Magellan 400 xhr scanning electron microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Magellan 400 XHR Scanning Electron Microscope is a high-resolution imaging system designed for advanced materials analysis. It provides detailed surface imaging and characterization capabilities.

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Lab products found in correlation

Scd 050 sputter coater, by Oerlikon Balzers (1 mentions) Ace600 sputter coater, by Leica (1 mentions)

5 protocols using magellan 400 xhr scanning electron microscope

Characterization of Sheared PEDOT:PSS Particles

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Optical microscopy was used to analyze the size distribution of sheared
PEDOT:PSS particles dispersed in water. The open-source software, Fiji, was used
to calculate surfaces areas of all PEDOT:PSS particles in 7 samples of the same
PEDOT:PSS particle dispersion, totaling 451 particles. Only particles larger
than 1 μm were measured, as smaller sizes were difficult to resolve with
optical microscopy and can also be assumed to safely pass through any standard
gauge needle. The equivalent circular diameter (ECD) was calculated for each
particle by assuming that each irregularly shaped particle can be represented as
a circle of the same surface area. Scanning electron microscopy was performed on
lyophilized samples using a FEI Magellan 400 XHR Scanning Electron Microscope.
X-ray photoelectron spectroscopy was performed on dried samples using a PHI
Versaprobe III Scanning XPS Microprobe.

Feig V.R., Santhanam S., McConnell K.W., Liu K., Azadian M., Brunel L.G., Huang Z., Tran H., George P.M, & Bao Z. (2021). Conducting polymer-based granular hydrogels for injectable 3D cell scaffolds. Advanced materials technologies, 6(6), 2100162.

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Scanning Electron Microscopy of Mycobacterial Biofilms

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Biofilms were grown as described above, with the addition of circular glass coverslips to the bottom of the multiwell plate wells. Following biofilm formation, the biofilm media supernatants were removed and the biofilms were rinsed with water. Coverslips with adherent biofilms were removed from the multiwell plates, and the samples (planktonic and biofilm) were dried overnight. Planktonic samples were prepared by resuspending M. avium grown in liquid culture in PBS, then allowing the bacterial suspension to dry on circular coverslips overnight. Then, the samples were fixed with 2% glutaraldehyde in 0.1 M sodium cacodylate (pH 7.5) for 1 h. Samples were rinsed with 0.1 M sodium cacodylate (pH 7.5), fixed in 1% osmium tetroxide, then rinsed again three times with buffer. The samples were then dehydrated using a graded ethanol series, before drying with a critical-point dryer. The dried samples were mounted on SEM stubs and sputter coated with iridium to a thickness of 5 µm. Samples were imaged at the Notre Dame Integrated Imaging Facility using a Magellan 400 XHR scanning electron microscope (FEI, Hillsboro, OR, USA).

McManus W.R, & Schorey J.S. (2023). Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms. Pathogens, 12(12), 1427.

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SEM Analysis of Dehydrated Cell-Laden Hydrogels

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Electron microscopies were obtained from dehydrated scaffolds in an FEI Magellan 400 XHR Scanning Electron Microscope under high vacuum, with an accelerating 163voltage of 3.00 kV. The SEM sample was prepared by transferring a cell-laden peptide hydrogel onto a glass coverslip. The gel was then dehydrated by immersing it in a series of ethanol solutions of increasing concentrations. The dehydrated gels were then dried in the Automated Critical Point Dryer. The dried samples were sputter coated with 5 nm iridium (Ir) before imaging.

Pérez-Pedroza R., Al-Jalih F., Xu J., Moretti M., Briola G.R, & Hauser C.A. (2022). Fabrication of lumen-forming colorectal cancer organoids using a newly designed laminin-derived bioink. International Journal of Bioprinting, 9(1), 633.

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Evaluating Biocompatibility of Cell Transformations

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To investigate the biocompatibility of the uptake and washing methods, colony forming units (CFUs) were counted after 48 hours of incubation at 30 °C on YPD plates (in triplicates) after a dilution series to prevent overcrowding. The respective controls for chemical and electrical transformation were produced with the exact same amount of pipetting and washing steps as for the transformation experiments. In order to check for cellular damage as a result of the used treatment, cells were imaged using SEM (FEI Magellan 400 XHR Scanning Electron Microscope, USA). In preparation, cells fixed in 1% glutaraldehyde and 4% paraformaldehyde in 0.1 M cacodylic acid were dried on a cover slip and coated with a 4 nm gold layer for 30 seconds at 20 °C, 40 mA in a Balzers SCD050 sputter coater.

Hemelaar S.R., van der Laan K.J., Hinterding S.R., Koot M.V., Ellermann E., Perona-Martinez F.P., Roig D., Hommelet S., Novarina D., Takahashi H., Chang M, & Schirhagl R. (2017). Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells. Scientific Reports, 7, 5862.

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Hydrogel Nanostructure Characterization

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For characterization of the nanoscale and microscale structures in the hydrogels, samples were immersed in deionized water for 24 h prior to freeze-drying using a VirTis BenchTop Pro freeze-dryer (SP Industries, Warminster, PA). The films were affixed to SEM stubs using conductive carbon tape and then sputter coated with 4 nm of iridium using an ACE 600 sputter coater (Leica Microsystems, Buffalo Grove, IL). Samples were imaged using a FEI Magellan 400 XHR Scanning Electron Microscope (FEI Company, Hillsboro, OR). Secondary electron images were acquired using an Immersion lens and Through the Lens (TLD) detector typically with a voltage of 3 kV and a dwell time of 10.0 Î¼s. Data collection and analysis was carried out using an xT microscope control version 5.0.2.2666 build 2666.

Heedy S., Pineda J.J., Meli V.S., Wang S.W, & Yee A.F. (2022). Nanopillar Templating Augments the Stiffness and Strength in Biopolymer Films. ACS nano, 16(2).

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