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Histo

Manufactured by Electron Microscopy Sciences
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The Histo is a versatile laboratory equipment designed for the preparation and processing of biological samples for examination under a microscope. It serves as a core tool for histological analysis, providing essential functionalities for tissue processing, embedding, and sectioning. The Histo offers reliable and consistent results, enabling researchers and technicians to effectively prepare specimens for detailed microscopic investigation.

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Lab products found in correlation

Rm2265, by Leica (2 mentions) Entellan, by Merck Group (2 mentions) Ultracut r, by Electron Microscopy Sciences (2 mentions) Ultra afm, by Electron Microscopy Sciences (2 mentions) Epofix, by Struers (2 mentions) Cellsens, by Olympus (1 mentions)

5 protocols using histo

1

Semi-Thin Plastic Sections Staining

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For light-microscopical assessment, semi-thin (1 μm) plastic sections were cut with a diamond knife (Histo, DiATOME) on a microtome (RM2265, Leica Microsystems), collected on aminoalkylsilane-coated glass slides, stained with a warm aqueous solution containing 1% methylene blue, 1% azure II and 1% sodium tetraborate, and mounted in Entellan (1079610100, Merck) under a glass coverslip.
Valentin H.E., Mitsky T.A., Mahadeo D.A., Tran M, & Gruys K.J. (2000). Application of a Propionyl Coenzyme A Synthetase for Poly(3-Hydroxypropionate-co-3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli. Applied and Environmental Microbiology, 66(12), 5253-5258.
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2

Quantifying Optic Nerve Axons in Mice

Cited 1 time
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8-week-old mice were euthanized by carbon dioxide inhalation with death confirmed by cervical spine dislocation. Optic nerves were collected, processed for Histology, stained, imaged, and quantified as previously described (Mao et al., 2011 (link); Trantow et al., 2009 (link)). Mouse heads were fixed in half-strength Karnovsky’s fixative for a minimum of 24 hours. Nerves were then dissected and embedded in resin. Histologic sections were cut using an ultramicrotome (UC6, Leica, Wetzler, German) equipped with a diamond knife (Histo, Diatome, Hatfield, PA, USA), stained with paraphenylenediamine (PPD; (Anderson et al., 2005 (link))), and imaged using a light microscope (BX52; Olympus, Tokyo, Japan) equipped with a camera (DP72; Olympus, Tokyo, Japan) and corresponding software (CellSens; Olympus, Tokyo, Japan). Cross-sectional area of the optic nerve was measured using ImageJ. Axons were counted from 6–10 non-overlapping fields at 1000X magnification representing 10% of the optic nerve cross-sectional area. The total number of axons for each nerve was estimated by multiplying the sum of the axons in the counted fields by 10.
van der Heide C.J., Meyer K.J., Hedberg-Buenz A., Pellack D., Pomernackas N., Mercer H.E, & Anderson M.G. (2021). Quantification and image-derived phenotyping of retinal ganglion cell nuclei in the nee mouse model of congenital glaucoma. Experimental eye research, 212, 108774.
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3

Semi-Thin Plastic Sections Staining

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For light-microscopical assessment, semi-thin (1 μm) plastic sections were cut with a diamond knife (Histo, DiATOME) on a microtome (RM2265, Leica Microsystems), collected on aminoalkylsilane-coated glass slides, stained with a warm aqueous solution containing 1% methylene blue, 1% azure II and 1% sodium tetraborate, and mounted in Entellan (1079610100, Merck) under a glass coverslip.
van der Valk W.H., van Beelen E.S., Steinhart M.R., Nist-Lund C., Osorio D., de Groot J.C., Sun L., van Benthem P.P., Koehler K.R, & Locher H. (2023). A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs. Cell reports, 42(6), 112623.
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4

Dehydration and Embedding for Nanoscale Characterization

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A series of ethanol/deionized water concentration (50%, 75%, 90% and 100%) were used to dehydrate samples. After this step, to maintain the fibre and the cell-wall structure during surface preparation, samples were embedded in a mixture containing increasing proportions of LR (London Resin) White acrylic resin/ethanol (25%, 50%, 75% and 100%). Then, the polymerization of the resin was finalised in an oven at 60 °C for one night. The aramid fibres were introduced into an epoxy resin (Struers Epofix) for 24 h and polymerization took place at room temperature. To obtain reliable measurements with the atomic force microscope, we must minimize their roughness at the nanometer scale. Consequently, the samples were prepared using an ultramicrotome (Leica Ultracut R) with two consecutive diamond knives (Diatome Histo and Ultra AFM), to cut very thin sections (about 50 nm) at low cutting speed (≈1 mm/s) and thus reduce sample deformations.
Bourmaud A., Siniscalco D., Foucat L., Goudenhooft C., Falourd X., Pontoire B., Arnould O., Beaugrand J, & Baley C. (2019). Evolution of flax cell wall ultrastructure and mechanical properties during the retting step. Carbohydrate polymers, 206.
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5

Preparation of Flax and Aramid Fiber Samples

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Green stems and mature flax fibres samples were dehydrated with a concentration series of ethanol/deionised water (50%, 75%, 90% and 100%) and then embedded in a mixture containing increasing ratios of LR White acrylic resin/ethanol (25%, 50%, 75% and 100%), waiting for few hours at each embedding step, in order to maintain the fibre and the cell-wall structure during surface preparation. Final resin polymerization took place in an oven (60 • C, overnight). Aramid fibres were placed directly in an epoxy resin (Struers Epofix) that polymerizes at ambient temperature for 24 h. One stem sample and one sample containing several tens of mature flax fibres and another one containing several tens of aramid fibres were prepared. They were then machined, to reduce their crosssection, and an ultramicrotome (Leica Ultracut R) is then used with diamond knives (Diatome Histo and Ultra AFM) to cut a series of very thin sections (about 50 nm thick in the last steps) at low cutting speed (≈1 mm/s).
Arnould O., Siniscalco D., Bourmaud A., Le Duigou A, & Baley C. (2017). Better insight into the nano-mechanical properties of flax fibre cell walls. Industrial Crops & Products., 97.
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