Immunoblot analysis was carried out with the following primary antibodies: anti-Gli1 (Cell Signaling), anti-Gli2 (R&D systems), anti-Gli3 (9 (link)), anti-HA (Santa Cruz), anti-Myc (Santa Cruz), anti-Flag (Sigma), anti-Tubulin (Sigma), anti-Gapdh (Millipore), anti-ERK (Santa Cruz), anti-pAKT (Cell signaling), anti-pGSK3 (cell signaling), anti-p-β-Catenin (cell signaling). CA-AKT plasmid (myrAkt, Addgene), Bafilomycin A1 (Sigma) and MG132 (Calbiochem) were purchased. For immunoprecipitation, cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA and 1% NP-40, supplemented with complete mini protease inhibitor cocktail (Roche). After centrifugation the supernatants were incubated with the indicated antibodies overnight at 4°C, and the immunoprecipitates extensively washed with lysis buffer, eluted with SDS sample buffer and boiled for 5 minutes prior to analyses by immunoblotting.
Anti gli3
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-Gli3 is a laboratory reagent used for the detection and analysis of the Gli3 protein, which is a transcription factor involved in various cellular processes. It is a specific antibody that can be utilized in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to identify and study the Gli3 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti gli1, by Cell Signaling Technology (2 mentions)
Anti flag, by Merck Group (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti p β catenin, by Cell Signaling Technology (1 mentions)
Anti pgsk3, by Cell Signaling Technology (1 mentions)
Anti erk, by Santa Cruz Biotechnology (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
Complete mini protease inhibitor cocktail, by Roche (1 mentions)
Anti gli2, by R&D Systems (1 mentions)
Anti myc, by Santa Cruz Biotechnology (1 mentions)
Anti ha, by Santa Cruz Biotechnology (1 mentions)
1rdye 680cw goat anti mouse igg secondary antibodies, by LI COR (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
β catenin, by BD (1 mentions)
Plrp6, by Cell Signaling Technology (1 mentions)
Anti survivin, by Cell Signaling Technology (1 mentions)
Anti xiap, by Cell Signaling Technology (1 mentions)
Gant61, by Selleck Chemicals (1 mentions)
3 protocols using anti gli3
1
Immunoblot Analysis of Hedgehog and AKT Signaling
2
Midbrain Tissue Dissection and Protein Analysis
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The midbrain tissues were dissected from E13.5 mouse fetuses as described (Weinert et al., 2015 (link)). The dissected midbrain tissues were lysed with Radioimmunoprecipitation assay (RIPA) lysis buffer. The denatured protein samples were immunoblotted using anti-Gli3 (Santa Cruz Biotechnology), Gli1, p-LRP6, Dvl2, β-actin (Cell signaling), β-catenin (BD bioscience) and then with 1RDye® 800CW goat anti-rabbit IgG and 1RDye® 680CW goat anti-mouse IgG secondary antibodies (LI-COR). The images were captured by Odyssey® (LI-COR).
3
Genipin and GANT61 Modulate Apoptosis
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Genipin was purchased from Calbiochem (San Diego, CA, USA). GANT61 was purchased from Selleckchem (Houston, TX, USA). Protein G PLUS-Agarose and anti-BAX, anti-BCL2, anti-MCL-1, anti-UB, anti-BCL-Xl, anti-GLI3, anti-PCAF, anti-SHH, anti-PTCH, and anti-p53 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-XIAP, anti-NOXA, anti-PUMA, anti-BIM, anti-SURVIVIN, anti-BID, anti-cleaved PARP, anti-CASP3, anti-CASP9, anti-β-TrCP, and anti-GLI1 antibodies were purchased from Cell Signaling (Beverly, MA, USA). The anti-actin antibody was obtained from Sigma (St. Louis, MO, USA), the anti-SMO antibody was obtained from Abcam (Cambridge, UK), and the anti-ITCH antibody was purchased from BD science (San Jose, CA, USA). The secondary antibodies, anti-mouse IgG HRP and anti-rabbit IgG HRP, were obtained from Cell Signaling.
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