Safeclear xylene substitutes

Chemicals used for the staining of tissue sections were as follows: hydrogen peroxide, hydrochloric acid (HCl), methanol, Permount, polyvinylpyrrolidone (PVP) (Cat.#BP431-100), SafeClear Xylene Substitutes, Triton X-100, and potassium ferrocyanide trihydrate (Cat.#S25489) were purchased from Fisher Scientific (Hanover Park, IL). Sodium azide, sodium borohydride, proteinase K, phosphate-buffered saline (PBS), Trizma base, and 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Cat.#D5905) were purchased from Sigma (St. Louis, MO). Chemicals used for test tube studies were as follows: Ferric iron solution (iron Standard for AAS, 1,000 mg/L Fe in nitric acid), Sodium azide, and DAB were purchased from Sigma-Aldrich (Oakville, Ontario, Canada), whereas K₄Fe(CN)₆ (EM Science), TRIS (EM Science), HCl (BDH), and H₂O₂ (BDH) were purchased from VWR (Mississauga, Ontario, Canada). Proper attention to safety is required when handling these chemicals.