The microchip fabrication was performed according to standard soft lithography and microfabrication methods using SU8-3035 negative photoresist (Microchem) and polydimethylsiloxane (RTV 615, Momentive). Briefly, a 10:1 mixture of PDMS prepolymer A and cross-linker B was poured on an SU8 mold and cured in an oven at 80 °C for 1 h after vacuum degassing. The PDMS microchip was punched and bonded with a premium-grade microarray glass slide (poly-L-lysine–coated; Thermo Fisher). The whole chip was further heated at 80 °C for another 2 h to complete thermal bonding.
Su8 3035 negative photoresist
Manufactured by MicroChem
Sourced in United States
SU8-3035 is a negative photoresist manufactured by MicroChem. It is designed for use in applications requiring thick, high-aspect-ratio and chemically-resistant features. The product is a solution of an epoxy-based polymer in a solvent.
Automatically generated - may contain errors
Lab products found in correlation
Sylgard 184 pdms base and curing agent, by Dow (2 mentions)
Poly l lysine coated, by Thermo Fisher Scientific (1 mentions)
Silgard 184, by Dow (1 mentions)
Rat tail collagen type 1, by BD (1 mentions)
Calcein pi cell viability assay kit, by Beyotime (1 mentions)
Polycarbonate porous membrane, by Cytiva (1 mentions)
Trypsin and edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8, by Apexbio (1 mentions)
Glipizide, by Merck Group (1 mentions)
Ultrasensitive rat insulin elisa kit, by Mercodia (1 mentions)
Sodium fluorescein, by Merck Group (1 mentions)
6 protocols using su8 3035 negative photoresist
1
Soft Lithography for Microchip Fabrication
2
Microfluidic Invasion and Metastasis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Previously developed invasion and metastasis microfluidic devices [41 (link), 42 (link)] were employed. A monolayer-invasion microfluidic chip and a multilayer-metastasis microfluidic chip were constructed of poly-dimethylsiloxane (PDMS, Silgard 184, Dow Chemical), which was replicated from SU-8 3035 negative photoresist (Microchem Corp.)-patterned wafers. Cell-inlet holes and culture chambers were created using differently sized punchers (1.5 mm and 3 mm diameter). After the PDMS chips were prepared, they were bonded irreversibly to a glass substrate after oxygen-plasma treatment for 90 s. Before use, the device was sterilized with UV light for 30 min.
3
Ginseng-based Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polycarbonate porous membrane (Whatman, UK), Polydimethylsiloxane (PDMS, Dow Corning, USA), SU-8 3035 negative photoresist (MicroChem, USA), Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA), fetal bovine serum (FBS, Gibco, USA), trypsin and EDTA (Gibco, USA), rat tail type-I collagen (BD, USA), Calcein/PI cell viability assay kit (Beyotime, China), cell counting kit-8 (CCK-8, APExBIO, USA), Annexin V-FITC apoptosis detection kit (Beyotime, China), Capecitabine (CAP, purity≥98%, Sigma, USA), Re, Rg3(S), Rh2(S) and CK ginsenosides (purity≥98%) were purchased from Sichuan China. All chemical reagents were mass spectrometry reagent grade.
4
Fabrication of Microfluidic Device
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microfluidic device is composed of a glass substrate and a polydimethylsiloxane (PDMS) membrane. The PDMS membrane was fabricated by repeated molding of the master, which was prepared by spin coating a 100-μm-thick layer of SU8-3035 negative photoresist (Microchem Corp., Newton, CA, USA) onto a glass wafer and patterned by photolithography. The Sylgard 184 PDMS base and curing agent (Dow Corning, Midland, MI, USA) were mixed thoroughly (10:1 by mass), poured onto the mold and cured in an oven at 80℃ for 1 hour. After cooling, the PDMS membrane was peeled from the master and trimmed to size. Inlet and outlet holes were punched out of the PDMS. Then the PDMS membrane was bonded to a glass slide after plasma treatments.
5
PDMS Microfluidic Device Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microchannels and chambers in the PDMS layers of microfluidic device were fabricated by replicate molding on the master. The master was prepared by spin coating SU8 3035 negative photoresist (Microchem, Newton, MA, USA) onto a plasma-treated glass wafer and patterned by photolithography. Sylgard 184 PDMS base and curing agent (Dow corning, Midland, MI, USA) were mixed thoroughly (10 : 1 by mass), degassed under vacuum, and poured onto the silane-treated master. The assembly was cured in an oven (80 °C, 1 h). After cooling, the PDMS was gently peeled from the master and trimmed to size. Holes were punched out of the PDMS to form reservoirs for introduction of liquid.
6
Microfluidic Assay for Insulin Secretion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
INS-1 cells, RPMI 1640 medium and 15% fetal bovine serum were purchased from National Infrastructure of Cell Line Resource, Chinese Academy of Medical Sciences. Penicillin and streptomycin were from Hyclone, USA. Basement membrane extractant (BME) was from R&D Systems, MN, USA. Sodium fluorescein and glipizide were from Sigma, St Louis, MO, USA. Calcein-AM and PI dyes were from Keygen biotech, Nanjing, China. Ultrasensitive Rat Insulin ELISA kit was from Mercodia, Sweden. PDMS prepolymer (Sylgard Silicone elastomer 184) was from Dow Corning, Midland, MI, USA. SU8 3035 negative photoresist was from Microchem, Newton, MA, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!