Tumor xenografts were fixed in formalin for immunohistochemical staining. Immunostaining for Ki67 was conducted using a rabbit anti-human Ki67 antibody (Cell Signaling, #9027, 1:400) on tumor sections. Staining was performed using the Vectastain ABC Kit (Vector Laboratories), according to the manufacturer’s instructions.
Rabbit anti human ki67 antibody
Manufactured by Cell Signaling Technology
Rabbit anti-human Ki67 antibody is a primary antibody that specifically recognizes the Ki67 protein, a well-established marker of cellular proliferation. This antibody can be used to detect and quantify Ki67 expression in various cell and tissue samples.
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Lab products found in correlation
2 protocols using rabbit anti human ki67 antibody
1
Immunohistochemical Analysis of Ki67 in Tumor Xenografts
2
Immunochemical Analysis of HCC Tissue Samples
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Carcinoma and adjacent liver tissues (n = 90) derived from HCC patients were from the First Affiliated Hospital, Zhejiang University School of Medicine (Hangzhou, China). The Institutional Review Board at Zhejiang University School of Medicine approved the protocol of this study, and all patients provided informed consent. Samples were fixed in 4% formalin and embedded in paraffin. Sections with a thickness of 4 μm were placed on glass slides for immunochemical or immunofluorescence staining analysis. Immunochemical or immunofluorescence staining analysis was performed as previously reported28 (link). Endogenous GP73 was tagged using mouse anti-human GP73 antibody (Abvona, Taipei, Taiwan), c-Myc was tagged using rabbit anti-human c-Myc antibody, Ki-67 was tagged using rabbit anti-human Ki-67 antibody and MMP-7 was tagged using rabbit anti-human MMP-7 antibody (Cell Signaling Technology). Images were captured using an Olympus FV1000 confocal microscope (Olympus Corporation, Japan).
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