V6130

Manufactured by Merck Group

The V6130 is a laboratory equipment product manufactured by Merck Group. It is a precision instrument designed for use in scientific and research applications. The core function of the V6130 is to perform accurate measurements and data collection tasks. No further details or interpretation of the intended use are provided.

Automatically generated - may contain errors

Lab products found in correlation

8 br camp, by Merck Group (1 mentions) S6883, by Merck Group (1 mentions) Pacap, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

3 protocols using v6130

Detailed Protocol for Peptide Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were obtained from Sigma Aldrich (Poole, Dorset, UK) or Fisher Scientific (Loughborough, UK). 8-bromo-cAMP (8-Br-cAMP, B5386, Sigma) was prepared fresh immediately before use. Pituitary adenylate cyclase-activating polypeptide-38 (PACAP, A1439, Sigma), the dominant peptide in all tissues examined, was used in these experiments. Vasoactive intestinal peptide (VIP, V6130, Sigma), PACAP, corticotrophin-releasing hormone (CRH, C3042, Sigma) and calcitonin gene-related peptide (CGRP, C0168, Sigma) were dissolved in 175 mM acetic acid and substance P (S6883, Sigma) in 50 mM acetic acid with 1% (w/v) bovine serum albumin (A8806, Sigma). Tetrodotoxin (TTX, NA-120, Enzo Life Sciences, Exeter, UK), dissolved at 1 mg/ml in 1% (v/v) acetic acid, was stored at −20°C and thawed immediately before use. Peptides were stored in aliquots at −80°C and defrosted immediately before use.

Moran A.W., Al-Rammahi M.A., Batchelor D.J., Bravo D.M, & Shirazi-Beechey S.P. (2018). Glucagon-Like Peptide-2 and the Enteric Nervous System Are Components of Cell-Cell Communication Pathway Regulating Intestinal Na+/Glucose Co-transport. Frontiers in Nutrition, 5, 101.

+ Open protocol

+ Expand

Corneoscleral Explants Pretreatment with VIP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before their storage (4°C), human donor corneoscleral explants dissected from the right eyes were treated with VIP (V6130; Sigma, St Louis, MO; 10⁻⁸ M in medium A), whereas those of the paired left eyes were used as controls and treated with medium A alone in 35-mm Petri dishes containing 3.5 mL of medium A under 5% CO₂/95% air at 37°C for 30 minutes. The corneoscleral explants were stored (4°C) for 4 days before being shipped to the Lions Eye Institute for cornea precutting.

Koh S.W., Coll T., Gloria D, & Sprehe N. (2017). Corneal Endothelial Cell Integrity in Precut Human Donor Corneas Enhanced by Autocrine Vasoactive Intestinal Peptide. Cornea, 36(4), 476-483.

+ Open protocol

+ Expand

Corneoscleral Explant VIP Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Before their storage (4°C), human donor corneoscleral explants dissected from the right eyes were treated with VIP (V6130; Sigma, St. Louis, MO; 10⁻⁸ M in medium A), whereas those of the paired left eyes were used as controls and treated with medium A alone in 35-mm Petri dishes containing 3.5 mL medium A under 5% CO₂/95% air at 37°C for 30 min. The corneoscleral explants were stored (4°C) for four days before being shipped to the Lions’ Eye Institute for cornea precut.

Koh S.W., Coll T., Gloria D, & Sprehe N. (2017). Corneal Endothelial Cell Integrity in Precut Human Donor Corneas Enhanced by Autocrine Vasoactive Intestinal Peptide. Cornea, 36(4), 476-483.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!