1260 hplc system

Manufactured by YMC

The 1260 HPLC system is a high-performance liquid chromatography instrument designed for analytical and preparative applications. It features a modular design, allowing for the configuration of various components to meet specific analytical requirements. The system is capable of performing a range of chromatographic separations and analyses.

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Lab products found in correlation

Maxis q tof mass spectrometer, by Bruker (1 mentions) C18 column, by YMC (1 mentions) Avance 700 spectrometer, by Bruker (1 mentions) U 2910 spectrometer, by Hitachi (1 mentions) Ods c18 column, by YMC (1 mentions)

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1260 system (2 citations) HPLC system (2 citations)

3 protocols using 1260 hplc system

Detailed Analytical Techniques for Compound Characterization

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U-2910 spectrometer (Hitachi) was used to record the UV spectra of compounds 17–21. The 1D and 2D NMR spectra of compounds 17–21 were obtained with a Bruker Avance-700 spectrometer. All of the mass spectra were acquired on a Bruker MaXis Q-TOF mass spectrometer. Semi-preparative HPLC was performed on an Agilent 1260 HPLC system with a C₁₈ column (YMC, 10 mm × 250 mm, 5 μm).
Strains and constructed plasmids utilized are shown in Supporting Information Tables S3‒S4. LB medium was added with an additional antibiotic at a concentration of 50 μg/mL carbenicillin or 50 μg/mL kanamycin when necessary. Solid CD Medium was used for incubations of A. nidulans A1145 and Aspergillus sp. SCSIO SX7S7. The mutant strains of SCSIO SX7S7 were grown at Solid CD Medium with additional 200 μg/mL hygromycin (Hyg). Analytical or chromatographic-grade chemicals and solvents were used in this study.

Yang J., Zhou Z., Chen Y., Song Y, & Ju J. (2023). Characterization of the depsidone gene cluster reveals etherification, decarboxylation and multiple halogenations as tailoring steps in depsidone assembly. Acta Pharmaceutica Sinica. B, 13(9), 3919-3929.

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Vitamin C Quantification in Tomatoes

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The content of vitamin C was determined according to a previously reported method [37 (link)]. Two hundred fifty milligrams of lyophilized tomato pulp with skin was extracted with 3% metaphosphoric acid by sonication three times for 10 min each. The extract was centrifuged at 5700× g at 4 °C for 10 min, and supernatant was filtered with a 0.45 μm filter. HPLC analysis was performed on Agilent 1260 HPLC system and YMC ODS C18 column (4.6 × 250 mm, 4 μm) with 0.2 M KH₂PO₄ (pH 2.2; solvent A) and methanol (solvent B). The gradient elution was applied as follows: 0 to 15 min, 0% B; 15 to 23 min, 100% B; 23 to 24 min, 0% B; 24 to 25 min, 0% B. The flow rate was 0.7 mL/min and injection volume was 20 µL. Column temperature was 30 °C, and absorbance were measured at 254 nm.

Kang D.M., Kwon J.M., Jeong W.J., Jung Y.J., Kang K.K, & Ahn M.J. (2022). Antioxidant Constituents and Activities of the Pulp with Skin of Korean Tomato Cultivars. Molecules, 27(24), 8741.

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Quantification of Rosmarinic Acid in Ethanolic Extract

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The ST ethanolic extract was investigated with regard to its content in rosmarinic acid, used as a marker compound. Sample and reference standard solutions were prepared using 2 mg in 2 mL of 30% methanol. The HPLC was recorded under the following conditions: model–Agilent Technologies 1260 HPLC system; column: YMC Hydrosphere C18 (150 × 4.6, 5 μm); Detector: UV–Vis; mobile phase: (A) 0.1% formic acid in water with a gradient elution of 90%–10%, and (B) 0.1% formic acid in acetonitrile with a gradient elution of 10%–90%; injection volume: 10 μL; method time: 25 min; and flow rate: 1.0 mL/min; detector: UV (at 330 nm). The calibration curve was extracted using standard rosmarinic acid at concentrations between 0.125 and 0.5 μg/mL.

Gu M.J., Lee H.W., Yoo G., Kim D., Choi I.W., Kim Y, & Ha S.K. (2023). Protective effect of Schizonepeta tenuifolia Briq. ethanolic extract against UVB-induced skin aging and photodamage in hairless mice. Frontiers in Pharmacology, 14, 1176073.

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