Log In Sign up
The largest database of trusted experimental protocols

Pd 1 eh12.1

Manufactured by BioLegend
Visit Supplier
Sourced in Denmark

PD-1 (EH12.1) is a monoclonal antibody that specifically binds to the Programmed Cell Death 1 (PD-1) receptor. PD-1 is an immune checkpoint protein expressed on the surface of activated T cells, B cells, and myeloid cells. The core function of this antibody is to enable the detection and analysis of PD-1 expression on cells.

Automatically generated - may contain errors

Lab products found in correlation

Live dead fixable dead cell stain, by Thermo Fisher Scientific (2 mentions) Cd45ra hi100, by BioLegend (2 mentions) Granzyme b gb11, by BioLegend (2 mentions) Cd8 sk1 or rpa t8, by BioLegend (2 mentions) Ccr7 g043h7, by BioLegend (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Facsaria 2, by BD (2 mentions) Facsymphony, by BD (2 mentions) Gzmb gb11, by BioLegend (1 mentions) Cd4 pe cy7 sk3, by BioLegend (1 mentions) Live dead near ir dye, by Thermo Fisher Scientific (1 mentions) Cd38 bv605 hb7, by BioLegend (1 mentions) Cd8 percp cy5, by BioLegend (1 mentions) Hla dr, by BD (1 mentions)

Close spelling variants of this product

Anti-PD-1 antibody (clone EH12.2H7) Human PD-1 (clone EH12.2H7, PD-1 (clone EH12.2H7), PD‐1 BV421 (EH12.2H7; Anti-human PD-1 (clone EH12.2H7, Anti-PD-1 PerCP-Cy5.5 (EH12.2H7, Anti-PD-1 BV421 (clone EH12.2H7), Anti-PD-1 (clone EH12.2H7, Anti-PD-1 (EH12.2H7, PE anti-human CD279 (PD-1) (EH12.2H7,

4 protocols using pd 1 eh12.1

1

Multiplex Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
GzmB (GB11), PD-1 (EH12.1) and TIM-3(7D3) all Biolegend.
Phetsouphanh C., Phalora P., Hackstein C.P., Thornhill J., Munier C.M., Meyerowitz J., Murray L., VanVuuren C., Goedhals D., Drexhage L., Russell R.A., Sattentau Q.J., Mak J.Y., Fairlie D.P., Fidler S., Kelleher A.D., Frater J, & Klenerman P. (2021). Human MAIT cells respond to and suppress HIV-1. eLife, 10, e50324.
+ Open protocol
+ Expand
2

T Cell Phenotyping of Frozen PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen PBMC’s were thawed and 5x105 cells were immediately stained with Near-IR live-dead dye (LifeTechnologies, Denmark), blocked and then stained with antibodies to CD4-PE-Cy7 (SK3), CD8+-PerCP-Cy5.5 (SK1), CD45RA (HI100), CCR7 (G043H7) CD69-APC (FN50), HLA-DR-PE (G46-6) and CD38-BV605 (HB7) or PD-1 (EH12.1) (all Biolegend except PD-1, CD38, HLA-DR and CD8+; BD Bioscience). Only singlet, live cells were included in the data analyses. T cells were gated based upon size and granularity (lymphocyte gate). Within the lymphocyte gate T cells were sub-gated based upon their expression of either CD4 or CD8+. Memory subsets within CD4+ and CD8+ T cells were defined based on CCR7 and CD45RA expression. Activation status was determined based upon CD69 expression or HLA-DR/CD38 co-expression. Gates for activation markers and PD-1 were determined using isotope control antibodies.
Søgaard O.S., Graversen M.E., Leth S., Olesen R., Brinkmann C.R., Nissen S.K., Kjaer A.S., Schleimann M.H., Denton P.W., Hey-Cunningham W.J., Koelsch K.K., Pantaleo G., Krogsgaard K., Sommerfelt M., Fromentin R., Chomont N., Rasmussen T.A., Østergaard L, & Tolstrup M. (2015). The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo. PLoS Pathogens, 11(9), e1005142.
+ Open protocol
+ Expand
3

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were washed with PBS and stained with LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher) for 15 minutes, followed by staining with an optimized antibody cocktail for 20 minutes at room temperature (RT). Cells were then washed with FACS buffer (PBS containing 2% FBS) and fixed in PBS containing 1% paraformaldehyde (Sigma-Aldrich). For samples requiring intracellular staining, cells were processed using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher) according to the manufacture’s instruction. Samples were acquired using a FACSymphony (BD Biosciences). Data were analyzed with FlowJo software (version 9.8.8 or higher, BD Biosciences). All sorting experiments were carried out on freshly isolated cells using a FACSAriaII (BD Biosciences). Antibodies used include: CD45 (HI30), CD103 (Ber-ACT8), CD45RA (HI100, Biolegend), CD3 (UCHT1), CD69 (FN50), CD8 (SK1 or RPA-T8), CCR7 (G043H7, Biolegend), CD25 (M-A251), CD127 (HIL-7R-M21), CD4 (RPA-T4), CCR5 (2D7), PD-1 (EH12.1, Biolegend), Granzyme B (GB11), Foxp3 (259D/C7), CTLA4 (BNI3), IL-17A (N49-653), IL-2 (5344.111), (TNFα (Mab11), IFNγ (B27). All antibodies were from BD Biosciences unless otherwise noted.
Davis A.S., Vick S.C., Pattacini L., Voillet V., Hughes S.M., Lentz G.M., Kirby A.C., Fialkow M.F., Gottardo R., Hladik F., Lund J.M, & Prlic M. (2021). The human memory T cell compartment changes across tissues of the female reproductive tract. Mucosal immunology, 14(4), 862-872.
+ Open protocol
+ Expand
4

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were washed with PBS and stained with LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher) for 15 minutes, followed by staining with an optimized antibody cocktail for 20 minutes at room temperature (RT). Cells were then washed with FACS buffer (PBS containing 2% FBS) and fixed in PBS containing 1% paraformaldehyde (Sigma-Aldrich). For samples requiring intracellular staining, cells were processed using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher) according to the manufacture’s instruction. Samples were acquired using a FACSymphony (BD Biosciences). Data were analyzed with FlowJo software (version 9.8.8 or higher, BD Biosciences). All sorting experiments were carried out on freshly isolated cells using a FACSAriaII (BD Biosciences). Antibodies used include: CD45 (HI30), CD103 (Ber-ACT8), CD45RA (HI100, Biolegend), CD3 (UCHT1), CD69 (FN50), CD8 (SK1 or RPA-T8), CCR7 (G043H7, Biolegend), CD25 (M-A251), CD127 (HIL-7R-M21), CD4 (RPA-T4), CCR5 (2D7), PD-1 (EH12.1, Biolegend), Granzyme B (GB11), Foxp3 (259D/C7), CTLA4 (BNI3), IL-17A (N49-653), IL-2 (5344.111), (TNFα (Mab11), IFNγ (B27). All antibodies were from BD Biosciences unless otherwise noted.
Davis A.S., Vick S.C., Pattacini L., Voillet V., Hughes S.M., Lentz G.M., Kirby A.C., Fialkow M.F., Gottardo R., Hladik F., Lund J.M, & Prlic M. (2021). The human memory T cell compartment changes across tissues of the female reproductive tract. Mucosal immunology, 14(4), 862-872.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!