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Metal halide lamp

Manufactured by Philips
Sourced in Germany

Metal halide lamps are a type of electric lamp that produce light through the use of mercury and metal halides. They are known for their high-intensity, energy-efficient illumination and are commonly used in commercial and industrial applications.

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Lab products found in correlation

5 protocols using metal halide lamp

1

Comparing Abiotic Stress Responses in Tomato Cultivars

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Solanum lycopersicum var. Lyterno (Rijk Zwaan Welver GmbH, Germany) and S. pennellii LA0716 (Tomato Genetics Resource Center, UC Davis, CA, US) were used to compare the effects of abiotic stress.
In brief, plants were grown from seeds in environmental chambers (Hühren Kälte-Klima-Elektrotechnik, Erkelenz, Germany), equipped with metal halide lamps (Philips, Hamburg, Germany), following the protocol described in Junker-Frohn et al. (2019 (link)), under controlled conditions of 22/18C during day and night, a relative humidity of 50% and 200μmolm-2s-1 photons light intensity for 10 h per day.
Plants were sown in rock wool plugs ( 2×2×4  cm; Grodan, Roermond, The Netherlands), that were four times prewashed with deionized water. Seeds were placed around 0.5 cm below the surface of the rock wool plugs. A total number of 150 seeds per species were seeded. The plugs were watered with water for 16 days. 84 seedlings per species, which already developed the first true leaf, were then transferred to rock wool blocks ( 7.5×7.5×6.5  cm) (Grodan, Roermond, The Netherlands), that were three times prewashed with deionized water. Afterwards, the seedlings were fertilized with half-strength Hoagland solution for 14 days, followed by full-strength Hoagland solution [ 5mMKNO3 , 5 mM Ca( NO3)2 , 2 mM MgSO4 , 1mMKH2PO4 , 90μM FeEDTA, plus micronutrients] for further 11 days.
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2

Coral Cultivation and Acclimation Protocol

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Colonies of P. damicornis were collected by SCUBA diving at water depths of 3–5 m from two fringing reefs, Luhuitou (LHT; 18°12′7′′N, 109°28′5′′E) and Houhai (HH; 18°16′40′′N, 109°44′3′′E), located at the southern tip of Hainan Island in the South China Sea (Supplementary Fig. S1, Tables S1 and S2). Colony replicates were generally separated by 2–3 m across each reef. The collected corals were transferred to the indoor husbandry facility at the Third Institute of Oceanography, Ministry of Natural Resources, China, and cultivated in an aquarium with 1000 L of recirculated artificial seawater (ASW) at a temperature of 26°C and photosynthetically active radiation of 150 μmol photons/m2/s provided by metal halide lamps (Phillips, Amsterdam, Netherlands) over a 12-h/12-h light/dark cycle. To minimize perturbations from environmental sampling, the coral colonies were grown in the aquarium for 6 months, followed by fragmentation and further acclimation for two more months prior to the experimental manipulation. No changes in dominant symbiont genotype were found during the coral maintenance [10 (link)].
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3

Controlled Growth of Capsicum Cultivars

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Capsicum annuum L. var. annuum cv. Mazurka (Rijk Zwaan Welver GmbH, Germany) and Capsicum chinense Jacq. cv. CAP 1035 (Genetics Resource Center IPK Gatersleben, Germany) were grown from seeds in environmental chambers (Hühren Kälte-Klima-Elektrotechnik, Erkelenz, Germany), equipped with metal halide lamps (Philips, Hamburg, Germany), following the protocol described elsewhere [52 (link)], under controlled conditions of 24/18 ℃ during day and night, a relative humidity of 55% and 300 µmol m−2 s−1 photons light intensity for 10 h per day.
In brief, plants were sowed in rock wool plugs (2 × 2 × 4 cm; Grodan, Roermond, The Netherlands), that were four times prewashed with deionized water. Seeds were placed around 0.5 cm below the surface of the rock wool plugs. The plugs were watered with 1/4 Hoagland solution for 14 days, followed by half-strength Hoagland solution for 14 days. A total of 54 seedlings per species, which had already developed the first real leaf, were transferred to rock wool blocks (7.5 × 7.5 × 6.5 cm) (Grodan, Roermond, The Netherlands), that were 3 times prewashed with deionized water. Afterwards, the seedlings were fertilized with full-strength Hoagland solution (5 mM KNO3, 5 mM Ca(NO3)2, 2 mM MgSO4, 1 mM KH2PO4, 90 µM FeEDTA, plus micronutrients).
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4

Photocatalytic Oxidation of Hypophosphite

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The photocatalytic performance of g-C3N4/Ti4O7 was characterized by photocatalytic oxidation of hypophosphite with the concentration of hypophosphite measured by ion chromatography using a 732 IC detector. A metal-halide lamp (35 W, Philips) with the light strength of ~5 mW cm−2 was used as the light source and a UV-cutoff filter of 420 nm was used to provide the visible light with the wavelength over 420 nm. Before the photocatalytic experiments, g-C3N4/Ti4O7 with various mass ratios (10 mg) dispersed in 100 mg L−1 hypophosphite aqueous solution (100 mL) were continuously stirred for 30 min in the dark to achieve the adsorption–desorption equilibrium. After that, the visible light photocatalytic oxidation of hypophosphite was conducted with the above solutions exposing to the visible light irradiation and sampling conducted at 1 h intervals over 6 h experimental period. More detailed information about the photocatalytic experiment can be found in our most recent paper (Guan et al., 2018 (link)). The oxidation efficiency of hypophosphite (η) was calculated using the following equation (Guan et al., 2017 (link)):
η=C0-CtC0×100%
where C0 and Ct represent the concentrations of hypophosphite at initial and given time, respectively.
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5

Artificial High-Light Protocol for Plant Research

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The high-light treatment was provided by supplementing normal light with a metal-halide lamp (Philips) under a short-day (8 h of illumination) photoperiod, and plants were arranged randomly to prevent shading. The light level varied between 50 and 100 μmol m−2 s−1 in the low-light conditions, while the light intensity was constant at 700 μmol m−2s−1 in the artificial high-light conditions. Unless otherwise stated, samples were all collected at the end of the light period of each day. Light measurements were determined using a digital lux meter sensor.
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