Cell stimulating antibodies to CD3 (clone OKT-3), CD28 (clone AF-342-PB) and CD46 (clone 344519) were all purchased from R&D Systems. Neutralizing antibodies to IL-4 (clone 8D4-8) and IL-10 (clone JES3-19F1) were both from BD Pharmingen. Antibodies used in flow cytometric analysis were FITC-labeled anti-CD46 (clone MEM-258), BV421-labeled anti-CD122 (clone TU27), PE-labeled anti-CD132 (clone AG184) from BioLegend, and FITC-labeled anti-CD19 (clone 4G7-2E3) and APC-labeled anti-CD138 (clone 359103), both from R&D Systems, while the APC-labelled anti-CD25 (clone M-A251) was purchased from BD Pharmingen. Recombinant human IL-2 and IL-10 were purchased from Pepro-Tech and soluble CD46 was prepared as previously described.10 The MMP-9 inhibitor was purchased from Calbiochem (CAS 1177749-58-4) and used at the concentrations indicated in the respective experiments.
Apc labelled anti cd25 clone m a251
Manufactured by BD
The APC-labelled anti-CD25 (clone M-A251) is a monoclonal antibody that binds to the CD25 antigen. CD25 is the alpha chain of the interleukin-2 receptor and is expressed on activated T cells, B cells, and natural killer cells. This antibody is conjugated with Allophycocyanin (APC), a fluorescent dye, for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd46 clone 344519, by R&D Systems (2 mentions)
Il 4 clone 8d4 8, by BD (2 mentions)
Il 10 clone jes3 19f1, by BD (2 mentions)
Fitc labeled anti cd19 clone 4g7 2e3, by R&D Systems (2 mentions)
Apc labeled anti cd138 clone 359103, by R&D Systems (2 mentions)
Recombinant human il 2 and il 10, by Thermo Fisher Scientific (2 mentions)
2 protocols using apc labelled anti cd25 clone m a251
1
Stimulating T and B Cell Responses
2
Immune Cell Activation and Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell stimulating antibodies to CD3 (clone OKT‐3), CD28 (clone AF‐342‐PB) and CD46 (clone 344519) were all purchased from R&D Systems. Neutralizing antibodies to IL‐4 (clone 8D4‐8) and IL‐10 (clone JES3‐19F1) were both from BD Pharmingen. Antibodies used in flow cytometric analysis were FITC‐labeled anti‐CD46 (clone MEM‐258), BV421‐labeled anti‐CD122 (clone TU27), PE‐labeled anti‐CD132 (clone AG184) from BioLegend, and FITC‐labeled anti‐CD19 (clone 4G7‐2E3) and APC‐labeled anti‐CD138 (clone 359103), both from R&D Systems, while the APC‐labelled anti‐CD25 (clone M‐A251) was purchased from BD Pharmingen. Recombinant human IL‐2 and IL‐10 were purchased from PeproTech and soluble CD46 was prepared as previously described.10 The MMP‐9 inhibitor was purchased from Calbiochem (CAS 1177749‐58‐4) and used at the concentrations indicated in the respective experiments.
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