Ab46087

Manufactured by Abcam

Sourced in United Kingdom, United States, Netherlands

Ab46087 is a laboratory equipment product. It is a piece of lab equipment designed for a specific function in scientific research and analysis. No further details about its core function or intended use can be provided in an unbiased and concise manner.

Automatically generated - may contain errors

Lab products found in correlation

Ab46027, by Abcam (3 mentions) Ab46032, by Abcam (2 mentions) Ab214025, by Abcam (2 mentions) Ab46063, by Abcam (2 mentions) Ab100562, by Abcam (1 mentions) 24 well plate, by Corning (1 mentions) Ab46052, by Abcam (1 mentions) Hcytomag 60k, by Merck Group (1 mentions) Khc0061, by Thermo Fisher Scientific (1 mentions) Kac1591, by Thermo Fisher Scientific (1 mentions) Dtslp0, by R&D Systems (1 mentions) Ab46042, by Abcam (1 mentions) Ab46048, by Abcam (1 mentions) Ab46034, by Abcam (1 mentions)

6 protocols using ab46087

Measuring Inflammatory Cytokines in LPS-Stimulated Chondrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary chondrocyte cells were seeded into 24‐well plates (Corning) for 24 h. After LPS stimulation, the culture supernatant was collected for estimation of inflammatory factors using commercial enzyme‐linked immunosorbent assay (ELISA) kits in accordance with the manufacturer's instructions. The ELISA kits, including a human IL‐1β kit (ab100562), a human IL‐6 kit (ab46027), a human IL‐8 kit (ab46032), and a human TNF‐α kit (ab46087), were purchased from Abcam (Cambridge, UK). The OD values at 450 nm were read, and the levels of IL‐1β, IL‐6, IL‐8, and TNF‐α were determined according to a representative standard curve of OD/quantity (pg/mL).

Li X., Wei X., Wang S., Sun G., Zhao Y., Yin H., Li L., Yin X., Li K., Zhu L, & Zhang H. (2020). Differentiation Antagonizing Non‐protein Coding RNA Knockdown Alleviates Lipopolysaccharide‐Induced Inflammatory Injury and Apoptosis in Human Chondrocyte Primary Chondrocyte Cells Through Upregulating miRNA‐19a‐3p. Orthopaedic Surgery, 13(1), 276-284.

+ Open protocol

+ Expand

Cytokine Secretion Profiling by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ELISA assays in the supernatant of cell culture were performed to measure the secreted cytokines including IL-1β, IL-6, TNF-α, and IL-4 following a previous protocol 42 (link). Briefly, the CtBP1-OE and CtBP2-OE cells were seeded into 6-well plates and cultured at 37°C for 48 h. The medium was collected and centrifuged at 1500 rpm for 10 min and the supernatant was applied to ELISA assays using different kits including IL-1β (Αbcam, #ab214025), IL-6 (Abcam, #ab46027), TNF-α (Αbcam, #ab46087), and IL-4 (Abcam, # ab46063) following the protocols provided by the manufacturer.

Sun X., Xiao L., Chen J., Chen X., Chen X., Yao S., Li H., Zhao G, & Ma J. (2020). DNA methylation is involved in the pathogenesis of osteoarthritis by regulating CtBP expression and CtBP-mediated signaling. International Journal of Biological Sciences, 16(6), 994-1009.

+ Open protocol

+ Expand

Cytokine Profiling in Cell-Free Sputum Supernatants

Check if the same lab product or an alternative is used in the 5 most similar protocols

In cell-free sputum supernatants, the following cytokines were measured with ELISA kits according to the manufacturer's instructions: IL-1β (ab46052; Abcam, Cambridge, UK), IL-5 (ab46026; Abcam), IL-6 (KHC0061; Invitrogen, Camarillo, CA, USA), IL-8 (ab46032; Abcam), IL-13 (ab46038; Abcam), IL-17 (KAC1591; Invitrogen), IL-25 (EK0793; Boster Biotech; Pleasanton, CA, USA), IL-33 (SEB980Hu; Cloud-Clone Corp., Katy, TX, USA), TNF-α (ab46087; Abcam), TSLP (DTSLP0; R&D Systems, Minneapolis, MN, USA), GM-CSF (HCYTOMAG-60K; EMD Millipore, Darmstadt, Germany), and IFN-γ (HCYTOMAG-60K; EMD Millipore). Cytokine levels were normalized to protein concentrations in the sputum.

Son J.H., Kim J.H., Chang H.S., Park J.S, & Park C.S. (2020). Relationship of Microbial Profile With Airway Immune Response in Eosinophilic or Neutrophilic Inflammation of Asthmatics. Allergy, Asthma & Immunology Research, 12(3), 412-429.

+ Open protocol

+ Expand

Serum Cytokine Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All subjects were collected 3–5 mL of venous blood on an empty stomach after treatment. The collected blood was allowed to stand at room temperature for 30 min, centrifuged at 3000 rpm for 15 min. Then the serum was collected and stored at −80°C for standby. According to the manufacturer's instructions, the serum TNF‐α, IL‐6, and IFN‐γ levels were determined by TNF‐α (ab46087, Abcam), IL‐6 (ab46042), and IFN‐γ (ab46048) ELISA kits.

Maowulieti G., Zhao S., Zhao M, & Yuan H. (2023). The role of inflammatory factors and T‐cell subsets in the diagnosis of recurrence in epithelial ovarian cancer patients and the effect of olaparin treatment on them. Immunity, Inflammation and Disease, 11(10), e1059.

+ Open protocol

+ Expand

Metabolic and Inflammatory Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The venous blood sample was obtained on the day of the biopsy after an overnight fast of at least 10 h. Biochemical measurements include fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined with an autoanalyzer (Hitachi 917, Boehringer Mannheim, Marburg, Germany) using colorimetric assay methods. In addition, serum insulin (Cat. No: 2425-300 A, AccuBind, Netherlands), TNFα (ab46087, Abcam), and IL10 (ab46034, Abcam) were measured by Human ELISA kits. The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated using the following formula: Fasting insulin (in mU/ml) × Fasting glucose (in mmol/l) /22.5.

Pourdashti S., Faridi N., Monem-Homaie F., Yaghooti S.H., Soroush A, & Bathaie S.Z. (2023). The size of human subcutaneous adipocytes, but not adiposity, is associated with inflammation, endoplasmic reticulum stress, and insulin resistance markers. Molecular Biology Reports, 50(7), 5755-5765.

+ Open protocol

+ Expand

Cytokine Quantification in CRC Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

The enzyme-linked immunosorbent assays (ELISAs) were performed following a previous protocol. 11 Briefly, blood samples from 24 CRC patients (same patients as tissue collection) and 24 controls (same volunteers as the previous study 11 ) were applied to ELISAs to measure the concentrations of cytokines, including IL-1b, IL-4, IL-6, and TNFa. The ELISA kits were purchased from Abcam Company (Cambridge, MA), and they included ab214025 (IL-1b), ab46063 (IL-4), ab46027 (IL-6), and ab46087 (TNF-a). In addition, the ELISAs were also performed to measure the concentrations of IL-6 and TNF-a in the supernatant of cell culture medium of p65-overexpression cells.

Yang C., Lu W., He H, & Liu H. (2020). Inflammation and DNA Methylation-Dependent Down-Regulation of miR-34b-5p Mediates c-MYC Expression and CRL4^DCAF4 E3 Ligase Activity in Colitis-Associated Cancer. The American journal of pathology, 190(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!