Trifluoroacetic acid tfa and solvents

tert-Butyl-3-hydroxypropanoate, methyl 3-mercaptopropionate, and ethyl methacrylate were purchased from Acros Organics. 2,2′-Azobis-(isobutyronitrile) (AIBN) and ethyl methacrylate were purchased from Sigma-Aldrich Co. Trifluoroacetic acid (TFA) and solvents were purchased from Thermo Fisher Scientific, Inc. The chemicals were used without further purification, with the exception of methacryloyl chloride which was distilled before use and AIBN which was recrystallized from hot methanol. Boc-amino ethyl methacrylate was synthesized according to the previous reported procedure.^{61 (link)1}H NMR was performed using a Varian MR400 (400 MHz), ¹³C NMR was performed using a Bruker NEO600 (600 MHz) and the data was analyzed using VNMRJ 3.2 and MestReNova. Gel permeation chromatography (GPC) analysis was performed using a Waters 1515 HPLC instrument equipped with Waters Styragel (7.8 × 300 mm) HR 0.5, HR 1, and HR 4 columns in sequence and detected by a differential refractometer (RI). Mueller Hinton broth (MHB, BD and Company) and phosphate buffered saline (PBS, pH = 7.4, Gibco) were prepared according to manufacturer instructions and sterilized prior to use. Human red blood cells (RBCs) (leukocytes reduced adenine saline added) were obtained from the American Red Cross Blood Services Southeastern Michigan Region and used prior to the out date indicated on each unit.