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Cd20 pe cy5 2h

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The CD20 PE Cy5 (2H) is a fluorochrome-conjugated monoclonal antibody that binds to the CD20 cell surface antigen. CD20 is a pan-B cell marker expressed on B lymphocytes throughout B cell differentiation.

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Lab products found in correlation

Cd14 brilliant violet 786, by BioLegend (2 mentions) Hla dr brilliant violet 711, by BioLegend (2 mentions) Cd11b apc h7, by BioLegend (2 mentions) Cd4 brilliant violet 605, by BioLegend (2 mentions) Cd8 brilliant violet 570, by BioLegend (2 mentions) Cd45 percp, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (2 mentions) Facs lysing solution, by BD (2 mentions)

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CD20-PE (6 citations)

2 protocols using cd20 pe cy5 2h

1

Multiparametric Flow Cytometry of Immune Cells

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Whole blood, whole bone marrow, and single cells from lymph nodes, colon biopsies, and rectum biopsies were first stained immediately with LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher). Cells were then stained using the following surface antigen antibodies with clone denoted in (), from BD biosciences unless otherwise stated: CD45 PerCP (D058-1283), CD3 PE CF594 (SP34-2), CD20 PE Cy5 (2H7), CD4 Brilliant Violet 605 (Biolegend, OKT4), CD8 Brilliant Violet 570 (Biolegend, RPA-T8), CD14 Brilliant Violet 786 (Biolegend, M5E2), HLA-DR Brilliant Violet 711 (G46-6), and CD11b APC-H7 (ICRF44). Red blood cells were lysed in whole blood samples using FACs lysing solution (BD). T cells were defined as live/CD45+/CD20−/CD14−/CD3+ and CD4+ and CD8+ T cells were further delineated to assess activation by HLA-DR expression. Neutrophils were defined as live/CD45+/CD3−/CD20−/CD14+/CD11b+/HLA-DR−/HighSSC cells (Figure 7).
Hensley-McBain T., Berard A.R., Manuzak J.A., Miller C.J., Zevin A.S., Polacino P., Gile J., Agricola B., Cameron M., Hu S.L., Estes J.D., Reeves R.K., Smedley J., Keele B.F., Burgener A.D, & Klatt N.R. (2018). Intestinal damage precedes mucosal immune dysfunction in SIV infection. Mucosal immunology, 11(5), 1429-1440.
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2

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole blood, whole bone marrow, and single cells from lymph nodes, colon biopsies, and rectum biopsies were first stained immediately with LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher). Cells were then stained using the following surface antigen antibodies with clone denoted in (), from BD biosciences unless otherwise stated: CD45 PerCP (D058-1283), CD3 PE CF594 (SP34-2), CD20 PE Cy5 (2H7), CD4 Brilliant Violet 605 (Biolegend, OKT4), CD8 Brilliant Violet 570 (Biolegend, RPA-T8), CD14 Brilliant Violet 786 (Biolegend, M5E2), HLA-DR Brilliant Violet 711 (G46-6), and CD11b APC-H7 (ICRF44). Red blood cells were lysed in whole blood samples using FACs lysing solution (BD). T cells were defined as live/CD45+/CD20−/CD14−/CD3+ and CD4+ and CD8+ T cells were further delineated to assess activation by HLA-DR expression. Neutrophils were defined as live/CD45+/CD3−/CD20−/CD14+/CD11b+/HLA-DR−/HighSSC cells (Figure 7).
Hensley-McBain T., Berard A.R., Manuzak J.A., Miller C.J., Zevin A.S., Polacino P., Gile J., Agricola B., Cameron M., Hu S.L., Estes J.D., Reeves R.K., Smedley J., Keele B.F., Burgener A.D, & Klatt N.R. (2018). Intestinal damage precedes mucosal immune dysfunction in SIV infection. Mucosal immunology, 11(5), 1429-1440.
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