Anti tgn

Cell growth assay was performed, as previously described (Bin et al., 2016) . Briefly, after the siZIP7 treatment, cells were seeded in 6-well plates at a density of 2 Â 10 4 per well. On the next day, cells were treated with 10 pM of siZIP7. On each day of assay, cell fixation was performed with 4% paraformaldehyde, and fixed cells were stained with 500 ml of 0.1% crystal violet. The stained cells were lysed with 1 ml of 10% acetic acid, and absorbance at 450 nm was measured.
Fluorescence microscopy, in situ hybridization, and X-ray analysis For fluorescence microscopy, cells were cultured on Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA) and stained with the following antibodies: anti-V5 (Life Technologies), anti-FLAG (Sigma), anti-BIP (Abcam, Cambridge, MA), and anti-TGN (Abcam), as previously described (Bin et al., 2014a) . ER-Tracker Red and a Golgi tracker CellLight Golgi-RFP were used as the manufacturer's describes (Life Technologies). Images were taken with an LSM 700 confocal microscope (Carl Zeiss, Jena, Germany). In situ hybridization was carried out using Genostaff methods (Fukada et al., 2008; Ohashi et al., 2016) . X-ray analysis was performed as previously described (Fukada et al., 2008) .