were goat anti‐rabbit and goat anti‐sheep (Jackson Immunoresearch, West Grove, PA). Fluorescent‐conjugated secondary antibodies used were IRDye 800CW donkey anti‐rabbit and IRDye 680RD donkey anti‐mouse (LI‐COR Biotechnology, Lincoln, NE).
McFerrin M.B., Chi X., Cutter G, & Yacoubian T.A. (2017). Dysregulation of 14‐3‐3 proteins in neurodegenerative diseases with Lewy body or Alzheimer pathology. Annals of Clinical and Translational Neurology, 4(7), 466-477.
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2

Immunofluorescence analysis of phosphorylated HDAC5, TBC1D1, and 14-3-3

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Cells were fixed with 4% paraformaldehyde (PFA)/PBS at room temperature for 15 min. After blocking with 3% bovine serum albumin (BSA) at room temperature for 30 min, the fixed cells were incubated with primary anti‐p‐HDAC5 (1 : 500, gtx50238; Gene Tex, Irvine, CA, USA), primary anti‐p‐TBC1D1 (1 : 500, 07‐2268; Merck Milipore, Damstadt, Germany), or primary anti‐14‐3‐3 (1 : 500, ab6081; Abcam) antibodies in primary antibody diluent (PBS, 3% BSA, and 0.1% Triton X‐100) at 4 °C overnight. Cells were then washed with PBS, probed with a goat anti‐rabbit Cy3 (red) or goat anti‐mouse 488 (green) secondary antibody (Molecular Probes, Eugene, OR, USA) and washed three times with PBS at room temperature for 10 min. Images were obtained using a Zeiss confocal microscope (LSM700).
Lee J.O., Byun W.S., Kang M.J., Han J.A., Moon J., Shin M., Lee H.J., Chung J.H., Lee J., Son C., Song K., Kim T.W., Lee E., Kim H.M., Chung C.H., Ngoei K.R., Ling N.X., Oakhill J.S., Galic S., Murray‐Segal L., Kemp B.E., Kim K.M., Lim S, & Kim H.S. (2020). The myokine meteorin‐like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPKα2. The Febs Journal, 287(10), 2087-2104.
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