Konelab 20i thermo scientific autoanalyzer

Fasting glucose concentration was determined in blood plasma, while lipid parameters—total cholesterol, high-density lipoproteins cholesterol, low-density lipoproteins cholesterol, and triglycerides—were determined in the blood serum. All biochemical parameters were measured on the day of sampling with the Konelab 20i Thermo Scientific autoanalyzer (Vantaa, Finland), and intra- and inter-assay coefficients of variation were as follows: for FG, 1.13% and 1.99%; for TC, 1.72% and 2.27%; for HDL-C, 1.33% and 2.42%; for LDL-C, 2.7% and 4.9%; for TG, 1.74% and 4.08%, respectively. Serum aliquots were also stored at −80 ºC until sdLDL subclass determination by the precipitation method as described in detail by Hirano et al. [41 (link)]. Briefly, the serum sample was mixed 1:1 with a reagent containing heparin and MgCl₂, incubated at 37 °C, cooled, and centrifuged, and the concentration of LDL cholesterol in the obtained supernatant was measured using Konelab 20i Thermo Scientific autoanalyzer. The concentration of LDL in supernatant corresponded to the concentration of sdLDL fraction.