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Anti tnfsf10

Manufactured by Cell Signaling Technology
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Anti-TNFSF10 is a reagent used for the detection and quantification of TNFSF10, also known as TRAIL, a member of the tumor necrosis factor superfamily. It can be used in various immunoassay techniques to measure TNFSF10 levels in biological samples.

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Lab products found in correlation

Anti stat1, by Cell Signaling Technology (1 mentions) Anti tubulin, by Cell Signaling Technology (1 mentions) Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions) Odyssey classic infrared imaging system, by LI COR (1 mentions) Anti β actin, by Abcam (1 mentions)

2 protocols using anti tnfsf10

1

Antibody Source and Protocols

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The antibodies used in this study were purchased from the following sources: anti-KRT18 (#4548), anti-STAT1 (#9172), and anti-TNFSF10 (#3219) were from Cell Signaling (Danvers, MA, USA); anti-Fn1 (AB2033) was from Millipore (Billerica, MA, USA).
Ko Y.C., Lai T.Y., Hsu S.C., Wang F.H., Su S.Y., Chen Y.L., Tsai M.L., Wu C.C., Hsiao J.R., Chang J.Y., Wu Y.M., Robinson D.R., Lin C.Y, & Lin S.F. (2020). Index of Cancer-Associated Fibroblasts Is Superior to the Epithelial–Mesenchymal Transition Score in Prognosis Prediction. Cancers, 12(7), 1718.
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2

Quantitative PCR and Western Blot Analysis

Cited 1 time
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qRT-PCR was performed using the TaqMan Gene Expression Master Mix (Thermo Fisher Scientific, #4369016), along with TaqMan Assays for TNFSF10 (Thermo Fisher Scientific, Hs00921974_m1), DDX58 (IDT, Hs.PT.58.4273674), IFIH1 (IDT, Hs.PT.58.1224165), OAS1 (IDT, Hs.PT.58.2338899), TNF (IDT, Hs.PT.5845380900) or CXCL8 (IDT, Hs.PT.5839926886). The fold change in expression of each gene was calculated using the ΔΔCT method, with RNA18S (IDT, Hs.PT.39a.22214856.g) as an internal control. To detect protein expression of TNFSF10, we conducted western blot analyses as previously described (36 (link)), using anti-TNFSF10 (Cell Signaling, #3219, 1:300 dilution), anti-tubulin (Cell Signaling, #2128, 1:2000 dilution) and anti-β-actin (abcam, #ab8227, 1:10 000 dilution) antibodies. After incubating with a fluorescent secondary antibody (Li-Cor, #926–68 071, 1:20 000 dilution), the fluorescence signals were detected by Li-Cor Odyssey Classic Infrared Imaging System, according to the manufacturer’s protocol.
Han Y.J., Zhang J., Hardeman A., Liu M., Karginova O., Romero R., Khramtsova G.F., Zheng Y., Huo D, & Olopade O.I. (2022). An enhancer variant associated with breast cancer susceptibility in Black women regulates TNFSF10 expression and antitumor immunity in triple-negative breast cancer. Human Molecular Genetics, 32(1), 139-150.
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