Kh2po4

The antiviral agents FAVI, IMA and MOLNU are products of MedChemExpress (Monmouth Junction, NJ, USA); WF, DG, DIP, D₂O, HSA (A8763, essentially globulin free), AGP (G9885), transferrin (T3309, containing physiological amount of iron) and human blood serum (H4522, male AB plasma) were purchased from Sigma–Aldrich (Merck KGaA, Burlington, MA, USA). Inorganic compounds such as KCl, NaCl, KH₂PO₄ and Na₂HPO₄ × 2 H₂O, boric acid, sodium trimethylsilylpropanesulfonate (DSS), 2-(N-morpholino)ethanesulfonic acid (MES) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were products of Molar Chemicals (Halásztelek, Hungary) or Reanal (Budapest, Hungary) in puriss quality.

All solvents were of analytical grade and used without further purification (except the solutions used for the DNA cleavage assay, vide infra). [RhCp*(μ-Cl)Cl]₂, [Ru(η⁶-p-cymene)(μ-Cl)Cl]₂, 5-chloro-8-hydroxyquinoline, D-proline, D-homoproline, aqueous formaldehyde (30% (v/v)), pic, phen, KNO₃, HNO₃, KOH, n-octanol, CD₃OD, D₂O, doxorubicin, EB, HSA (A8763, essentially globulin free), ct-DNA, DG, 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) were Sigma-Aldrich (St. Louis, MO, USA) products and were used without further purification. FHA was purchased from GenScript (Piscataway, NJ, USA). NaH₂PO₄, Na₂HPO₄, KH₂PO₄, KCl, NaCl, diethyl-ether, n-hexane, ethanol, ethyl acetate and methanol were purchased from Molar Chemicals (Halásztelek, Hungary) or Reanal (Budapest, Hungary). [RuCym(HQCl-L-Pro)Cl]Cl and [RhCp*(HQCl-L-Pro)Cl]Cl were used in some cases due to comparative reasons, and their preparation and characterization were reported in our previous work [25 (link)].

All solvents were of analytical grade and used without further purification. [Rh(η⁶-C₅Me₅)(μ-Cl)Cl]₂, [Ru(η⁶-p-cymene)(μ-Cl)Cl]₂, 5-nitro-8-hydroxyquinoline, L-proline, aqueous formaldehyde (30% (v/v)), bpy, tetrabutylammonium nitrate (TBAN), KH-phthalate, n-octanol, D₂O, doxorubicin, 2-(N-morpholino)ethanesulfonic acid (MES), human serum, Dulbecco’s Modified Eagle Medium (DMEM) and RPMI media, HSA (A8763, essentially globulin free), DG, 1-methylimidazole, 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) were Sigma-Aldrich (Darmstadt, Germany) products and were used without further purification. Ac-Ala-His-Ala-NH₂ (AHA) and Ac-Phe-His-Ala-NH₂ (FHA) peptides were purchased from GenScript (Piscataway, NJ, USA). HQCl-L-Pro was prepared according to our former report [12 (link)]. NaH₂PO₄, Na₂HPO₄, KH₂PO₄, KCl, KNO₃, NaCl, KOH, HNO₃, ethanol, ethyl acetate, dichloromethane, n-hexane, diethyl-ether, and methanol were purchased from Molar Chemicals (Halásztelek, Hungary) or VWR (Budapest, Hungary).