Wincycle software

Manufactured by Phoenix Pharmaceuticals

Sourced in United States

WinCycle is a software application designed for use in scientific laboratories. It provides a platform for managing and analyzing data generated from various laboratory equipment and instrumentation.

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Lab products found in correlation

Facscalibur, by BD (2 mentions) Cell cycle analysis kit, by Beyotime (1 mentions) Flow cytometry, by BD (1 mentions) Kaluza flow cytometry analysis software 1, by Beckman Coulter (1 mentions) Facscanto 2, by BD (1 mentions) Propidium iodide flow cytometry assay kit, by Abcam (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Epics xl flow cytometer, by Beckman Coulter (1 mentions) Propidium iodide, by Merck Group (1 mentions) Rnase a, by Merck Group (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions)

Spelling variants of this product

MultiCycle software WinCycle 32 (2 citations)

7 protocols using wincycle software

Flow cytometry analysis of apoptosis and cell cycle

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flow cytometry in analysis of apoptosis was performed using a double staining method with Annexin-V-FITC and propidium iodide (PI). The assay was carried out using Apoptosis Kit (KeyGen Biotech. Co. Ltd., Nanjing, China) according to the manufacturer’s instruction. Cells were cultured with 2DG and/or insulin for 24h and then trypsinized without EDTA, washed with PBS, stained with Annexin V-FITC and PI and analyzed by flow cytometry (Becton-Dickinson, San Jose, CA). Data were processed by Kaluza flow cytometry analysis software 1.2 (Beckman Coulter).
For cell cycle analysis, Cell Cycle Analysis Kit from Beyotime Biotechnology (Shanghai, China) was used based on the manufacturer’s instruction. Cells were collected, washed with PBS, fixed in 75% cold ethanol and incubated overnight at 4°C. Then cells were washed and re-suspended in staining solution containing PI and RNAseA and incubated for 30 minutes at 37°C before flow cytometry analysis. Cell cycle fractions were quantified with WinCycle software (Phoenix Flow Systems, San Diego, CA).

Zhang D., Fei Q., Li J., Zhang C., Sun Y., Zhu C., Wang F, & Sun Y. (2016). 2-Deoxyglucose Reverses the Promoting Effect of Insulin on Colorectal Cancer Cells In Vitro. PLoS ONE, 11(3), e0151115.

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Cell Cycle Analysis by Flow Cytometry

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Cells were washed and trypsinized. The cell suspensions were recovered and resuspended in the following fixing solution: 7 ml 1× phosphate buffer saline, 2% bovine serum albumin, 5mM EDTA, 0.1% NaN₃. 3 ml of 100% ethanol was added drop wise. Fixed cells were centrifuged, washed and then resuspended in 500µl sorting buffer: 1× phosphate buffered saline, 0.1% Triton-X 100, 2% bovine serum albumin, 5mM EDTA, 40µg/ml propidium iodide, 100µg/ml RNAse A, and incubated at 37C for 30 min. The cells were filtered through 70 micrometer mesh to remove all cell aggregates. The DNA content was analyzed by flow cytometry (FACSCalibur; Becton Dickinson), and percentages of cells within each phase of the cell cycle was determined using Win Cycle software (Phoenix Flow Systems).

Chatterjee A., Mukhopadhyay S., Tung K., Patel D, & Foster D.A. (2015). Rapamycin-induced G1 cell cycle arrest employs both TGF-β and Rb pathways. Cancer letters, 360(2), 134-140.

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Cell Cycle Analysis by Flow Cytometry

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Cells cycle analysis were using Propidium Iodide Flow Cytometry Assay Kit (Abcam, USA), Briefly, about 1 × 10⁵ cells/ml of CSC cells from younger and older mice were seeded into 24 well plates and kept under 37°C, 5% CO₂overnight. Fixation of the cells was carried out using 66% ethanol for at least 2 h at 4°C. After centrifugation at 500 × g for 5 min, the cells were washed with PBS prior to staining. About 200 μl of staining solution composed of 1 × PI and RNase was added to the cell suspension and incubated in dark at 37°C for 20–30 min. The signals of at least 1×10⁴single cells were recorded on a FACS Canto II flow cytometer (BD Biosciences, Heidelberg, Germany), and DNA histograms were analyzed with the WinCycle software (Phoenix Flow Systems, San Diego, CA, USA) after doublet exclusion.

Wu Q., Zhan J., Pu S., Qin L., Li Y, & Zhou Z. (2016). Influence of aging on the activity of mice Sca-1+CD31− cardiac stem cells. Oncotarget, 8(1), 29-41.

