RhoA activity was analyzed using Rhotekin Rho binding domain (Upstate Biotechnology; Thermo Fisher Scientific, Inc.) bound to glutathione agarose beads to pulldown the active GTP-bound RhoA form from ovarian cell lysates in lysis buffer (20 mM HEPES, pH 7.5, 0.5% NP-40, 100 mM NaCl, 0.2% deoxycholic acid, 10% glycerol, and 10 mM MgCl2) supplemented with protease and phosphatase inhibitors (37 (link)). GTP-bound RhoA and total RhoA were evaluated by western blot analysis as above, using RhoA antibody (67B9; 1:1,000; Cell Signaling Technology, Inc.).
Rhoa antibody 67b9
Manufactured by Cell Signaling Technology
The RhoA antibody (67B9) from Cell Signaling Technology is a laboratory tool used to detect the RhoA protein. RhoA is a small GTPase that plays a role in regulating the cytoskeleton and cellular processes. The antibody can be used to identify and quantify RhoA expression in various experimental systems.
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Lab products found in correlation
2 protocols using rhoa antibody 67b9
1
RhoA Activity Assay in Ovarian Cells
2
Measuring Active RhoA by Pulldown Assay
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Active RhoA was measured with Rho-activation pulldown assay (Millipore) following manufacturer's instructions. Active RhoA was detected by immunoblotting using RhoA antibody (67B9, Cell Signaling), as described (7 (link)).
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