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Cell Cycle Analysis by Flow Cytometry

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Briefly, Cells were trypsinized, washed with 1×PBS, and then fixed with 70% ethanol for overnight at 4°C. Following centrifugation, 500g for 5 min at 4°C, the fixed cells were washed with 1×PBS, and resuspended with 500ul of propidium iodide (PI) staining solution (0.2% Triton X-100, 100 μg/ml RNase, 50 μg/ml PI in 10 ml of PBS) and incubated in the dark for 30 min at room temperature. Flow cytometry analysis was performed on a BD FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA), and the proportion of cells in the G0/G1, S, and G2/M phases of the cell cycle were modeled using Wincycle software (Phoenix Flow Systems, San Diego, CA, USA).

Chen Y.N., Cai M.Y., Xu S., Meng M., Ren X., Yang J.W., Dong Y.Q., Liu X., Yang J.M, & Xiong X.D. (2016). Identification of the lncRNA, AK156230, as a novel regulator of cellular senescence in mouse embryonic fibroblasts. Oncotarget, 7(33), 52673-52684.

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Cell Cycle Analysis by Flow Cytometry

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Cells were washed and collected by trypsinazation. Recovered cells were re-suspended in a solution containing 7 ml of 2% BSA in phosphate buffered saline, 5mM EDTA, 0.1% NaN₃ and fixed by drop wise addition of 3ml of 100% ethanol. Fixed cells were collected and re-suspended in 500 μl of sorting buffer containing 2% BSA in phosphate buffered saline, 0.1% Triton-X 100, 5mM EDTA, 40μg/ml propidium iodide, 100μg/ml RNAse A, and incubated at 37C for 30 min. The cells were filtered through 70μm mesh to remove cell aggregates. The DNA content was analyzed by flow cytometry (FACSCalibur; Becton Dickinson), and percentages of cells within each phase of the cell cycle were determined using WinCycle software (Phoenix Flow Systems).

Salloum D., Mukhopadhyay S., Tung K., Polonetskaya A, & Foster D.A. (2014). Mutant Ras Elevates Dependence on Serum Lipids and Creates a Synthetic Lethality for Rapamycin. Molecular cancer therapeutics, 13(3), 733-741.

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Cell cycle analysis of AH-130 cells

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The DNA distribution/cell cycle analysis was performed in AH‐130 cells obtained from alternate day paracentesis in TB rats. Briefly, cells were washed in phosphate buffer solution (PBS), fixed in ice‐cold 70% ethanol for at least 30 min, incubated at room temperature in PBS containing DNase‐free RNase and propidium iodide at the final concentrations of 0.4 mg/mL and 10 µg/mL, respectively. Cells were then analysed using a Beckman Coulter Epics XL flow cytometer. Data were then analysed with the WinCycle software (Phoenix Flow Systems, San Diego, CA, USA). The percentage of apoptotic cells was assessed by evaluating the accumulation of cells having a <2n DNA content.

Toledo M., Penna F., Oliva F., Luque M., Betancourt A., Marmonti E., López‐Soriano F.J., Argilés J.M, & Busquets S. (2015). A multifactorial anti‐cachectic approach for cancer cachexia in a rat model undergoing chemotherapy. Journal of Cachexia, Sarcopenia and Muscle, 7(1), 48-59.

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Cell Proliferation and Cell Cycle Analysis

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In 2D cultures, cells were removed by incubation for 5 min at 37°C in 0.25% trypsin + 1 mM EDTA solution (Gibco, Invitrogen). In 3D cultures, aggregates were collected by centrifugation (5 min, 200g) and dissociated using trypsin-EDTA solution. Trypsin reaction was stopped by adding half a volume of FBS, and cells were counted in a
Malassez hemocytometer using trypan blue exclusion assay. The proliferative index was estimated as the ratio between viable cell counts 48 hour post-seeding and number of seeded cells. Each proliferative index was normalized to this one obtained for control cells and data were expressed as percentages.
For cell cycle analysis, harvested cells were washed twice in PBS containing 5 mM EDTA, and fixed for 45 min at 4°C in 1 mL 75% ethanol in PBS with 5 mM EDTA.
Cell lines were washed twice and suspended in PBS, 5 mM EDTA containing 0.1% TritonX100 (Sigma Aldrich) mixed with 40 µg RNase A (Sigma Aldrich) and 25 µg propidium iodide (PI, Sigma Aldrich) and incubated for 15 min protected from light.
The stained samples were analysed in Gallios flow cytometer (Beckman Coulter, France). Histograms were analysed using Wincycle software (PHOENIX flow systems, USA).

Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J.P., Rossi C, & Vayssade M. (2015). Betanin-Enriched Red Beetroot (Beta vulgaris L.) Extract Induces Apoptosis and Autophagic Cell Death in MCF-7 Cells. Phytotherapy research : PTR, 29(12).

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